LOCUS AK074855 3024 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90374 fis, clone NT2RP2004648, weakly similar
to Mouse beta-galactosidase (BGAL) gene.
ACCESSION AK074855
VERSION AK074855.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3024)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..3024
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2004648"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081890"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 59..1969
/codon_start=1
/protein_id="BAC11247.1"
/translation="MTTWSLRRRPARTLGLLLLVVLGFLVLRRLDWSTLVPLRLRHRQ
LGLQAKGWNFMLEDSTFWIFGGSIHYFRVPREYWRDRLLKMKACGLNTLTTYVPWNLH
EPERGKFDFSGNLDLEAFVLMAAEIGLWVILRPGPYICSEMDLGGLPSWLLQDPGMRL
RTTYKGFTEAVDLYFDHLMSRVVPLQYKRGGPIIAVQVENEYGSYNKDPAYMPYVKKA
LEDRGIVELLLTSDNKDGLSKGIVQGVLATINLQSTHELQLLTTFLFNVQGTQPKMVM
EYWTGWFDSWGGPHNILDSSEVLKTVSAIVDAGSSINLYMFHGGTNFGFMNGAMHFHD
YKSDVTSYDYDAVLTEAGDYTAKYMKLRDFFGSISGIPLPPPPDLLPKMPYEPLTPVL
YLSLWDALKYLGEPIKSEKPINMENLPVNGGNGQSFGYILYETSITSSGILSGHVRDR
GQVFVNTVSIGFLDYKTTKIAVPLIQGYTVLRILVENRGRVNYGENIDDQRKGLIGNL
YLNDSPLKNFRIYSLDMKKSFFQRFGLDKWSSLPETPTLPAFFLGSLSISSTPCDTFL
KLEGWEKGVVFINGQNLGRYWNIGPQKTLYLPGPWLSSGINQVIVFEETMAGPALQFT
ETPHLGRNQYIK"
BASE COUNT 642 a 865 c 845 g 672 t
ORIGIN
1 gcgcggctga gtgcggactg gagtgggaac ccgggtcccc gcgcttagag aacacgcgat
61 gaccacgtgg agcctccggc ggaggccggc ccgcacgctg ggactcctgc tgctggtcgt
121 cttgggcttc ctggtgctcc gcaggctgga ctggagcacc ctggtccctc tgcggctccg
181 ccatcgtcag ctggggctgc aggccaaggg ctggaacttc atgctggagg attccacctt
241 ctggatcttc gggggctcca tccactattt ccgtgtgccc agggagtact ggagggaccg
301 cctgctgaag atgaaggcct gtggcttgaa caccctcacc acctatgttc cgtggaacct
361 gcatgagcca gaaagaggca aatttgactt ctctgggaac ctggacctgg aggccttcgt
421 cctgatggcc gcagagatcg ggctgtgggt gattctgcgt ccaggcccct acatctgcag
481 tgagatggac ctcgggggct tgcccagctg gctactccaa gaccctggca tgaggctgag
541 gacaacttac aagggcttca ccgaagcagt ggacctttat tttgaccacc tgatgtccag
601 ggtggtgcca ctccagtaca agcgtggggg acctatcatt gccgtgcagg tggagaatga
661 atatggttcc tataataaag accccgcata catgccctac gtcaagaagg cactggagga
721 ccgtggcatt gtggaactgc tcctgacttc agacaacaag gatgggctga gcaaggggat
781 tgtccaggga gtcttggcca ccatcaactt gcagtcaaca cacgagctgc agctactgac
841 cacctttctc ttcaacgtcc aggggactca gcccaagatg gtgatggagt actggacggg
901 gtggtttgac tcgtggggag gccctcacaa tatcttggat tcttctgagg ttttgaaaac
961 cgtgtctgcc attgtggacg ccggctcctc catcaacctc tacatgttcc acggaggcac
1021 caactttggc ttcatgaatg gagccatgca cttccatgac tacaagtcag atgtcaccag
1081 ctatgactat gatgctgtgc tgacagaagc cggcgattac acggccaagt acatgaagct
1141 tcgagacttc ttcggctcca tctcaggcat ccctctccct cccccacctg accttcttcc
1201 caagatgccg tatgagccct taacgccagt cttgtacctg tctctgtggg acgccctcaa
1261 gtacctgggg gagccaatca agtctgaaaa gcccatcaac atggagaacc tgccagtcaa
1321 tgggggaaat ggacagtcct tcgggtacat tctctatgag accagcatca cctcgtctgg
1381 catcctcagt ggccacgtgc gtgatcgggg gcaggtgttt gtgaacacag tatccatagg
1441 attcttggac tacaagacaa cgaagattgc tgtccccctg atccagggtt acaccgtgct
1501 gaggatcttg gtggagaatc gtgggcgagt caactatggg gagaatattg atgaccagcg
1561 caaaggctta attggaaatc tctatctgaa tgattcaccc ctgaaaaact tcagaatcta
1621 tagcctggat atgaagaaga gcttctttca gaggttcggc ctggacaaat ggagttccct
1681 cccagaaaca cccacattac ctgctttctt cttgggtagc ttgtccatca gctccacccc
1741 ttgtgacacc tttctgaagc tggagggctg ggagaagggg gttgtattca tcaatggcca
1801 gaaccttgga cgttactgga acattggacc ccagaagacg ctttacctcc caggtccctg
1861 gttgagcagc ggaatcaacc aggtcatcgt ttttgaggag acgatggcgg gccctgcatt
1921 acagttcacg gaaacccccc acctgggcag gaaccagtac attaagtgag cggtggcacc
1981 ccctcctgct ggtgccagtg ggagactgcc gcctcctctt gacctgaagc ctggtggctg
2041 ctgccccact cctcactgca aaagcatctc cttaagtagc aacctcaggg actgggggct
2101 acagtctgcc cctgtctcag ctcaaaaccc taagcctgca gggaaaggtg ggatggctct
2161 gggcctggct ttgttgatga tggctttcct acagccctgc tcttgtgccg aggctgtcgg
2221 gctgtctcta gggtgggagc agctaatcag atcgcccagc ctttggccct cagaaaaagt
2281 gctgaaacgt gcccttgcac cggacgtcac agccctgcga gcatctgctg gactcaggcg
2341 tgctctttgc tggttcctgg gaggcttggc cacatccctc atggccccat tttatccccg
2401 aaatcctggg tgtgtcacca gtgtagaggg tggggaaggg gtgtctcacc tgagctgact
2461 ttgttcttcc ttcacaacct tctgagcctt ctttgggatt ctggaaggaa ctcggcgtga
2521 gaaacatgtg acttcccctt tcccttccca ctcgctgctt cccacagggt gacaggctgg
2581 gctggagaaa cagaaatcct caccctgcgt cttcccaagt tagcaggtgt ctctggtgtt
2641 cagtgaggag gacatgtgag tcctggcaga agccatggcc catgtctgca catccaggga
2701 ggaggacaga aggcccagct cacatgtgag tcctggcaga agccatggcc catgtctgca
2761 catccaggga ggaggacaga aggcccagct cacatgtgag tcctggcaga agccatggcc
2821 catgtctgca catccaggga ggaggacaga aggcccagct cagtggcccc cgccccccac
2881 cccccacgcc cgaacagcag gggcagagca gccctccttc gaagtgtgtc caagtccgca
2941 tttgagcctt gttctggggc ccagcccaac acctggcttg ggctcactgt cctgagttgc
3001 agtaaagcta taaccttgaa tcac
//