LOCUS AK074702 3071 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90221 fis, clone NT2RM1000462, highly similar
to Cat eye syndrome critical region protein 1precursor.
ACCESSION AK074702
VERSION AK074702.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3071)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..3071
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RM1000462"
/clone_lib="NT2RM1"
/db_xref="H-InvDB:HIT000081737"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pUC19FL3"
/note="mRNA from uninduced NT2 neuronal precursor cells"
/organism="Homo sapiens"
CDS 78..890
/codon_start=1
/protein_id="BAC11148.1"
/translation="MDSLEWNWALVYELSGEHHDEEWSVKTYQEVAQKFVETHPEFIG
IKIIYSDHRSKDVAVIAESIRMAMGLRIKFPTVVAGFDLVGHEDTGHSLRDYKEALMI
PAKDGVKLPYFFHAGGTDWQGTSIDRNILDALMLNTTRIGHGFALSKHPAVRTYSWKK
DIPIEVCPISNQVLKLVSDLRNHPVATLMATGHPMVISSDDPAMFGAKGLSYDFYEVF
MGIGGMKADLRTLKQLAMNSIKYSTLLESEKNTFMEIWKKRWDKFIADVATK"
BASE COUNT 644 a 862 c 671 g 894 t
ORIGIN
1 gtcctaatag ccacgcccac ctcaaatggc gatcccagag agcatctggc ttcaattttc
61 ctttttccgg aggggagatg gattccctgg aatggaactg ggctctggtg tatgagctca
121 gtggagagca ccatgacgaa gagtggtcag tgaagactta ccaggaagta gctcagaagt
181 ttgtggaaac tcaccctgag tttattggaa tcaaaatcat ttattcggat cacagatcca
241 aagatgtggc tgtcatcgca gaatccatcc gaatggccat ggggctccga atcaagttcc
301 ccacggtggt ggcagggttt gacctggtgg ggcatgagga cactggccac tccttgcgtg
361 actacaagga agctctgatg atccccgcca aggatggcgt taagctgcct tacttcttcc
421 acgccggagg aacagactgg cagggtactt ccatagacag gaacattctg gatgctctga
481 tgctgaacac taccagaatc ggccatggat ttgctttgag caaacacccc gcagtcagga
541 cttactcctg gaaaaaggac atccccatag aagtctgtcc catctctaac caggtgctga
601 aactggtgtc tgacttgagg aaccaccctg tagccactct gatggccact gggcacccca
661 tggtgatcag ctctgatgac ccagctatgt ttggtgccaa aggcttgtcc tatgatttct
721 atgaggtctt catgggcatt ggggggatga aggctgacct gaggaccctc aaacagctgg
781 ccatgaactc tatcaagtac agtaccctgt tggagagtga gaaaaatact ttcatggaaa
841 tctggaagaa gagatgggat aagttcatag cagatgtggc tacaaagtga ggagaagcta
901 gccagccctc tacaagctgt cctcttgcac acgctgtcac ttcctctcac tcgttcttga
961 atcagctcca tgtgcccatg aaatcaatgg cctctgtatg gagcgaccct gtgagaagca
1021 cttggctggc tgagcaaatt catcctctgg aaatattctc tctcagccac agtgacattg
1081 accctcttgg ttttctcctg tctctggcca tttcttccag tttccctatt tcagagtctt
1141 ctcctctctc tgatctctgt gctgtttcct caggactcag tcctgggctc tcttctattc
1201 tggtctcttt atttttttat ttttgtattt tttcgagatg gagttttgct cttgttgccc
1261 aggctggagt acaatggtgc gatctcagct cagtgcaacc tccgccaccc gggttcaggc
1321 aattctcttg catcagcctc gcgagtagtt ggaattatag gcatgtgcca ccacacccag
1381 ctgatttttg catttttagt agagacaggt tttcaccatg ttggcgaggc tggtatccaa
1441 ctcttgacct caggtgatcc actcgcccct tggctcccaa agtgctggaa ttacaggcat
1501 tagccaccat gcctggccta ttctggtctc tttaactctc tcctctttat ttctcttctc
1561 tctctgtaca cttttcctgg gtggtctcat ccattccttt gctttttcat accatttatt
1621 tgttaatgat tcccacattt atttatgcac ttggagagct cacaggaatc tcagaaactg
1681 atgaggtaca attctgaacc ctcagtctct tccctttaaa cctttctttt tctctacttt
1741 aatttttcta aagagtgtct tgctatgttg cccaggctgg tctccaactc aagtgatcct
1801 cctgccgcag tctcccgaag tgctgggatt actgacatga gccaccacac tcagcccttt
1861 aaacctttcc ctggcctttc ccatagctgg tgaaggacac ctccatccat tccacgcagt
1921 tgctcaaagc agaaattttc agtgcaagtc ttgatgctgc gccgtccccc actccctaca
1981 tcagaacgca tccctcatct ggactccagc ggtggcttct tgatgctgcg cggtccccca
2041 ctccctacat cagaatgcat cccgcatcca gactccagcg gtggtgctct acctgcacgc
2101 tgttgccaag tccaagctac catactcctg cctgagctat gacaacagcc tcctcactga
2161 tctccccttt cttccctttg cctcctccag ctcatttttc acagtgtaga atgacatttt
2221 gtttgtttgt tttgttttgt ttgagatgga gtctcgctct gttgcccagg gtggagtgca
2281 gcggtgcgat ctcggctcac tgcaacctcc acctcccggg ttcaagcgga ttctcgtgcc
2341 tcagcctcct gagtagctgg gattacaggc atgcaccacc atgcccggat aatttttgta
2401 tttttagtag agatggggtt tcactatgtt ggccaggctg gtctcgaaca cctgacctcg
2461 tggtccaccc gcctcggcct cccaaagcac tgggattaca ggcgtgagcc acccggcctg
2521 gcctagaatg acttttaaaa gatcaaatta aatcaggtca ctcctttgct tacaacgcag
2581 tgcgtttaga ggtacacccc atgtctccac agggcataca gcatccgatt taatctggat
2641 ccattccggc gccttcctct cccagtcacc cagagggccc caaccccggc ggccctttct
2701 tcctcaaatg tcctcggctc tataccgtgc ctgggtcttt tctctttctc tctgcctgga
2761 agattccttc tttccccttt tgtcttgccc actcctgttt acccttcaag tttcaagttc
2821 atgtcactgt ctcagagagg ttttcctgtg ctcgccctgt ttctctcagg aagccttgct
2881 cttttccatc atgcctctaa tcacagctta taatcggata tttatttctg tgtctacagt
2941 cttgccctgc cagactgtat gccccatgtg ggcaggcgct catgattgtt tctgattgtt
3001 tcacgcatgc tgctaaccca gagcctgggc ccaaagctag ttagtactca ataaacaatg
3061 cattgaatga g
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