LOCUS AK074962 3587 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90481 fis, clone NT2RP3002900, moderately
similar to Membrane protein p24B precursor.
ACCESSION AK074962
VERSION AK074962.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3587)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..3587
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3002900"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000081997"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 208..882
/codon_start=1
/protein_id="BAC11318.1"
/translation="MPRPGSAQRWAAVAGRWGCRLLALLLLVPGPGGASEITFELPDN
AKQCFYEDIAQGTKCTLEFQVITGGHYDVDCRLEDPDGKVLYKEMKKQYDSFTFTASK
NGTYKFCFSNEFSTFTHKTVYFDFQVGEDPPLFPSENRVSALTQMESACVSIHEALKS
VIDYQTHFRLREAQGRSRAEDLNTRVAYWSVGEALILLVVSIGQVFLLKSFFSDKRTT
TTRVGS"
BASE COUNT 1040 a 638 c 663 g 1246 t
ORIGIN
1 ctcttttacc tccccaggtc cgcccgtctg cgcccctcac aggaagccgg agggtcgctc
61 tgatcccgaa tctcccacag gcgtgaacct gctctgctgt gtatctttgc ggggtggcct
121 gcgctgaggc ctgccgcgcg cggtgagtcc gcgcagacct gaccctgcgt ctcgcagctc
181 ggttgaggcc gccgccgcct tctcgggatg ccgcggccgg ggtccgcgca gcgctgggcg
241 gccgtcgcgg gccgttgggg gtgcaggctg ctcgcactgc tgctactggt gcctggaccc
301 ggcggcgcct ctgagatcac cttcgagctt cctgacaacg ccaagcagtg cttctacgag
361 gacatcgctc agggcaccaa gtgcaccctg gagttccagg tgattactgg tggtcactat
421 gatgtagatt gtcgattaga agatcctgat ggtaaagtgt tatacaaaga gatgaagaaa
481 cagtatgata gttttacctt cacagcctcc aaaaatggga catacaaatt ttgcttcagc
541 aatgaatttt ctactttcac acataaaact gtatattttg attttcaagt tggagaagac
601 ccacctttgt ttcctagtga gaaccgagtc agtgctctta cccagatgga atctgcctgt
661 gtttcaattc acgaagctct gaagtctgtc atcgattatc agactcattt ccgtttaaga
721 gaagctcaag gccgaagccg agcagaggat ctaaatacaa gagtggccta ttggtcagta
781 ggagaagccc tcattcttct ggtggttagc atagggcagg tatttctttt gaaaagcttt
841 ttctcagata aaagaaccac cacaactcgt gttggatcat aactacgttt tgagaattga
901 tgcaccattg ccactgtaat attgctgtcc tctaattaat tttaggtact gaagaactta
961 atattggcaa catttttaaa tccttactca tacacttgtt gggagggatg tacaatgcat
1021 attcccaaac tgtggaaagg acaccttttt ttatttgtaa aggtggaaaa actttggaac
1081 tcattttggg ctattcatgt taaatattca acaccaatga tctactctgt tcgcagttgt
1141 ttatatctac tcttcgcaca ctaaactttg gtattttgat tccttttaac catttaagac
1201 tacttttctt ataggtagtt gatattttaa aaactttaga tttaatgtct acatgtgtta
1261 gggaggaaga aaattgcctt ttaattgtta ataagaaaac caaatgtgat gaactgtagc
1321 ccaagcccta ttctgcactg ttcagtttta tggaggaaaa ataaatctac cataggaatg
1381 ttagttaata ttgatatatc atggtaaaat tgatttctca ctagcttaga aaatgtcaga
1441 cttttgtttt tggggtttat aatttaaacc agctatgcta ttttttcata aaggcatttg
1501 tagtacacag aaaacagtag tttcagtagt gtaaaagagt ttatacaggc cttaaatatc
1561 agactttgta acaggtagaa atattacaga ataatttaag acactacaat gggggcaaat
1621 gaaataggaa aatttttagt gagttacacg tactcattac attttcagtg cttttacaag
1681 gaaaaaaggt gatatgttta attttaaaat tttaattggc tagctcttgc ccttatatga
1741 ctttaatgtc tatgagtcat tcccagctta aattaacaat tgttagtatt agtctcacac
1801 ataagtgcca tacattttat cctcatggat gtgatgcact gaaaagttag tcgctctcct
1861 tttttctttt ttttgtcgtg catattttat ttctgtagtt tctggttagc taccctaaag
1921 tgatttaaaa atttagaatg ctttgtgttt cctatttggt aatcttcatt gacttttctt
1981 tagttaatga gtattaaata gtgcatattc tgtagactat agggtttaca ttgtgttgca
2041 tcttataact tgtatagatt gagctgattg aaataagatt ttgttccaag tattatctga
2101 tagaatacaa gatgattcaa aattatatag atatttaaag cttttctgct gttttttttt
2161 tttttttaat tgcaactgct tttctgccgt gcctctcttc cctacccaaa agtgatgagt
2221 tctgaacaag acaagactgt catattgtag agactttggt atgtgatacc atagaatact
2281 gattggatag ccatcctagt cacttaccaa tactgactag aagttaactc ttaattctaa
2341 gctatcttaa aatgcatata tatacttctt gcatggaaga gcaaaacaaa ttcaagttgt
2401 catgcctgat aatttcagat gccaccgtat agcaaagggt gaacatgttt tcaacccttt
2461 aactttttac ggtgtttgaa gaccagctac tccttaatat ttatcaatgg attaagaagt
2521 ttaagatttt gcagatttat caatttgggt ttttgtactg aagttgtctt gcggctttgc
2581 aagtgtccct ttatatttaa atttgaaagt tgtaagcact gatgttaatg tgattggtca
2641 gcatgggcat atgtaaaatg tccttttctg gttgcctctc tatgctattg tgttcagata
2701 cttacaccat aattaaacag taagttatag acttgctgag tttggcatag atagtgcact
2761 catttaatct gtgcctctca aaacttcaga atattagcat attaccacaa ataatttttg
2821 gtgaaactat tgagatatta aaatttttga aatcactact gttacctgtt atagaaaata
2881 gtgttggctt agtctagtct ctgtgtaact ggttacattt tgatggttgt ctatactcaa
2941 ctggatatgt gtatgtaaat tagaaaatac atacctatcc agacataaat gctaagtaac
3001 atttttttct tcctccaact acataatttg tagctcatca tttttcctta atcctttcct
3061 aacttgttgc agcagtttga atttcccaga tatttatgtt tgaacataat ggctcagaat
3121 acatatttga acatcatagt tgtatatatt tttaaagtag aataaataat ggaaagcaac
3181 ttgatataca agtttatact aaaatttcaa ctgtgtgaag atacatttta aggcattact
3241 ttgctaataa tctacacatt cttttttcta ttaaataaac aaatggtttg tggttcacag
3301 ctttcatcca agcttggtga taatcaattt tgaaaataca gtggactgaa aagcaaattt
3361 attctttcga agatttcatt taaatctctg tactatgcat ttttgtttca gtttgtaaat
3421 gtgtaacatt tatagttaaa taggacaaag caggccctat gactattgtt ttcaaaaaat
3481 taatatataa ataattcaga ggggtttttc atttactttt gaaaagaaat ttctttaaca
3541 ggattgcacc tatcaatttc ttgtagaata aaaaaaaatc tttcaac
//