LOCUS AK074739 1951 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90258 fis, clone NT2RM4000587, highly similar
to Dermatan-4-sulfotransferase-1.
ACCESSION AK074739
VERSION AK074739.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1951)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..1951
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM4000587"
/clone_lib="NT2RM4"
/db_xref="H-InvDB:HIT000081774"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells"
/organism="Homo sapiens"
CDS 9..1139
/codon_start=1
/protein_id="BAC11172.1"
/translation="MFPRPLTPLAAPNGAEPLGRALRRAPLGRARAGLGGPPLLLPSM
LMFAVIVASSGLLLMIERGILAEMKPLPLHPPGREGTAWRGKAPKPGGLSLRAGDADL
QVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACS
NWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLLPEEIRYRLQHYFKFLFVRDPLER
LLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMN
EHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPE
SLHYHLCSAPRALLQDVLPKYILDFSLFAYPLPNVTKEACQQ"
BASE COUNT 357 a 589 c 569 g 436 t
ORIGIN
1 tgagcaccat gttcccccgc ccgctgaccc cgctggcggc cccaaatggc gccgagcccc
61 tgggccgggc gctgaggcgg gcccctctgg gcagggcccg ggcggggctg ggtgggccgc
121 ccctgctgct gccgtccatg ctgatgtttg cggtgatcgt ggcctccagc gggctgctgc
181 tcatgatcga gcggggcatc ctggccgaga tgaagcccct gcccctgcac ccgcccggcc
241 gcgagggcac agcctggcgc gggaaagccc ccaagcctgg gggcctgtcc ctcagggctg
301 gggacgcgga cttgcaagtg cggcaggacg tccggaacag gaccctgcgg gcggtgtgcg
361 gacagccagg catgccccgg gacccctggg acttgccggt ggggcagcgg cgcaccctgc
421 tgcgccacat cctcgtaagt gaccgttacc gcttcctcta ctgctacgtc cccaaggtgg
481 cctgctctaa ctggaagcgg gtgatgaagg tgctggcagg cgtcctggac agcgtggacg
541 tccgcctcaa gatggaccac cgcagtgacc tggtgttcct ggccgacctg ctgcctgagg
601 agattcgcta ccgcctgcag cactacttta agttcctgtt tgtgcgggat cccttggaac
661 gcctcctctc tgcctaccgc aacaagtttg gcgagatccg agagtaccag caacgctatg
721 gggctgagat agtgaggcgg tacagggctg gagcggggcc cagccctgca ggcgacgatg
781 tcacattccc cgagttcctg agatacctgg tggatgagga ccctgagcgc atgaatgagc
841 attggatgcc cgtgtaccac ctgtgccagc cttgtgccgt gcactatgac tttgtgggct
901 cctatgagag gctggaggct gatgcaaatc aggtgctgga gtgggtacgg gcaccacctc
961 acgtccgatt tccagctcgc caggcctggt accggccagc cagccccgaa agcctgcatt
1021 accacttgtg cagtgccccc cgggccctgc tgcaggatgt gctgcctaag tatatcctgg
1081 acttctccct ctttgcctac ccactgccta atgtcaccaa ggaggcgtgt cagcagtgac
1141 catgggtgtg gggccagcag ctggtgggga ctggtttcaa cgccagcttt ctgtgcttct
1201 gcctgtcatt cggagaaact ctggctctgg ggcttggggc ttctcaggat cctggatggc
1261 agagactgcc ctcagaagtt ccttgtccag ggtgggcacc cacagtgact cagaggacag
1321 ggctaggcag gagacctgct gctcctcatt ggggggatct cttggggggc agacaccagt
1381 ttgccaatga agcaacacat ctgatctaaa gactggctcc agaccccggg ctgccaggat
1441 tatgcagtcc acttggtcta ccttaattta acctgtggcc aaactcagag atggtaccag
1501 ccaggggcaa gcatgaccag agccagggac cctgtggctc tgatccccca tttatccacc
1561 ccatgtgcct caggactaga gtgagcaatc ataccttata aatgactttt gtgcctttct
1621 gctccagtct caaaatttcc tacacctgcc agttctttac atttttccaa ggaaaggaaa
1681 acggaagcag ggttcttgcc tggtagctcc aggacccagc tctgcaggca cccaaagacc
1741 ctctgtgccc agcctcttcc ttgagttctc ggaacctcct ccctaattct cccttccttc
1801 cccacaaggc ctttgaggtt gtgactgtgg ctggtatatc tggctgccat ttttctgatg
1861 catttattta aaatttgtac tttttgatag aacccttgta agggctttgt tttcctaata
1921 gctgactttt taataaagca gttttatata t
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