LOCUS AK074760 1656 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90279 fis, clone NT2RP1000448.
ACCESSION AK074760
VERSION AK074760.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1656)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..1656
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP1000448"
/clone_lib="NT2RP1"
/db_xref="H-InvDB:HIT000081795"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pUC19FL3"
/note="mRNA from NT2 neuronal precursor cells after
48-hours retinoic acid (RA) induction"
/organism="Homo sapiens"
CDS 136..687
/codon_start=1
/protein_id="BAC11188.1"
/translation="MASRAGPRTAGTDGSDFQHRERVAMHYQMSVTLKYEIKKLIYVH
LVIWLLLVAKMSVGHLRLLSHDQVAMPYQWEYPYLLSILPSLLGLLSFPRNNISYLVL
SMISMGLFSIAPLIYGSMEMFPAAQQLYRHGKAYRFLFGFSAVSIMYLVLVLAVQVHA
WQLYYSKKLLDSWFTSTQEKKHK"
BASE COUNT 407 a 378 c 415 g 456 t
ORIGIN
1 agagacagag gggccggaag ttctcttcac ggagccgcgc ggctgcgggg gcgcaaatag
61 ggtcactggg ccgcttggcg gtgtcgttgc ggtaccaggt ccgcgtgagg ggttcggggg
121 ttctgggcag gcacaatggc gtctcgagca ggcccgcgaa cggccggcac cgacggcagc
181 gactttcagc accgggagcg cgtcgccatg cactaccaga tgagtgtgac cctcaagtat
241 gaaatcaaga agctgatcta cgtacatctg gtcatatggc tgctgctggt tgctaagatg
301 agcgtgggac acctgaggct cttgtcacat gatcaggtgg ccatgcccta tcagtgggaa
361 tacccgtatt tgctgagcat tttgccctct ctcttgggcc ttctctcctt tccccgcaac
421 aacattagct acctggtgct ctccatgatc agcatgggac tcttttccat cgctccactc
481 atttatggca gcatggagat gttccctgct gcacagcagc tctaccgcca tggcaaggcc
541 taccgtttcc tctttggttt ttctgccgtt tccatcatgt acctggtgtt ggtgttggca
601 gtgcaagtgc atgcctggca gttgtactac agcaagaagc tcctagactc ttggttcacc
661 agcacacagg agaagaagca taaatgaagc ctctttgggg tgaagcctgg acatcccatc
721 gaatgaaagg acactagtac agcggttcca aaatcccttc tggtgatttt agcagctgta
781 atgttggtac ctggtgcaga ccaggccaaa gttctggaaa gctccttttg ccatctgctg
841 aggtggcaaa actataattt attcctggtt ggccagaact gggtgaccaa cagctatgaa
901 acaaatttca gctgtttgaa gttgaacttt gaggtttttt ctttaagaat gagcttcgtc
961 cttgcctcta ctcggtcatt ctccccattt ccatccatta ccccttagcc attgagacta
1021 aaggaaatag ggaataaatc aaattacttc atctctaggt cacgggtcag gaaacatttg
1081 ggcagctgct cccttggcag gctgtggtct cctctgcaaa gcattttaat taaaaacctc
1141 aataaagatg gccctgccca cactgtgcct tgtgtttggg gcttgataga gtgtgaaagg
1201 tgtgttgctt gaaaggtgtg gccttagaca ctaggagatg atgggtagaa gctccttgag
1261 aaacctagca gtggccatag agaagtagac tgggtatggg gaacctaagt catagttgta
1321 acctacagct gattggtaag aactatgggc cagacattta atctctccat ctatgaaata
1381 gtccattctc atttatctag cttaggttat gggctcttct tttagttgga tattcacatt
1441 tatctttcag tgttaactgg gtatcagtta tgagtctact actgtataat ggcatccaaa
1501 agaattttaa agtacctcgg tttataggga tttacaattc agtagaaact tgttattcat
1561 gtgaaacagt gaacataata tctaatgatg tggagcaagt acattaaaaa caggaaggaa
1621 gagatctgtg attaaactaa aggccctttc acacct
//