LOCUS AK074819 2080 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90338 fis, clone NT2RP2002527, highly similar
to Fatty acid desaturase 1.
ACCESSION AK074819
VERSION AK074819.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2080)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..2080
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2002527"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081854"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 63..1568
/codon_start=1
/protein_id="BAC11229.1"
/translation="MGTRAARPAGLPCGAENPARRRLALGARQQIHSWSPRTPSTRLT
APAGPARGVARPAMAPDPVAAETAAQGPTPRYFTWDEVAQRSGCEERWLVIDRKVYNI
SEFTRRHPGGSRVISHYAGQDATDPFVAFHINKGLVKKYMNSLLIGELSPEQPSFEPT
KNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGTSFLPFL
LCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMHFQH
HAKPNCFRKDPDINMHPFFFALGKILSVELGKQKKKYMPYNHQHKYFFLIGPPALLPL
YFQWYIFYFVIQRKKWVDLAWMITFYVRFFLTYVPLLGLKAFLGLFFIVRFLESNWFV
WVTQMNHIPMHIDHDRNMDWVSTQLQATCNVHKSAFNDWFSGHLNSQIEHHLFPTMPR
HNYHKVAPLVQSLCAKHGIEYQSKPLLSAFADIIHSLEESGQLWLDAYLHQ"
BASE COUNT 453 a 607 c 576 g 444 t
ORIGIN
1 gtgagttgcc caagacccac cgggggacgg gatctcgctc cccgcgccac gaggctcggc
61 caatgggaac gcgcgctgcg aggcccgccg gtctgccctg cggtgctgaa aacccggcgc
121 gcaggcggct ggctctgggc gcgcgccagc aaatccactc ctggagcccg cggaccccga
181 gcacgcgcct gacagcccct gctggcccgg cgcgcggcgt cgccaggcca gctatggccc
241 ccgacccggt ggccgccgag accgcggctc agggacctac cccgcgctac ttcacctggg
301 acgaggtggc ccagcgctca gggtgcgagg agcggtggct agtgatcgac cgtaaggtgt
361 acaacatcag cgagttcacc cgccggcatc cagggggctc ccgggtcatc agccactacg
421 ccgggcagga tgccacggat ccctttgtgg ccttccacat caacaagggc cttgtgaaga
481 agtatatgaa ctctctcctg attggagaac tgtctccaga gcagcccagc tttgagccca
541 ccaagaataa agagctgaca gatgagttcc gggagctgcg ggccacagtg gagcggatgg
601 ggctcatgaa ggccaaccat gtcttcttcc tgctgtacct gctgcacatc ttgctgctgg
661 atggtgcagc ctggctcacc ctttgggtct ttgggacgtc ctttttgccc ttcctcctct
721 gtgcggtgct gctcagtgca gttcaggccc aggctggctg gctgcagcat gactttgggc
781 acctgtcggt cttcagcacc tcaaagtgga accatctgct acatcatttt gtgattggcc
841 acctgaaggg ggcccccgcc agttggtgga accacatgca cttccagcac catgccaagc
901 ccaactgctt ccgcaaagac ccagacatca acatgcatcc cttcttcttt gccttgggga
961 agatcctctc tgtggagctt gggaaacaga agaaaaaata tatgccgtac aaccaccagc
1021 acaaatactt cttcctaatt gggcccccag ccttgctgcc tctctacttc cagtggtata
1081 ttttctattt tgttatccag cgaaagaagt gggtggactt ggcctggatg attaccttct
1141 acgtccgctt cttcctcact tatgtgccac tattggggct gaaagccttc ctgggccttt
1201 tcttcatagt caggttcctg gaaagcaact ggtttgtgtg ggtgacacag atgaaccata
1261 ttcccatgca cattgatcat gaccggaaca tggactgggt ttccacccag ctccaggcca
1321 catgcaatgt ccacaagtct gccttcaatg actggttcag tggacacctc aactcccaga
1381 ttgagcacca tctttttccc acgatgcctc gacacaatta ccacaaagtg gctcccctgg
1441 tgcagtcctt gtgtgccaag catggcatag agtaccagtc caagcccctg ctgtcagcct
1501 tcgccgacat catccactca ctagaggagt cagggcagct ctggctagat gcctatcttc
1561 accaataaca acagccaccc tgcccagtct ggaagaagag gaggaagact ctggagccaa
1621 ggcagagggg agcttgaggg acaatgccac tatagtttaa tactcagagg gggttgggtt
1681 tggggacata aagcctctga ctcaaactcc tcccttttat cttctagcca cagttctaag
1741 acccaaagtg gggggtggac acagaagtcc ctaggaggga aggagctgtt ggggcagggg
1801 tgtaaattat ttcctttttc tagtttggca catgcaggta gttggtgaac agagagaacc
1861 aggagggtaa cagaagagga gggacctact gaacccagag tcaggaagag atttaacact
1921 aaaattccac tcatgccggg cgtggtggca cgcgcctgta atcccagcta cccaggaggc
1981 tgaggcagga gaatcgcttg aaccggggag gtggaggttg cagtgagctg agatcacgcc
2041 attgtactcc agcctgggcg acagagcaag actccatttc
//