LOCUS AK074780 2828 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90299 fis, clone NT2RP2000514, highly similar
to Homo sapiens roundabout 2 (robo2) mRNA.
ACCESSION AK074780
VERSION AK074780.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2828)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..2828
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2000514"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081815"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 131..1843
/codon_start=1
/protein_id="BAC11205.1"
/translation="MSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQ
IVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNI
QRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKC
KATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGET
SWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAY
IIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPV
RTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVM
YRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTV
RTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFH
INKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPNNTGYPICHDTDLAF
QQHT"
BASE COUNT 888 a 659 c 615 g 666 t
ORIGIN
1 agctgtagaa tttcgttgtc aagtccaagg agatcctcaa ccaactgtga ggtggaaaaa
61 ggatgatgca gacttgccaa gaggaaggta tgacatcaaa gacgattaca cactaagaat
121 taaaaagacc atgagtacag atgaaggcac ctatatgtgt attgctgaga atcgggttgg
181 aaaaatggaa gcctctgcta cactcaccgt ccgagctcgc cctgttgctc ccccacagtt
241 tgtggttcgg ccaagagatc agattgttgc tcaaggtcga acagtgacat ttccctgtga
301 aactaaagga aacccacagc cagctgtttt ttggcagaaa gaaggcagcc agaacctact
361 tttcccaaac caaccccagc agcccaacag tagatgctca gtgtcaccaa ctggagacct
421 cacaatcacc aacattcaac gttccgacgc gggttactac atctgccagg ctttaactgt
481 ggcaggaagc attttagcaa aagctcaact ggaggttact gatgttttga cagatagacc
541 tccacctata attctacaag gcccagccaa ccaaacgctg gcagtggatg gtacagcgtt
601 actgaaatgt aaagccactg gtgatcctct tcctgtaatt agctggttaa aggagggatt
661 tacttttccg ggtagagatc caagagcaac aattcaagag caaggcacac tgcagattaa
721 gaatttacgg atttctgata ctggcactta tacttgtgtg gctacaagtt caagtggaga
781 gacttcctgg agtgcagtgc tggatgtgac agagtctgga gcaacaatca gtaaaaacta
841 tgatttaagt gacctgccag ggccaccatc caaaccgcag gtcactgatg ttactaagaa
901 cagtgtcacc ttgtcctggc agccaggtac ccctggaacc cttccagcaa gtgcatatat
961 cattgaggct ttcagccaat cagtgagcaa cagctggcag accgtggcaa accatgtaaa
1021 gaccaccctc tatactgtaa gaggactgcg gcccaataca atctacttat tcatggtcag
1081 agcgatcaac ccccaaggtc tcagtgaccc aagtcccatg tcagatcctg tgcgcacaca
1141 agatatcagc ccaccagcac aaggagtgga ccacaggcaa gtgcagaaag agctaggaga
1201 tgtccttgtc cgtcttcata atccagttgt gctgactccc accacggttc aggtcacatg
1261 gacggttgat cgccaacccc agtttatcca aggctaccga gtgatgtatc gtcagacttc
1321 aggtctgcag gcgacatctt cgtggcagaa tttagatgcc aaagtcccga ctgaacgaag
1381 tgctgtctta gtcaacctga aaaagggggt gacttatgaa attaaagtac ggccatattt
1441 taatgagttc caaggaatgg atagtgaatc taaaacggtt cgtactactg aagaagcccc
1501 aagtgcccca ccacagtctg tcactgtact gacagttgga agctacaata gcacaagtat
1561 tagtgtttcc tgggatcctc ctcctccaga tcaccagaat ggaattatcc aagaatacaa
1621 gatctggtgt ctaggaaatg aaacgcgatt ccatatcaac aaaactgtgg atgcagccat
1681 tcggtccgta ataattggtg gattattccc aggtattcaa taccgggtag aggttgcagc
1741 tagtaccagt gcaggggttg gagtaaagag tgagccacag ccaaataaca caggctaccc
1801 catatgccac gacacagatc ttgcattcca acagcataca tgaattggct gtcgatctgc
1861 ctgatccaca atggaaaagc tcaattcagc aaaaaacaga tctgatggga tttggttatt
1921 ctctacctga tcagaacaaa ggtaacaatg gtgggaaagg tggaaaaaag aagaaaaata
1981 aaaactcttc taaaccacag aaaaacaatg gatccacttg ggccaatgtc cctctacctc
2041 ccccccccag tccagcccct tcctggcacg gagctggaac actatgcagt ggaacaacaa
2101 gaaaatggct atccatggct tgctgattct tggccagcca cgagcttgcc agtaaataat
2161 agcaacagtg gcccaaatga gattggaaat tttggccgtg gagatgtgct gccaccagtt
2221 ccaggccaag gggataaaac agcaacgatg ctctcagatg gagccattta tagtagcatt
2281 gacttcacta ccaaaaccag ttacaacagt tccagccaaa taacacaggc taccccatat
2341 gccacgggaa gagatgcaac ccatgctgca ggctcacctg gatgagttga caagagccta
2401 tcagtttgat atagcaaaac aaacatggta aatatcttca ttaagtcaag agacccatgc
2461 tttcaacaca tggaaatttt tttcagaata aattcacaag agattctgat agaactacac
2521 agctagggtt aaaagtgatt ttgtataact ccttaatcat ttagataaat gtatccactg
2581 agaaatgtta aataatttgg ccaaaactag aactagaact agaactagaa ctggaactgg
2641 agctgtaact gtaactgtaa ctagaactag aactaaaact agaactagag caagaattca
2701 aatctgctgt atttcaggct aaagtgctct tggctacgcc agaatctttt ctaatttatg
2761 gtttatcttc aattagctta tcattgaact cacatcccat gatgtaataa aagactcctt
2821 ttagattc
//