LOCUS AK074779 2627 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90298 fis, clone NT2RP2000479.
ACCESSION AK074779
VERSION AK074779.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2627)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..2627
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2000479"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000081814"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 121..519
/codon_start=1
/protein_id="BAC11204.1"
/translation="MTLAARAMRNNFRRYFIEPSQLKLFYDVITWIVTQVAISYTVVP
FVLLSIKPSLTFYSSWYYCLHILGILVLLLLPVKKTQRRKNTHENIQLSQSKKFDEGE
NSLGQNSFSTTNNVCNQNQEIASRHSSLKQ"
BASE COUNT 780 a 478 c 582 g 787 t
ORIGIN
1 ctcaaaaggg tgtgttatga acgaacctcc ttcagtccaa ctatccagac gttcattctc
61 tctgccattt ggcacggggt atacccagga tattatctaa cgtttctaac aggggtgtta
121 atgacattag cagcaagagc tatgagaaat aactttagac gttatttcat tgaaccttcc
181 caactgaaat tattttatga tgttataaca tggatagtaa ctcaagtagc aataagttac
241 acagttgtgc catttgtgct tctttctata aaaccatcac tcacgtttta cagctcctgg
301 tattattgcc tgcacattct tggtatctta gtattattgt tgttgccagt gaaaaaaact
361 caaagaagaa agaatacaca tgaaaacatt cagctctcac aatccaaaaa gtttgatgaa
421 ggagaaaatt ctttgggaca gaacagtttt tctacaacaa acaatgtttg caatcagaat
481 caagaaatag cctcgagaca ttcatcacta aagcagtgat cgggaaggct ctgagggctg
541 tttttttttt ttgatgttaa cagaaaccaa tcttagcacc ttttcaaggg gtttgagttt
601 gttggaaaag cagttaactg gggggaaatg gacagttata gataaggaat ttcctgtaca
661 ccagattgga aatggagtga aacaagccct cccatgccat gtccccgtgg gccacgcctt
721 atgtaagaat atttccatat ttcagtgggc actcccaacc tcagcacttg tccgtagggt
781 cacacgcgtg ccctgttgct gaatgtatgt tgcgtatccc aaggcactga agaggtggaa
841 aaataatcgt gtcaatctgg atgatagaga gaaattaact tttccaaatg aatgtcttgc
901 cttaaaccct ctatttccta aaatattgtt cctaaatggt attttcaagt gtaatattgt
961 gagaacgcta ctgcagtagt tgatgttgtg tgctgtaaag gattttagga ggaatttgaa
1021 acaggatatt taagagtgtg gatattttta aaatgcaata aacatctcag tatttgaagg
1081 gttttcttaa agtatgtcaa atgactacaa tccatagtga aactgtaaac agtaatggac
1141 gccaaattat aggtagctga ttttgctgga gagtttaatt accttgtgca gtcaaagagc
1201 gcttccagaa ggaatctctt aaaacataat gagaggtttg gtaatgtgat attttaagct
1261 tattcttttt cttaaaagag agaggtgacg aaggaaggca ggaatgaaga agcactgcgt
1321 ggcctccggt ggaatgcacg gggcacagcc gcgactctgc aggcagcttc ccccccatgc
1381 cagggctctg cgccgtcatg tgagacttaa aaaaaaagtt gaatgacttc gtgatacttt
1441 ggacttctaa attaaattta tcaggcataa attatgtaga attagaggct ttgaaaataa
1501 tactggtagg ttgctcaaag gttttgaaag agaaatcgct aggtaggtta ctatctggct
1561 aatccatttc ttatccttga caatttaatt catatttggg aaacttttag ggaaatgaaa
1621 aataaaagtc actgagtctg ggtgacattt tttaagaata atataaattc agtttcaaac
1681 tcttctcaca ttaaaatttt gctgtgaact cttactaaaa tgagttttag gttctgtaag
1741 tggaaaaatg tgcttttatt ttatgggcca tttttaccac aactaatctt gccttggatt
1801 actaagcatc tcctgcgatc ccacagagga ctgtggtggc cacaggagct gaaagcagaa
1861 gagtgggatt tgatgccagg cagtggagtg gcctcagccc cagattgtac ctcctgccct
1921 gtaggagggg agggggcaaa gccttctgac ttcacctttg tttgacctat gtatggaact
1981 tacttttact ttttgcctta aatttttaat gaaatgcaaa ttttctgtga tggggttctc
2041 tctctctttt tttcgggggg tggagtcact aataaatttg caaatgaagt taaagacaag
2101 gcaaccatct ggcttatgct atataatact tcatttaaag aagaaaggaa aagcaaatgc
2161 acttgcagct tttgaggtct cagcaaaaat gggcatgtgt cttttttgaa gtttagaaat
2221 atcctaatct atttttattt atctaaaagt aagtgttttc cggctgataa ggctaaccct
2281 acccaggaaa ggattgataa ctaaataaat ttcctctgtt ttcccatgca ttgaaattat
2341 gttggctgag catggtggct cacacctgta atcctagcac tttgggaggc cgaggtgggc
2401 ggatcacttg aggtcaggag ttggagacca gcctggccaa cgtggtgaat ccccgtctct
2461 actgaaaaca caaaaattag acgggcatgg tggcgcacac ctgtaatccc agctacttgg
2521 gaggctgagg caggagaatt gcttgaacct gggaggtgga ggttgcagtg agctaaaatt
2581 gtgccactgc actccagcct gggtgacaga ggaagactcc gtctcac
//