LOCUS AK074874 2571 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ90393 fis, clone NT2RP2005597, highly similar
to Protein O-mannosyl-transferase 1 (EC 2.4.1.109).
ACCESSION AK074874
VERSION AK074874.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2571)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..2571
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2005597"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081909"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 161..2338
/codon_start=1
/note="highly similar to Protein O-mannosyl-transferase 1
(EC 2.4.1.109)"
/protein_id="BAG52022.1"
/translation="MWGFLKRPVVVTADINLSLVALTGMGLLSRLWRLTYPRAVVFDE
VYYGQYISFYMKQIFFLDDSGPPFGHMVLALGGYLGGFDGNFLWNRIGAEYSSNVPVW
SLRLLPALAGALSVPMAYQIVLELHFSHCAAMGAALLMLIENALITQSRLMLLESVLI
FFNLLAVLSYLKFFNCQKHSPFSLSWWFWLTLTGVACSCAVGIKYMGVFTYVLVLGVA
AVHAWHLLGDQTLSNVCVFCHLLARAVALLVIPVVLYLLFFYVHLILVFRSGPHDQIM
SSAFQASLEGGLARITQGQPLEVAFGSQVTLRNVFGKPVPCWLHSHQDTYPMIYENGR
GSSHQQQVTCYPFKDVNNWWIVKDPRRHQLVVSSPPRPVRHGDMVQLVHGMTTRSLNT
HDVAAPLSPHSQEVSCYIDYNISMPAQNLWRLEIVNRGSDTDVWKTILSEVRFVHVNT
SAVLKLSGAHLPDWGYRQLEIVGEKLSRGYHGSTVWNVEEHRYGASQEQRERERELHS
PAQVDVSRNLSFMARFSELQWRMLALRSDDSEHKYSSSPLEWVTLDTNIAYWLHPRTS
AQIHLLGNIVIWVSGSLALAIYALLSLWYLLRRRRNVHDLPQDAWLRWVLAGALCAGG
WAVNYLPFFLMEKTLFLYHYLPALTFQILLLPVVLQHISDHLCRSQLQRSIFSALVVA
WYSSACHVSNTLRPLTYGDKSLSPHELKALRWKDSWDILIRKH"
BASE COUNT 506 a 724 c 746 g 595 t
ORIGIN
1 gaacgttgag cagggcggtg ggtggtgcgg agtgccgagc ggcctcaccc ccaaccgtcg
61 gcccagtcgg acggttccga ggcgttgccg ggagccgggc gcggctctgt gtggactcgg
121 agaaacgcgg ggcgtctgcc tgagcccgct tttctacaag atgtggggat ttttgaagcg
181 ccctgtagtg gtgacggctg acatcaactt gagccttgtg gccctgactg ggatggggtt
241 actgagccgg ctgtggcgac tcacctaccc gcgggctgtg gtttttgacg aagtatatta
301 tgggcagtac atctcttttt acatgaaaca aatcttcttc ttggatgaca gtgggccgcc
361 atttggccac atggtgctgg ccttgggagg ttatttagga ggattcgatg gcaatttttt
421 gtggaacaga attggagcag aatacagtag caacgtgcct gtgtggtccc tgcgcctgct
481 gccagcactc gcgggggcct tgtcggtccc catggcctac cagatagtgt tggagctcca
541 cttttctcat tgtgccgcca tgggagctgc tctgttgatg cttatcgaga atgctctcat
601 cactcagtca aggctaatgc ttttggaatc agtgttaata tttttcaatc tattggccgt
661 gttgtcctac ctgaagttct tcaactgcca aaagcacagc cctttttctc tgagctggtg
721 gttctggcta acactgacag gggtcgcttg ttcctgtgca gtgggcatca agtacatggg
781 tgtgttcacg tacgtgctcg tgctgggtgt tgcagctgtc catgcctggc acctgcttgg
841 agaccagact ttgtccaatg tctgtgtgtt ctgtcacttg ctcgcccgag cagtggcttt
901 gctggtcatc ccggtcgtcc tgtacttact gttcttctac gtccacttga ttctagtctt
961 ccgctctggg ccccacgacc aaatcatgtc cagtgccttc caggccagct tagagggagg
1021 actagctcgg atcacccagg gtcagccact ggaggtggcc tttgggtccc aggtcactct
1081 gaggaacgtc tttgggaaac ctgtgccctg ctggcttcat tcccaccagg acacctaccc
1141 catgatatat gagaacggcc gaggcagctc ccaccagcaa caggtgacct gttacccctt
1201 caaagacgtc aataactggt ggattgtaaa ggatcccagg aggcaccagc tggtggtgag
1261 cagccctccg agacctgtga ggcacgggga catggtgcag ctggtccacg gcatgaccac
1321 ccgctccctg aacacgcatg atgttgcagc ccccctgagc ccccattcac aggaggtctc
1381 ctgctacatt gactataaca tctccatgcc cgcccagaac ctctggagac tggaaattgt
1441 gaacagagga tctgacacag acgtctggaa gaccatcctc tcagaggtcc gctttgtgca
1501 cgtgaacact tccgctgtct taaagctgag cggggctcac ctccctgact gggggtatcg
1561 gcaactggag atcgtcgggg agaagctgtc ccggggctac cacgggagca cggtgtggaa
1621 cgtggaggag caccgatacg gcgcgagcca ggagcagagg gagcgggaac gggagctgca
1681 ctcacctgcg caggtggacg tcagcaggaa cctcagcttc atggcgagat tctcggagct
1741 gcagtggagg atgctggcgc tgagaagtga tgactcggaa cacaagtaca gctccagccc
1801 actggagtgg gtcaccctgg acaccaatat tgcctactgg ctgcacccca ggaccagcgc
1861 tcagatccac ctacttggaa acatagtgat ctgggtttcg ggcagcctcg ctctggccat
1921 ctacgccctg ctgtccttgt ggtacctgct ccgacggcga agaaatgtcc atgacctccc
1981 tcaggatgcc tggctgcgct gggtgctggc tggggcgctg tgtgccggtg gctgggcagt
2041 gaactacctc ccgttcttcc tgatggagaa gacactcttc ctctaccact acctgcccgc
2101 actcaccttc caaatccttc tgctccctgt ggtcctgcag cacatcagcg accacctgtg
2161 caggtcccag ctccagagga gcatcttcag cgccctggtg gtggcctggt actcctccgc
2221 gtgccacgtg tccaacacgc tgcgcccact cacctacggg gacaagtcac tctcgccaca
2281 tgaactcaag gcccttcgct ggaaagacag ctgggacatc ttgatccgaa aacactagaa
2341 caagagtgtg gcaaagaaca cccgtgctgg ggtcgggacg aggttgaagg gtcttggtca
2401 atgtacgtaa tgagcagggt gggccccacg ctgggaggac acgggctggg ctgagcaggg
2461 cctctagtgg aacacatggg ggtctcattg aaaagctctc tgatgagcac ctccttttgt
2521 gcaaagttaa ttttttctcg acaataaaga tattccgtgt cttcacccct g
//