LOCUS AK057032 1168 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ32470 fis, clone SKNMC2000305.
ACCESSION AK057032
VERSION AK057032.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1168)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Kawakami,B., Sugiyama,A., Takemoto,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Ota,T., Wakamatsu,A., Ishii,S., Yamamoto,J.,
Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K.,
Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H.,
Kanda,K., Wagatsuma,M., Murakawa,K., Kanehori,K.,
Takahashi-Fujii,A., Oshima,A., Suzuki,Y., Sugano,S., Nagahari,K.,
Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1168
/cell_line="SK-N-MC"
/cell_type="neuroepithelioma"
/clone="SKNMC2000305"
/clone_lib="SKNMC2"
/db_xref="H-InvDB:HIT000013646"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
CDS 154..>1168
/codon_start=1
/protein_id="BAB71350.1"
/translation="MPPPSDIVKVAIEWPGANAQLLEIDQKRPLASIIKEVCDGWSLP
NPEYYTLRYADGPQLYITEQTRSDIKNGTILQLAISPSRAARQLMERTQSSNMETRLD
AMKELAKLSADVTFATEFINMDGIIVLTRLVESGTKLLSHYSEMLAFTLTAFLELMDH
GIVSWDMVSITFIKQIAGYVSQPMVDVSILQRSLAILESMVLNSQSLCQKIAEEITVG
QLISHLQVSNQEIQTYAIALINALFLKAPEDKRQDMANAFAQKHLRSIILNHVIRGNR
PIKTEMAHQLYVLQVLTFNLLEERMMTKMDPNDQAQRDIIFELRRIAFDAESDPSNAP
GSGT"
BASE COUNT 301 a 305 c 306 g 256 t
ORIGIN
1 ggaggagccg ccgagcggag acccgggagc aggagctggg cctaggtctg cgccctggag
61 ggaggtgtag aaagaggtac atggagaaca agtttgtcaa tccgtctgaa cttcagttgc
121 cttacctgta agaattagag cccattggga acgatgccac caccgtcaga cattgtcaaa
181 gtggccattg agtggccagg tgctaacgcc cagctccttg aaatcgacca gaaacggccc
241 ctggcatcca ttatcaagga agtttgtgat gggtggtcgt tgccaaaccc agagtattat
301 accctccgtt atgcagatgg tcctcagctg tacatcaccg aacagactcg cagtgacatt
361 aagaatggga caatcttaca actggctatc tccccgtccc gggctgcacg ccagctgatg
421 gagaggaccc agtcatccaa catggagacc cggctggatg ccatgaagga gctggccaag
481 ctctctgccg acgtgacttt cgctactgag ttcatcaaca tggatggcat cattgtgctg
541 acaaggctcg tggaaagtgg aaccaagctc ttgtcccact acagtgagat gctggcattc
601 accctgactg ccttcctaga gctcatggac catggcattg tctcctggga catggtttca
661 atcaccttta ttaagcagat tgcagggtat gtgagccagc ccatggtgga cgtgtcaatc
721 cttcagaggt ccctggccat cctggagagc atggtcttga acagccagag tctgtgccag
781 aagatagccg aggaaatcac cgtgggacag ctcatctcac acctccaggt ctccaaccag
841 gagattcaga cctacgccat tgcactgatt aatgcacttt ttctgaaggc tcctgaggac
901 aaacgacagg atatggcaaa tgcatttgca cagaagcatc tccggtctat aatcctgaat
961 catgtgatcc gagggaaccg ccccatcaaa actgagatgg cccatcagct atatgtcctt
1021 caagtcctaa cctttaacct tctggaagaa aggatgatga ccaagatgga ccccaatgac
1081 caggctcaaa gggacatcat atttgaactg aggaggattg catttgacgc agagtctgat
1141 cctagcaatg cccctgggag tgggaccg
//