LOCUS AK074746 1979 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90265 fis, clone NT2RM4001325, highly similar
to Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4.
ACCESSION AK074746
VERSION AK074746.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1979)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..1979
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM4001325"
/clone_lib="NT2RM4"
/db_xref="H-InvDB:HIT000081781"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells"
/organism="Homo sapiens"
CDS 69..1229
/codon_start=1
/protein_id="BAC11177.1"
/translation="MLLPKKMKLLLFLVSQMAILALFFHMYSHNISSLSMKAQPERMH
VLVLSSWRSGSSFVGQLFGQHPDVFYLMEPAWHVWMTFKQSTAWMLHMAVRDLIRAVF
LCDMSVFDAYMEPGPRRQSSLFQWENSRALCSAPACDIIPQDEIIPRAHCRLLCSQQP
FEVVEKACRSYSHVVLKEVRFFNLQSLYPLLKDPSLNLHIVHLVRDPRAVFRSRERTK
GDLMIDNRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRY
EDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAW
RWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH"
BASE COUNT 451 a 542 c 474 g 512 t
ORIGIN
1 actttcacag cttcctggga gcgagtgctt tctcaagccc gtcttgcaag gtcttccact
61 tcagcacaat gctactgcct aaaaaaatga agctcctgct gtttctggtt tcccagatgg
121 ccatcttggc tctattcttc cacatgtaca gccacaacat cagctccctg tctatgaagg
181 cacagcccga gcgcatgcac gtgctggttc tgtcttcctg gcgctctggc tcttcttttg
241 tggggcagct ttttgggcag cacccagatg ttttctacct gatggagccc gcctggcacg
301 tgtggatgac cttcaagcag agcaccgcct ggatgctgca catggctgtg cgggatctga
361 tacgggccgt cttcttgtgc gacatgagcg tctttgatgc ctacatggaa cctggtcccc
421 ggagacagtc cagcctcttt cagtgggaga acagccgggc cctgtgttct gcacctgcct
481 gtgacatcat cccacaagat gaaatcatcc cccgggctca ctgcaggctc ctgtgcagtc
541 aacagccctt tgaggtggtg gagaaggcct gccgctccta cagccacgtg gtgctcaagg
601 aggtgcgctt cttcaacctg cagtccctct acccgctgct gaaagacccc tccctcaacc
661 tgcatatcgt gcacctggtc cgggaccccc gggccgtgtt ccgttcccga gaacgcacaa
721 agggagatct catgattgac aatcgcattg tgatggggca gcatgagcaa aaactcaaga
781 aggaggacca accctactat gtgatgcagg tcatctgcca aagccagctg gagatctaca
841 agaccatcca gtccttgccc aaggccctgc aggaacgcta cctgcttgtg cgctatgagg
901 acctggctcg agcccctgtg gcccagactt cccgaatgta tgaattcgtg ggattggaat
961 tcttgcccca tcttcagacc tgggtgcata acatcacccg aggcaagggc atgggtgacc
1021 acgctttcca cacaaatgcc agggatgccc ttaatgtctc ccaggcttgg cgctggtctt
1081 tgccctatga aaaggtttct cgacttcaga aagcctgtgg cgatgccatg aatttgctgg
1141 gctaccgcca cgtcagatct gaacaagaac agagaaacct gttgctggat cttctgtcta
1201 cctggactgt ccctgagcaa atccactaag agggttgaga aggctttgct gccacctggt
1261 gtcagcctca gtcactttct ctgaatgctt ctgagccttg cctacatctc tgagccttaa
1321 ctacatgtct gtgggtatca cactgagtgt gagttgtgtc cacacgtgct caagcagaag
1381 gacttttgtg tccatgcttg tgtctagaaa acagactggg gaaccttatg tgagcagcac
1441 atcccaccag tgaaacaggg tattgctctt cttcttttct tgatcttcct gtctgggcag
1501 acttcagaga ctttgtggcc tggaggccta ttaagcacga cacagtatca gtggaattga
1561 tccataaacc tccctgtcca catcttgccc aatggggaat ggatctttca ccaaagagct
1621 caccagcatt ttccacagag atgcaaattc tgagcccttg gagttcccag tggattcaag
1681 gaaggaagtg ggaacaaggt tggatgccta cttatgagct tgaccatcac agctatcggt
1741 aatcagaaat atgaaacaaa atctctgcac aaaagagcaa gctcttaagt tcacagggtg
1801 cctgggctgc atttgaatat cacttcccct ctgcattttc ccatcacata gaagactttg
1861 acctgtgaag ctgccatctg ttaatactaa aattcccaaa taaggttctg tttagaatgt
1921 ccctttttat gcttcttaat tattagcagt aaatgttcat ttttatggga tcctaagct
//