LOCUS AK074494 3382 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ90013 fis, clone HEMBA1000477.
ACCESSION AK074494
VERSION AK074494.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3382)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (25-MAR-2002) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Isogai,T., Ota,T., Nishikawa,T., Hayashi,K., Otsuki,T.,
Sugiyama,T., Suzuki,Y., Nagai,K., Sugano,S., Ishii,S.,
Kawai-Hio,Y., Saito,K., Yamamoto,J., Wakamatsu,A., Nakamura,Y.,
Kojima,S., Nagahari,K., Masuho,Y., Ono,T., Okano,K., Yoshikawa,Y.,
Aotsuka,S., Sasaki,N., Hattori,A., Okumura,K., Iwayanagi,T. and
Ninomiya,K.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2002)
REFERENCE 3
AUTHORS Otsuki,T., Ota,T., Nishikawa,T., Hayashi,K., Suzuki,Y.,
Yamamoto,J., Wakamatsu,A., Kimura,K., Sakamoto,K., Hatano,N.,
Kawai,Y., Ishii,S., Saito,K., Kojima,S., Sugiyama,T., Ono,T.,
Okano,K., Yoshikawa,Y., Aotsuka,S., Sasaki,N., Hattori,A.,
Okumura,K., Nagai,K., Sugano,S. and Isogai,T.
TITLE Signal Sequence and Keyword Trap in silico for Selection of
Full-Length Human cDNAs Encoding Secretion or Membrane Proteins
from Oligo-Capped cDNA Libraries
JOURNAL DNA Res. 12, 117-126 (2005)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology; cDNA library construction:
Institute of Medical Science, University of Tokyo, Laboratory of
Genome Structure, Human Genome Center; cDNA 5'- & 3'-end one pass
sequencing and clone selection: Helix Research Institute
(supported by Japan Key Technology Center etc.).
FEATURES Location/Qualifiers
source 1..3382
/clone="HEMBA1000477"
/clone_lib="HEMBA1"
/db_xref="H-InvDB:HIT000081529"
/db_xref="taxon:9606"
/dev_stage="embryo, 10 weeks"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="whole embryo, mainly head"
CDS 15..1703
/codon_start=1
/protein_id="BAC11022.1"
/translation="MAGVGDAAAPGEGGGGGVDGPQRDGRGEADQPGGSGGQGPPPAP
QLTETLGFYESDRRRERRRGRTELSLLRFLSAELTRGYFLEHNEAKYTERRERVYTCL
RIPRELEKLMVFGIFLCLDAFLYVFTLLPLRVFLALFRLLTLPCYGLRDRRLLQPAQV
CDILKGVILVICYFMMHYVDYSMMYHLIRGQSVIKLYIIYNMLEVADRLFSSFGQDIL
DALYWTATEPKERKRAHIGVIPHFFMAVLYVFLHAILIMVQATTLNVAFNSHNKSLLT
IMMSNNFVEIKGSVFKKFEKNNLFQMSNSDIKERFTNYVLLLIVCLRNMEQFSWNPDH
LWVLFPDVCMVIASEIAVDIVKHAFITKFNDITADVYSEYRASLAFDLVSSRQKNAYT
DYSDSVARRMGFIPLPLAVLLIRVVTSSIKVQGILSYACVILFYFGLISLKVLNSIVL
LGKSCQYVKEAKMEEKLSNPPATCTPGKPSSKSQNKCKPSQGLSTEENLSASITKQPI
HQKENIIPLLVTSNSDQFLTTPDGDEKDITQDNSELKHRSSKSVLLCQSGLPEC"
BASE COUNT 959 a 623 c 748 g 1052 t
ORIGIN
1 gttgtgctcg gaacatggcg ggcgtcggcg acgcggccgc tccgggagaa ggcggcggtg
61 gcggcgtgga cggcccgcag cgggacggcc gcggcgaggc ggatcagccg ggcggcagcg
121 gcggacaggg gcccccgccg gcgcctcagc tcacagagac gctgggcttc tacgagagcg
181 accggcggcg ggagaggcgc cggggccgca cagagctttc attgttgagg ttcctcagtg
241 ctgaactaac aagagggtac ttccttgaac ataatgaggc caagtataca gaaagaagag
301 aaagagtata cacttgtttg cgaataccaa gagaattgga aaagctgatg gtttttggaa
361 tctttctgtg cctggatgcg tttttgtatg tgttcaccct gcttccttta agagttttcc
421 tggcactatt caggctcctc actttgcctt gctatggctt aagggacaga cgtttgcttc
481 agcctgccca ggtgtgtgac attttgaagg gtgtcatttt ggtaatctgc tattttatga
541 tgcactatgt tgactactcc atgatgtacc acctgataag ggggcagtcc gtcatcaagc
601 tctacatcat ctacaacatg ctggaggtag ctgatcgtct gttttcatct tttggacaag
661 acatattaga tgctctctat tggacagcaa cagagcctaa agaaagaaaa agagcccaca
721 ttggggtgat tcctcacttt ttcatggctg ttctctatgt ctttttgcat gcaattctta
781 taatggttca agcaacaact ctcaatgtag cttttaactc acacaacaag tctttgctta
841 ctatcatgat gtctaataat tttgttgaaa ttaaaggaag tgttttcaag aagtttgaaa
901 agaacaatct ctttcaaatg tcaaatagcg atattaagga acgattcaca aattatgtgc
961 ttttactgat agtgtgtcta agaaacatgg aacagttttc ttggaatcca gatcatctct
1021 gggtgttgtt tccagatgtc tgtatggtaa ttgcatcaga aattgccgtg gatattgtaa
1081 aacatgcctt tattactaaa ttcaatgaca ttactgcaga tgtctacagt gaatatagag
1141 ccagtcttgc ttttgacctt gttagcagcc gacagaaaaa tgcatacact gattacagtg
1201 actctgtagc acggaggatg ggctttattc ctctcccact agctgtttta ctcatcagag
1261 ttgtaacaag ctcaattaaa gtgcaaggaa tcctgtctta tgcctgtgtc atactcttct
1321 attttgggtt gatatccctg aaagtactta atagcatcgt gctgttgggg aaatcgtgcc
1381 agtatgtgaa ggaagccaaa atggaagaga agctgtcgaa tcctcccgca acctgcactc
1441 caggcaagcc gtccagtaaa tcacagaaca aatgtaaacc ctctcaaggc ctttccacag
1501 aagaaaacct gtctgcctcc atcaccaaac aacctattca tcaaaaggaa aatatcatac
1561 cattacttgt gacaagcaat tctgatcagt ttttgacaac tccagatggt gacgagaagg
1621 acataacgca ggacaattct gaattaaaac acagatcctc aaaatctgtt ttgctttgtc
1681 agtctgggct tccagaatgt tgaatttgcc tcaaggcctc ttcagtataa ggaaatacct
1741 ggaaaactgt gaaactttta ccacgacgta atctttccag tctcatacta ttttcacaaa
1801 cagttttcaa acgttacttc atctgccaaa gcattaaaaa aattaaacat aagtcaagat
1861 aaatgttctt accaccagaa taacccttaa agatgtatct taattaatca gaataaaagg
1921 ctaccttaaa taagacatga tgaatagtag cattttgtag ggttaaaaaa aaatgctaaa
1981 ctagtttatt tattaaaaaa ataattaatg aaggctatgc attactggga aaaatccttc
2041 ataatttttc agtttactct ttaaagcaaa atgtgagctg tatgccattt aattggtgga
2101 taaaacaaat ttccttacaa aagggcactt tttacaccaa ggaagcagag cagtgttaac
2161 tttaaggtat acttaactga ttttgcttta aaactaatta cttcataaat tattacagct
2221 aaaaggattc gatgttgaac aggctgaaaa attgtcaatg tacaaaattc aaaactgtca
2281 actttaactg taaagtaaat gctccaagtg tgttagacag catcatcttg cttgggctta
2341 ccaaatgcat tagtctttgt gtttgggtcg acagcgagtg tgcctgtgct ggggcagggt
2401 gtgtgttctg tgggagggtg tctgtgggga tgtgactatc agggtgggcc tgtgctgggg
2461 atggggcagg cctgggtctg gagaggattt tgtgtgaaag taaatggggt gtttgaggcg
2521 tatgggtggc tgttggtgtg gggaggcatc tgtgtatggc tgttgggaac agcaaccaaa
2581 aggtgctttt tggttttatt tgagatgaag attgtgtttc cgcttaatta ctagtttgtg
2641 gtctatatca tagaagttat ttcccacccc attttatctt gacaaccgtg tttgcatttc
2701 tgtaaaactt ctacaacttc tggtgtcaga actgtccaga agatggtact gttaactgtt
2761 atttcctttg atgttttgat tttgaagttt agctctcatg caaatgtttc aggcgtacat
2821 acataggcag aaagcaattt ttaggtgatt tgtctgtttc ttggatgaaa tataaagcaa
2881 gctttaatgt tctgacttgt tcatttgaaa tacaaaaaag taagtgaatt ttaatgtttt
2941 gcattaacta aagaaatctg aagattaatg ttgaggaaat tgtatggaca tgcctttgtg
3001 aaaccaggaa gtattttaag ttaaaaatga aaaaggtttt aattgctttg tgtgtgtttt
3061 aatggagccc cattttagaa ttattttttc catctccctt taccaacaaa aggaacaatg
3121 ccaccgtgaa tggaatagtt tggggaaagc tttgccaaac acaaccctga tttcagagca
3181 ggaacatggt ttggatagat cttcagttcc gcttgaatta ttctgttact gcgctgtcat
3241 tttcagaaga tttccattcc tttgcagatg ctgttgggag ttcgggatta tgttcatttc
3301 ttctgtttta atggcattca gtactaattt tataagtgca tcttgtgtga atctcaataa
3361 attcagtttt gtaatctttt tt
//