LOCUS AK057048 2718 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ32486 fis, clone SKNMC2001623.
ACCESSION AK057048
VERSION AK057048.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2718)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Kawakami,B., Sugiyama,A., Takemoto,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Ota,T., Wakamatsu,A., Ishii,S., Yamamoto,J.,
Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K.,
Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H.,
Kanda,K., Wagatsuma,M., Murakawa,K., Kanehori,K.,
Takahashi-Fujii,A., Oshima,A., Suzuki,Y., Sugano,S., Nagahari,K.,
Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2718
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2001623"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000013662"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 238..2568
/codon_start=1
/protein_id="BAG51853.1"
/translation="MGGFCEKLLLSDRWGWSDVSGLQHRPLDRVALPSPHWEWESDWY
VDENFGGEPTEKGGWTYAIDFPATYTKYKKWNSCVRRRKWIRYRRYKSRDIWAKIPSK
DDPKELPDPFNDLSVGGWEITEEPVGRLSVWAVSLQGKVLCPCLASQVWYREDVSHSN
PEGSSWSLLDTPGEVVQISCGPHDLLWATLWEGQALVREGINRSNPKGSSWSIVEPPG
SENGVMHISVGVSVVWAVTKDWKVWFRRGVNSHNPCGTSWIEMVGEMTMVNVGMNDQV
WGIGCEDRAVYFRQGVTPSELSGKTWKAIIAARECDRSHSGSSSSLLSAGCFFGDEVR
GSGESAPSDTDASSEVERPGPGQILPAEPLDDSKNATGNSASGLGAGRTAEDTVEDAC
PAEGSREARPNTHPGPAPTPAELPWTNIDLKEAKKVPSHSAAGFPETTSLSSLGLLPL
GLEEPYGVDDHPLWAWVSGGGCVVEACAMPRWFTVQAGLSSSVHMLSLSITPAQTAAW
RKQIFQQLTERTKRELENFRHYEQAVEQSVWVKTGALQWWCDWKPHKWVDVRLALEQF
TGHDGVRDSILFIYYVVHEEKKYIHIFLNEVVALVPVLNETKHSFALYTPERTRQRWP
VRLAAATEQDMNDWLALLSLSCCESRKVQGRPSPQAIWSITCKGDIFVSEPSPDLEAH
EHPLPCDQMFWRQMGGHLRMVEANSRGVVWGIGYDHTAWVYTGGYGGGCFQGLASSTS
NIYTQSDVKCVHIYENQRWNPVTGYTSSRDRISPCW"
BASE COUNT 549 a 811 c 875 g 483 t
ORIGIN
1 ggaccctctt gcccggtccc cgggagggcc ggtccgctcc tcccggacgc cgaggaccta
61 ccaccgcgac ttcgccccgc ccggcgcggg cccaggaccc tgatgtcgct tttgaacagc
121 ccctgcacct ggcagccagc gagctactgt agtaggcatt gccgactgtt tgcataccgg
181 atgggagtga cagtgtaata gaaaaacaag caagaaacct tttagcgctg gaatcccatg
241 ggcggcttct gtgagaagct cctgctgagt gaccgctggg ggtggagtga cgtgagtggg
301 ctccagcacc ggccgctgga cagggtggca ctgccctcgc cgcactggga gtgggagtct
361 gactggtacg tggatgagaa ttttggaggt gaacccactg agaaaggggg gtggacgtat
421 gccatcgact ttcccgccac ctacacgaaa tacaagaagt ggaattcttg tgtgcggcgc
481 cggaagtgga tccggtacag gagatacaag tcccgggaca tctgggccaa gatcccctcg
541 aaggatgacc ccaaggagct gcccgacccc ttcaacgacc tctctgtagg gggctgggag
601 atcacggagg agcctgtggg ccgcctgtca gtgtgggctg tgtctctgca gggcaaggtg
661 ctgtgtccct gtcttgcctc ccaggtgtgg tacagagagg acgtcagcca ctccaacccc
721 gaagggtcct cctggtccct gctggacacc cccggggagg tggttcagat cagctgtggg
781 ccccacgacc tcctgtgggc cacactctgg gagggacagg ccctggtccg ggaaggaatc
841 aacaggagca atcccaaagg aagttcctgg tccatcgtgg agcctcctgg atctgaaaac
901 ggggtcatgc acatctcggt gggagtcagc gtggtctggg ctgtcaccaa ggactggaag
961 gtgtggttcc gaagaggcgt caactctcac aatccctgcg gcaccagttg gattgagatg
1021 gttggtgaga tgacgatggt gaacgtggga atgaacgacc aggtgtgggg cattggctgt
1081 gaggaccgag ccgtgtactt ccggcagggt gtcaccccca gcgagctcag tgggaagacc
1141 tggaaagcca tcatcgcggc ccgagagtgt gaccggtcac actctggcag ctcgtctagt
1201 ctcctcagtg ccggctgctt cttcggtgat gaggtgaggg gtagtggcga gtctgccccc
1261 agcgacaccg atgcctcctc ggaagttgag agaccagggc ctggccagat tctccctgca
1321 gaacctctag acgattccaa gaatgccaca gggaactcag cctcaggcct gggggctggc
1381 aggaccgcag aagataccgt ggaagatgcc tgcccagccg agggcagcag ggaggccaga
1441 cccaacacgc accccggccc ggcccccacc ccggccgagc tgccctggac caatattgac
1501 ctcaaggagg ccaagaaagt gcccagccac tcggccgctg gcttccccga gaccaccagc
1561 ctctcctctc tggggctcct cccactgggc ttggaggagc cgtatggggt ggatgaccac
1621 ccgctgtggg cctgggtgtc gggaggcggc tgcgtggtgg aggcatgtgc catgcccaga
1681 tggttcactg tccaggcggg cctgtcctcc tcggtacaca tgctgtccct gtccatcacg
1741 ccggcccaga ccgctgcctg gaggaagcag atcttccagc agctcacgga aaggaccaag
1801 cgggagctgg agaacttcag acactacgag caggccgtgg agcagtcggt gtgggtgaag
1861 accggggcgc tgcagtggtg gtgcgactgg aagccccaca agtgggtgga cgtgcgcttg
1921 gccctggagc agttcacggg gcacgacggc gtccgggaca gcatcctctt catctactat
1981 gtggtccacg aggagaagaa gtacatccac atattcctga atgaggtggt ggcgctggtc
2041 ccagtgctga acgagaccaa gcactccttt gccctgtaca cccctgagcg gacacggcag
2101 aggtggccgg tgcgtctggc tgctgccacc gagcaggaca tgaatgactg gctcgccctg
2161 ctcagcctgt cttgctgcga gagccggaag gtgcagggcc gcccgtcccc gcaggccatc
2221 tggtccatca cctgcaaggg ggacatcttc gtgagcgagc ccagcccaga cctggaggcc
2281 cacgagcacc ccctgccctg cgaccagatg ttttggcggc agatgggagg ccacctgcgg
2341 atggtggagg ccaacagccg gggcgtggtg tggggcatcg gctatgacca cacggcctgg
2401 gtatacacag gcggctatgg aggcggctgc ttccaaggcc tggccagcag caccagtaac
2461 atctacacgc agtcagacgt gaagtgtgtt cacatctatg agaaccagcg ctggaacccc
2521 gtcacaggct acaccagcag tagagacagg atttcaccat gttggtgagg ctggtctcca
2581 actcctgaac tcccgaagcc cgcctcagcc tcccaaagtg ttgagattac aggcatcatc
2641 aggcgagagc cactgcgccc ggcctccact tctgcctttt ccaaattgac gaaataaaca
2701 cttagtaata gaaacaac
//