LOCUS AK074912 3487 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90431 fis, clone NT2RP3000676, weakly similar
to PUTATIVE MITOCHONDRIAL CARRIER YMR166C.
ACCESSION AK074912
VERSION AK074912.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3487)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..3487
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP3000676"
/clone_lib="NT2RP3"
/db_xref="H-InvDB:HIT000081947"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 175..1119
/codon_start=1
/protein_id="BAC11287.1"
/translation="MEDKRNIQIIEWEHLDKKKFYVFGVAMTMMIRVSVYPFTLIRTR
LQVQKGKSLYHGTFDAFIKILRADGITGLYRGFLVNTFTLISGQCYVTTYELTRKFVA
DYSQSNTVKSLVAGGSASLVAQSITVPIDVVSQHLMMQRKGEKMGRFQVRGNPEGQGV
VAFGQTKDIIRQILQADGLRGFYRGYVASLLTYIPNSAVWWPFYHFYAEQLSYLCPKE
CPHIVFQAVSGPLAAATASILTNPMDVIRTRVQVEGKNSIILTFRQLMAEEGPWGLMK
GLSARIISATPSTIVIVVGYESLKKLSLRPELVDSRHW"
BASE COUNT 792 a 941 c 857 g 897 t
ORIGIN
1 attttccggt tccggcagta ccggtagccc agtcgagatg gaggaagatg cgaccggcag
61 acggcattcg ctgggaacga cggatagact gggggctgcg gcctagaggt ccgggcttgg
121 agttcgcctc agacgcggtg gagccgccgg agtctgagaa ggtcttcagg caccatggag
181 gacaaacgca acatccagat catcgagtgg gaacacctgg acaagaagaa gttctacgtg
241 tttggtgtgg caatgacaat gatgatccgt gtcagtgtct acccattcac cctcatccgc
301 acccggttgc aagttcagaa ggggaagagc ctctaccatg ggaccttcga tgccttcatc
361 aagatcctgc gagcagatgg tatcactggc ctctaccgag ggttcctggt caataccttc
421 accctcatct ctggccagtg ttatgtcacc acttatgagc tcacccggaa gtttgtagct
481 gactacagcc agagtaacac agtcaaatca ctggtggctg gtggctcagc ctcccttgtg
541 gcccagagca tcacagtgcc cattgatgta gtctcccagc acctgatgat gcaacgcaag
601 ggtgagaaaa tgggccgctt tcaggtgcgg gggaacccag agggacaagg ggtagttgcc
661 tttggccaaa ccaaggacat catcaggcag atcctgcagg ctgatggact tcgcggcttc
721 tatcgaggct atgtggcttc actgcttacc tatatcccaa acagtgctgt ctggtggccc
781 ttctatcact tctatgcaga gcagctctcc tacctgtgtc ctaaggagtg ccctcacatt
841 gtctttcaag ctgtctcggg gcccctggct gcagccactg cctccatcct caccaatccc
901 atggatgtca tacgaacccg tgtgcaggtt gagggcaaga actccatcat cctgaccttc
961 agacagctga tggcagaaga agggccttgg ggcctcatga agggcctctc ggccagaatc
1021 atctcagcca caccttccac cattgtcatt gtggtgggct atgagagcct caagaaactc
1081 agcctccgac ctgagctggt ggactcgaga cactggtaac cagtggtggg gagagaagcc
1141 tgctgttttc cacactaccg tgggtcaggg gcagagtgga gaggacagca ccctctccag
1201 gtgctcccac cacacaccca gccctgccct gggccaagtg gcctatctgg gatagggata
1261 gagactttga actgctcttg ctgaagaggc tccacgcctg gatcccttgc ccccactatt
1321 taaaattctc ttctgagctg ggctccctca ctcagtccct gtatttgata ctggcctaaa
1381 gaccccaccc cccaccctgc cagcccttct tctggcttcc ccttccatct gtgtccctga
1441 gaccctgaga agagctgtac atagagcttg cttactacca ctggttcttc ctcttgggct
1501 ttcagcccag actccaagca gctgctatca accctctctc ccttcatctc ttagccttgc
1561 ttatttttat tttgggaccg agctgcccac tagatgactc tgcttttccc tgcatttggg
1621 gctaaggtgc caggtactta tttgcacagg gagcaggagc agcaaaaaat ctctggttct
1681 ccagagcact cgtcctctct ttggaggggt tattaggttg ggagaaatgt tgatactttt
1741 gttttgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgttttaaca
1801 tctgtgaacc aggctattag tcctgctaaa gcgccaatcc tgctgtcaga gctcaccccc
1861 ttcctaagac aggtagaaaa atgtaatgta gctttttcca caagccactt ccctgtccct
1921 tcagtctcag gagccctagg agagtctaag ctggggcatc ccctggccca gaggactccc
1981 gtggtgggca cagttctaag tggatcaggc tgtcttgggt gcactggact tggagcacta
2041 ccttgagaag tcaggttgag aaagtagttg atctagaagg caacaagtgg gcatgtgttc
2101 cccagcacat tacccaggcc agcagagcca aacctaggag agggcagtgg gtagattctc
2161 tgccccaggc agccatgaca tacacataaa taccccaatc actcagactt acggcaacaa
2221 gtgttgtctc actatggtga tctctaagat ccacatcact ggatgcgtag tcatcccagt
2281 catggtaccc tgtggaggaa tgctggaaga acataaagag cagttcagaa agtcacccaa
2341 taccaggacc actgcattta ccagcctgat actgccaaga ttatctgatg ctctcctcag
2401 gagctaggag aggagtgctc cttcctccct accgctactc tccccaagcc tgtgttgcag
2461 gtagagaggt gcagcaaata gagaaggcat gtcaaaccct gcatttctac ctgagacgtg
2521 tgacctggat gatcctccaa accctattgg tcccaccccc tgggaaaggc catggtgcca
2581 gtttgaaagg tgctagctac ctgaagcctt gatatttctt catggctgcc gcacattctt
2641 ccaccttggc cagaacaggt tctgaaaacc acttctctac cttcaccacc accactgccc
2701 atcttgatct ctttgagggt tttcccattt cacttgatct tatttttgtt tatcccttcc
2761 tgcactttgt caagagagtc ctccagtttc tatccaggaa tgttcacatc caaagggttg
2821 gacccacgga tcattctgaa tcttcctgcc cctcctcact gcttaaccct gagaaccaca
2881 aatataatgg aagcagttcc ccccaccctc accccatctc tttaagctca tcctagcaag
2941 acctctagag accctagaga ctcgacttta gtccttcccc gccatggcac agtggggaag
3001 gtgtcaatgg ggagtgtcac ggacaggagg taggatcctg ccgctcgcgt cttagtgttt
3061 ctccctcaag actttccttc tgttttgttg tcttgtgcag tattttacag cccctcttgt
3121 gtttttcttt atttctcgta cacacacgca gttttaaggg tgatgtgtgt ataattaaaa
3181 ggacccttgg cccatacttt cctaattctt tagggactgg gattgggttt gactgaaata
3241 tgttttggtg gggatgggac ggtggacttc cattctccct aaactggagt tttggtcggt
3301 aatcaaaact aaaagaaacc tctgggagac tggaaacctg attggagcac tgaggaacaa
3361 gggaatgaaa aggcagactc tctgaacgtt tgatgaaatg gactcttgtg aaaattaaca
3421 gtgaatattc actgttgcac tgtacgaagt ctctgaaatg taattaaaag tttttattga
3481 gcccccg
//