LOCUS AK074803 4755 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90322 fis, clone NT2RP2001755, highly similar
to Rattus norvegicus f-spondin mRNA.
ACCESSION AK074803
VERSION AK074803.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4755)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..4755
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2001755"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081838"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 215..2638
/codon_start=1
/protein_id="BAC11217.1"
/translation="MRLSPAPLKLSRTPALLALALPLAAALAFSDETLDKVPKSEGYC
SRILRAQGTRREGYTEFSLRVEGDPDFYKPGTSYRVTLSAAPPSYFRGFTLIALRENR
EGDKEEDHAGTFQIIDEEETQFMSNCPVAVTESTPRRRTRIQVFWIAPPAGTGCVILK
ASIVQKRIIYFQDEGSLTKKLCEQDSTFDGVTDKPILDCCACGTAKYRLTFYGNWSEK
THPKDYPRRANHWSAIIGGSHSKNYVLWEYGGYASEGVKQVAELGSPVKMEEEIRQQS
DEVLTVIKAKAQWPAWQPLNVRAAPSAEFSVDRTRHLMSFLTMMGPSPDWNVGLSAED
LCTKECGWVQKVVQDLIPWDAGTDSGVTYESPNKPTIPQEKIRPLTSLDHPQSPFYDP
EGGSITQVARVVIERIARKGEQCNIVPDNVDDIVADLAPEEKDEDDTPETCIYSNWSP
WSACSSSTCDKGKRMRQRMLKAQLDLSVPCPDTQDFQPCMGPGCSDEDGPTCTMSEWI
TWSPCSISCGMGMRSRERYVKQFPEDGSVCTLPTEETEKCTVNEECSPSSCLMTEWGE
WDEYSATCGMGMKKRHRMIKMNPADGSMCKAETSQAEKRMMPECHTIPCLLSPWSEWS
DCSVTCGKGMRTRQRMLKSLAELGDCNEDLEQVEKCMLPECPIDCELTEWSQWSECNK
SCGKGHVIRTRMIQMEPQFGGAPCPETVQRKKCRIRKCLRNPSIQKLRWREARESRRS
EQLKEESEGEQFPGCRMRPWTAWSECTKLCGGGIQERYMTVKKRFKSSQFTSCKDKKE
IRACNVHPC"
BASE COUNT 1256 a 1182 c 1171 g 1146 t
ORIGIN
1 gctgctctcg ttcgcttggc tcagctcagc tcagctcagc gcagctccgc ggccgccaag
61 ccgaggcggg cacggtctcc gagtcgcgga cgccagctcc gagctccctc tctccgccgc
121 gcctccgcca ggtcgcgcct tcgtcgggac cacttcgggc aggagtcgcg tggcgaaggc
181 ctgcggccgc ggcacaaagt tgggggccgc gaagatgagg ctgtccccgg cgcccctgaa
241 gctgagccgg actccggcac tgctggccct ggcgctgccc ctggccgcgg cgctggcctt
301 ctccgacgag accctggaca aagtgcccaa gtcagagggc tactgcagcc gtatcctgcg
361 cgcccagggc acgcggcgcg agggctacac cgagttcagc ctccgcgtgg agggcgaccc
421 cgacttctac aagccgggaa ccagctaccg cgtaacactt tcagctgctc ctccctccta
481 cttcagagga ttcacattaa ttgccctcag agagaacaga gagggtgata aggaagaaga
541 ccatgctggg accttccaga tcatagacga agaagaaact cagtttatga gcaattgccc
601 tgttgcagtc actgaaagca ctccacggag gaggacccgg atccaggtgt tttggatagc
661 accaccagcg ggaacaggct gcgtgattct gaaggccagc atcgtacaaa aacgcattat
721 ttattttcaa gatgagggct ctctgaccaa gaaactttgt gaacaagatt ccacatttga
781 tggggtgact gacaaaccca tcttagactg ctgtgcctgc ggaactgcca agtacagact
841 cacattttat gggaattggt ccgagaagac acacccaaag gattaccctc gtcgggccaa
901 ccactggtct gcgatcatcg gaggatccca ctccaagaat tatgtactgt gggaatatgg
961 aggatatgcc agcgaaggcg tcaaacaagt tgcagaattg ggctcacccg tgaaaatgga
1021 ggaagaaatt cgacaacaga gtgatgaggt cctcaccgtc atcaaagcca aagcccagtg
1081 gccagcctgg cagcctctca acgtgagagc agcaccttca gctgaatttt ccgtggacag
1141 aacgcgccat ttaatgtcct tcctgaccat gatgggccct agtcccgact ggaacgtagg
1201 cttatctgca gaagatctgt gcaccaagga atgtggctgg gtccagaagg tggtgcaaga
1261 cctgattccc tgggacgctg gcaccgacag cggggtgacc tatgagtcac ccaacaaacc
1321 caccattccc caggagaaaa tccggcccct gaccagcctg gaccatcctc agagtccttt
1381 ctatgaccca gagggtgggt ccatcactca agtagccaga gttgtcatcg agagaatcgc
1441 acggaagggt gaacaatgca atattgtacc tgacaatgtc gatgatattg tagctgacct
1501 ggctccagaa gagaaagatg aagatgacac ccctgaaacc tgcatctact ccaactggtc
1561 cccatggtcc gcctgcagct cctccacctg tgacaaaggc aagaggatgc gacagcgcat
1621 gctgaaagca cagctggacc tcagcgtccc ctgccctgac acccaggact tccagccctg
1681 catgggccct ggctgcagtg acgaagacgg ccccacctgc accatgtccg agtggatcac
1741 ctggtcgccc tgcagcatct cctgcggcat gggcatgagg tcccgggaga ggtatgtgaa
1801 gcagttcccg gaggacggct ccgtgtgcac gctgcccact gaggaaacgg agaagtgcac
1861 ggtcaacgag gagtgctctc ccagcagctg cctgatgacc gagtggggcg agtgggacga
1921 gtacagcgcc acctgcggca tgggcatgaa gaagcggcac cgcatgatca agatgaaccc
1981 cgcagatggc tccatgtgca aagccgagac atcacaggca gagaagcgca tgatgccaga
2041 gtgccacacc atcccatgct tgctgtcccc atggtccgag tggagtgact gcagcgtgac
2101 ctgcgggaag ggcatgcgaa cccgacagcg gatgctcaag tctctggcag aacttggaga
2161 ctgcaatgag gatctggagc aggtggagaa gtgcatgctc cctgaatgcc ccattgactg
2221 tgagctcacc gagtggtccc agtggtcgga atgtaacaag tcatgtggga aaggccacgt
2281 gattcgaacc cggatgatcc aaatggagcc tcagtttgga ggtgcaccct gcccagagac
2341 tgtgcagcga aaaaagtgcc gcatccgaaa atgccttcga aatccatcca tccaaaagct
2401 acgctggagg gaggcccgag agagccggcg gagtgagcag ctgaaggaag agtctgaagg
2461 ggagcagttc ccaggttgta ggatgcgccc atggacggcc tggtcagaat gcaccaaact
2521 gtgcggaggt ggaattcagg aacgttacat gactgtaaag aagagattca aaagctccca
2581 gtttaccagc tgcaaagaca agaaggagat cagagcatgc aatgttcatc cttgttagca
2641 agggtacgag ttccccaggg ctgcactcta gattccagag tcaccaatgg ctggattatt
2701 tgcttgttta agacaattta aattgtgtac gctagttttc atttttgcag tgtggttcgc
2761 ccagtagtct tgtggatgcc agagacatcc tttctgaata cttcttgatg ggtacaggct
2821 gagtggggcg ccctcacctc cagccagcct cttcctgcag aggagtagtg tcagccacct
2881 tgtactaagc tgaaacatgt ccctctggag cttccacctg gccagggagg acggagactt
2941 tgacctactc cacatggaga ggcaaccatg tctggaagtg actatgcctg agtcccaggg
3001 tgcggcaggt aggaaacatt cacagatgaa gacagcagat tccccacatt ctcatctttg
3061 gcctgttcaa tgaaaccatt gtttgcccat ctcttcttag tggaacttta ggtctctttt
3121 caagtctcct cagtcatcaa tagttcctgg ggaaaaacag agctggtaga cttgaagagg
3181 agcattgatg ttgggtggct tttgttcttt cactgagaaa ttcggaatac atttgtctca
3241 cccctgatat tggttcctga tgccccccca acaaaaataa ataaataaat tatggctgct
3301 ttatttaaat ataaggtagc tagtttttac acctgagata aataataagc ttagagtgta
3361 tttttccctt gcttttgggg gttcagagga gtatgtacaa ttcttctggg aagccagcct
3421 tctgaacttt ttggtactaa atccttattg gaaccaagac aaaggaagca aaattggtct
3481 ctttagagac caatttgcct aaattttaaa atcttcctac acacatctag acgttcaagt
3541 ttgcaaatca gtttttagca agaaaacatt tttgctatac aaacattttg ctaagtctgc
3601 ccaaagcccc cccaatgcat tccttcaaca aaatacaatc tctgtacttt aaagttattt
3661 tagtcatgaa attttatatg cagagagaaa aagttaccga gacagaaaac aaatctaagg
3721 gaaaggaata ttatgggatt aagctgagca agcaattctg gtggaaagtc aaacctgtca
3781 gtgctccaca ccagggctgt ggtcctccca gacatgcata ggaatggcca caggtttaca
3841 ctgccttccc agcaattata agcacaccag attcagggag actgaccacc aagggatagt
3901 gtaaaaggac attttctcag ttgggtccat cagcagtttt tcttcctgca tttattgttg
3961 aaaactattg tttcatttct tcttttatag gccttattac tgcttaatcc aaatgtgtac
4021 cattggtgag acacatacaa tgctctgaat acactacgaa tttgtattaa acacatcaga
4081 atatttccaa atacaacata gtatagtcct gaatatgtac ttttaacaca agagagacta
4141 ttcaataaaa actcactggg tctttcatgt ctttaagcta agtaagtgtt cagaaggttc
4201 ttttttatat tgtcctccac ctccatcatt ttcaataaaa gatagggctt ttgctccctt
4261 gttcttggag ggaccattat tacatctctg aactaccttt gtatccaaca tgttttaaat
4321 ccttaaatga attgctttct cccaaaaaaa gcacagtata aagaaacaca agatttaatt
4381 atttttctac ttggggggaa aaaagtcctc atgtagaagc acccactttt gcaatgttgt
4441 tctaagctat ctatctaact ctcagcccat gataaagttc cttaagctgg tgattcctaa
4501 tcaaggacaa gccaccctag tgtctcatgt ttgtatttgg tcccagttgg gtacatttta
4561 aaatcctgat tttggagact taaaaccagg ttaatggcta agaatgggta acatgactct
4621 tgttggattg ttattttttg tttgcaatgg ggaatttata agaagcatca agtctctttc
4681 ttaccaaagt cttgttaggt ggtttatagt tcttttggct aacaaatcat tttggaaata
4741 aagatttttt actac
//