LOCUS AK074853 2532 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90372 fis, clone NT2RP2004556.
ACCESSION AK074853
VERSION AK074853.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2532)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..2532
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2004556"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081888"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 375..938
/codon_start=1
/protein_id="BAC11246.1"
/translation="MVLLWVVTCPATMLTEPQNPHLIGFVAYSGPSHTTQPHKYWLLL
DGQADPAAAEGPVKRKAASVVWWPQALRHLSLLVHCWEESYEMNIGCQSLWAGGLASS
GNGWDLGVAFRRDTCMSSSSLHWKEFKYAPGSLHYFALSFVLILTEICLVSSGMGFPQ
EGKHFSVLGSPDCSLWGRDEHVPREFA"
BASE COUNT 564 a 590 c 640 g 738 t
ORIGIN
1 aagggacttc cgtgctcccg gcgagcgggc ggagagcggg ggccgcactg gggagtgtgg
61 gctgggccgc agatgtcatg tggcctgtgt tttggaccgt ggttcgtacc tatgctcctt
121 atgtcacatt ccctgttgcc ttcgtggtcg gggctgtggg ttaccacctg gaatggttca
181 tcaggggaaa ggacccccag cccgtggagg aggaaaagag catctcagag cgccgggagg
241 atcgcaagct ggatgagctt ctaggcaagg accacacgca ggtggtgagc cttaaggaca
301 agctagaatt tgccccgaaa gctgtgctga acagaaaccg cccagagaag aattaatgga
361 ggacacaggg ccctatggtc ctactgtggg tggtgacttg tcctgctacc atgttgacag
421 agccccagaa cccacatcta attggctttg ttgcttattc tggcccttcc cacaccacac
481 agccacacaa atactggctg ctccttgatg gccaggcaga cccagcagca gccgaggggc
541 cagtgaagag gaaggccgca tctgttgtgt ggtggccaca agcactcagg catctgagtt
601 tactggtgca ctgctgggag gagagttatg agatgaacat tggctgtcaa tctctgtggg
661 caggcggttt ggcctctagt gggaatggct gggatttggg cgttgccttt aggagggata
721 cctgcatgtc tagttccagt ctgcactgga aagaattcaa atatgcacct ggctcccttc
781 actattttgc cctatccttt gtgctcattc ttactgaaat ctgtcttgtc agctcaggaa
841 tgggattccc ccaggaagga aagcactttt ctgttctggg aagcccagac tgttcacttt
901 ggggcaggga cgaacatgtg cctcgtgaat ttgcttgaaa acagtcacca tcttctaccc
961 ccatcactgt atagtgaaaa acctgattaa agtggtatct gagaaccata atgatcgaat
1021 cttatcatcc aggtggtatt tgggggtctt cttaggagtt aggcaagtac aggcatttta
1081 gagcaggata acattggtat taaagggtgc caaggagccc tggaaactgc tttactagtg
1141 cggttgcact gaaccctgga ggcacagatt cccaggaacc tttttaccat tattacaatt
1201 ggctgcagca ccttcaagtc taaatggagg tagacctcac atacacacac atagacgcct
1261 ttccctataa aagtatatac gcacattcaa gaaaatttgg tagggatttt aaaaaggtca
1321 cccatgttcc cagctcgtta ataacaaccg ttaacatttt gttgtctttt actaattctc
1381 tgcataggtt ttagagatag ttatcattac tgtacattta attttatata cttttctcac
1441 tattatcatt ttcccatttt acatggtcat cataattggt tttttggaga cagggtctca
1501 ctctgtcaca caggctggag tgcagtacaa ttacggctca gtgcaacctt gacctcctgg
1561 gctcaagtga tcctctcact tcagctcctc aagtagatgg gactacagga gtatgccacc
1621 atgcccagct aattttttta tttttttgta gaaccaggtc tcactatgtt gcccaggcta
1681 gtctcaaact cctgggctca agtgatcctc cggccttggc ctgtagtttc ttttttcttt
1741 ttttttggag atggactctc attctgtcac ccgggctgga gtgcagtggc gtgatcttgg
1801 ctgactgcaa cttctgcctc ctgggttcaa gcgattctcc tacctcagct tcctaagtag
1861 ctgggattac aggcgtgcgc catcgtgcct ggctagtttt tgtgttttta gtaaagacag
1921 gggtttcacc atcttggcca ggctggtctc gaactcctga cctcaagcga tcccatctgc
1981 ctcggcctcc caaagtgctg ggattacagg tgtgaggcac cgtgcctggc agtctgtagt
2041 ttttttttgt tgttgttgtt gttgttgttt taaatatgga gtctcgctgt attgcccagg
2101 ctggagtgca gtggcgcgat cttggttcac tgcaacctcc acctcttggg ttcaagcgat
2161 tctcctgcct cagcctcctg agtagctggg attacaggtg tgcgccgcta cgcctggcta
2221 atttttgtat ttttagtaga gacagggttt caccatgttg gtcaggctgg tctcaaactt
2281 ctgaccttgt gatctgcctg ccttggcctc ctaaagtgct gggattacag gcgtgagcca
2341 ccgtgcctgg ctgtagttgt ttataatagc aacataattg tctatcaagt gggagtatga
2401 cataagttta tcctggacaa tttccatatt gctggacatt tgaattgttt ctggttggtc
2461 actcgtttag atactgcaac gaatatctta tatgaagctt ttcctgcgtt ttggaataaa
2521 cagtaaaaat ag
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