LOCUS AK074737 1909 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90256 fis, clone NT2RM4000417, highly similar
to Synaptotagmin-like protein 2.
ACCESSION AK074737
VERSION AK074737.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1909)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..1909
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM4000417"
/clone_lib="NT2RM4"
/db_xref="H-InvDB:HIT000081772"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from uninduced NT2 neuronal precursor cells"
/organism="Homo sapiens"
CDS 310..1440
/codon_start=1
/protein_id="BAC11170.1"
/translation="MSKSVPAFLQDESDDRETDTASESSYQLSRHKKSPSSLTNLSSS
SGMTSLSSVSGSVMSVYSGDFGNLEVKGNIQFAIEYVESLKELHVFVAQCKDLAAADV
KKQRSDPYVKAYLLPDKGKMGKKKTLVVKKTLNPVYNEILRYKIEKQILKTQKLNLSI
WHRDTFKRNSFLGEVELDLETWDWDNKQNKQLRWYPLKRKTAPVALEAENRGEMKLAL
QYVPEPVPGKKLPTTGEVHIWVKECLDLPLLRGSHLNSFVKCTILPDTSRKSRQKTRA
VGKTTNPIFNHTMVYDGFRPEDLMEACVELTVWDHYKLTNQFLGGLRIGFGTGKSYGT
EVDWMDSTSEEVALWEKMVNSPNTWIEATLPLRMLLIAKISK"
BASE COUNT 610 a 380 c 443 g 476 t
ORIGIN
1 gtgctttctc ctgctgctgg ccgattgctt gctctgaact aaccctctaa cccttgggag
61 tctgtgtgca gcagtgatgt gagctgcatc cgggctgaaa tgggagctcc cgctatggca
121 ctggctcaag gagctaatct aaagagaaaa tcaagcaaaa gtagataatc agccagaaga
181 attagtgcgt agtgctgaag atgatgagaa accagatcag aagccagtta caatgaatgc
241 gtaccaagaa tttccacagt gcctacacaa cctgataatc cattttctca ccctgacaaa
301 ctcaaaagga tgagcaagtc tgttccagca tttctccaag atgagagtga tgacagagaa
361 acagatacag catcagaaag cagttaccag ctcagcagac acaagaagag cccgagctct
421 ttaaccaatc ttagcagctc ctctggcatg acgtccttgt cttctgtgag tggcagtgtg
481 atgagtgttt atagtggaga ctttggcaat ctggaagtta aaggaaatat tcagtttgca
541 attgaatatg tggagtcact gaaggagttg catgtttttg tggcccagtg taaggactta
601 gcagcagcgg atgtaaaaaa acagcgttca gacccatatg taaaggccta tttgctacca
661 gacaaaggca aaatgggcaa gaagaaaaca ctcgtagtga agaaaacctt gaatcctgtg
721 tataacgaaa tactgcggta taaaattgaa aaacaaatct taaagacaca gaaattgaac
781 ctgtccattt ggcatcggga tacatttaag cgcaatagtt tcctagggga ggtggaactt
841 gatttggaaa catgggactg ggataacaaa cagaataaac aattgagatg gtaccctctg
901 aagcggaaga cagcaccagt tgcccttgaa gcagaaaaca gaggtgaaat gaaactagct
961 ctccagtatg tcccagagcc agtccctggt aaaaagcttc ctacaactgg agaagtgcac
1021 atctgggtga aggaatgcct tgatctacca ctgctaaggg gaagtcatct aaattctttt
1081 gttaaatgta ccatccttcc agatacaagt aggaaaagtc gccagaagac aagagctgta
1141 gggaaaacca ccaaccctat cttcaaccac actatggtgt atgatgggtt caggcctgaa
1201 gatctgatgg aagcctgtgt agagcttact gtctgggacc attacaaatt aaccaaccag
1261 tttttgggag gtcttcgtat tggctttgga acaggtaaaa gttatgggac tgaagtggac
1321 tggatggact ctacttcaga ggaagttgct ctctgggaga agatggtaaa ctcccccaat
1381 acttggattg aagcaacact gcctctcaga atgcttttga ttgccaagat ttccaaatga
1441 gcccaaattc cactggctcc tccactgaaa actactaaac cggtggaatc tgatcttgaa
1501 aatctgagta ggtggacaaa tatcctcact ttctatctat tgcacctaag gaatactaca
1561 cagcatgtaa aagtcaatct gcatgtgctt ctttgattac aaggcccaag ggatttaaat
1621 ataacaaaat gtgtaatttg tgactctaat attaaataag atatttgaac aagctaggaa
1681 aattgaattt ctgctgctgc ttcaaagaaa aagctgcccc agagcattaa acatggggta
1741 ttgttaagaa gcaaaatgtt cttgtttgcc atcatgtgtt tcacaccaca attctgtgcc
1801 acagttaaga gggtctggta cccttgcagg acctttgtag gttgtgggaa aaagtcgcag
1861 aaagatactc aaagtggagc agggaatgga gacagacatc agtgatgat
//