LOCUS AK056562 3014 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ32000 fis, clone NT2RP7009370, weakly similar
to PUTATIVE SERINE/THREONINE-PROTEIN KINASE PKWA (EC 2.7.1.-).
ACCESSION AK056562
VERSION AK056562.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3014)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..3014
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7009370"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000013176"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 124..2220
/codon_start=1
/protein_id="BAB71217.1"
/translation="MSAVVAQTLHVFGLRSHVANNIFYFDEQIIIFPSGNHCVKYNVD
QKWQKFIPGSEKSQGMLALSISPNRRYLAISETVQEKPAITIYELSSIPCRKRKVLNN
FDFQVQKFISMAFSPDSKYLLAQTSPPESNLVYWLWEKQKVMAIVRIDTQNNPVYQVS
FSPQDNTQVCVTGNGMFKLLRFAEGTLKQTSFQRGEPQNYLAHTWVADDKIVVGTDTG
KLFLFESGDQRWETSIMVKEPTNGSKSLDVIQESESLIEFPPVSSPLPSYEQMVAASS
HSQMSMPQVFAIAAYSKGFACSAGPGRVLLFEKMEEKDFYRESREIRIPVDPQSNDPS
QSDKQDVLCLCFSPSEETLVASTSKNQLYSITMSLTEISKGEPAHFEYLMYPLHSAPI
TGLATCIRKPLIATCSLDRSIRLWNYETNTLELFKEYQEEAYSISLHPSGHFIVVGFA
DKLRLMNLLIDDIRSFKEYSVRGCGECSFSNGGHLFAAVNGNVIHVYTTTSLENISSL
KGHTGKIRSIVWNADDSKLISGGTDGAVYEWNLSTGKRETECVLKSCSYNCVTVSPDA
KIIFAVGSDHTLKEIADSLILREISAFDVTYTAIVISHSGRMMFVGTSVGTIRAMKYP
LPLQKEFNEYQAHAGPITKVSRTLSPGTQSHTCLLRALFIPSTSQCLFSLLLLSYLFI
HHSLNHHLLTMNILFV"
BASE COUNT 853 a 711 c 669 g 781 t
ORIGIN
1 caaaccgcta cggcgtttga aagtgtccgg gttgcttagg atccctacag gtagcgcctc
61 tggatacatg cgtggtctgc tgacccagag agaaacgaaa gcagaactgt ttggcgggag
121 atcatgtcag ccgtggtagc tcagacgctg catgtttttg gtcttcgatc ccacgtggcc
181 aacaatatct tctacttcga tgaacagatc attatatttc cttcaggaaa tcactgtgtg
241 aagtacaatg tggatcagaa atggcaaaaa ttcattccag gctcagagaa gagtcagggc
301 atgttggcct tgtccatcag tcccaatcgg cggtacctcg ctatctctga gactgtgcaa
361 gaaaaacctg ccatcaccat ttatgaattg tcatccatcc cttgccggaa gcgcaaagtt
421 cttaataatt ttgacttcca agttcagaaa tttattagca tggctttttc tccagactcc
481 aaatacctat tggctcagac gtcacctcca gagtcaaatc ttgtctactg gctgtgggaa
541 aaacagaaag taatggccat tgttagaatc gacactcaga acaaccctgt ctaccaggtg
601 agcttcagtc cacaggataa cactcaggtg tgtgtcactg gaaatgggat gtttaagctt
661 ctccgttttg ctgagggaac cctgaagcaa accagctttc agaggggaga accccaaaac
721 tatctagctc acacctgggt ggctgatgac aagattgtcg ttggcactga cacaggcaaa
781 ctcttcctct ttgaatctgg agatcagcgt tgggagacca gcataatggt caaggaacct
841 accaatggct caaagagcct ggatgtcatt caggaatcag agagcctgat tgaatttcca
901 ccagtcagtt ctccactccc ttcctatgaa cagatggtgg cggccagtag ccatagccag
961 atgtccatgc cccaggtgtt tgccattgca gcctattcaa agggatttgc ctgttctgct
1021 gggccaggga gagttctgct gtttgagaag atggaagaaa aggattttta ccgtgagagc
1081 agagaaatca ggattcctgt ggacccgcag agcaatgatc caagtcagtc tgacaaacag
1141 gacgttctct gcctgtgctt cagcccctca gaggaaactc tggttgccag caccagtaag
1201 aaccaactct acagcatcac catgtccctg acagagatca gcaaggggga gcctgctcac
1261 tttgagtatt tgatgtatcc attgcactca gcacccatca ccggtctagc tacctgcatc
1321 cgcaaacccc ttatagccac ctgttctctg gatcgatcca tccgcctttg gaattatgaa
1381 acaaacaccc tggaactatt taaggaatac caagaagagg catattccat cagccttcat
1441 ccatctggac acttcattgt agtagggttt gctgacaaac tacgcctcat gaatctactc
1501 attgatgata tacgttcttt caaagaatac tctgttagag gatgcggaga gtgttccttt
1561 agcaatggag gtcacctgtt tgctgcagtc aatggaaatg tgattcacgt ttacaccacc
1621 acgagcctag agaacatctc aagcctgaaa ggacacacag ggaagattcg ctcaattgtg
1681 tggaatgcag atgatagcaa actgatttct ggtggcacag atggtgctgt gtatgaatgg
1741 aatctgtcca caggaaagag agagacagaa tgcgtgctca agtcttgcag ctacaactgt
1801 gttactgtct cccccgatgc caaaattatc tttgctgttg gatcagacca caccctcaag
1861 gagattgcag attccttgat ccttcgagag atatcggcgt ttgatgtcac ctacaccgcc
1921 attgtcatct cacattctgg acgcatgatg tttgtgggca cctcggtggg aaccattcgt
1981 gccatgaagt accctctgcc tctgcagaag gaattcaatg agtaccaggc ccatgccggt
2041 cctatcacca aggtgagcag gaccctctcc ccaggaaccc agtcccacac ctgcctgcta
2101 cgtgccttgt tcatcccttc aacctcccaa tgtcttttct ctctccttct tctctcttat
2161 ttattcatcc atcattcatt gaatcaccat ctattgacta tgaatatact ctttgtttaa
2221 actacttcca ggaatttagc ctaggaaatc atcagagata cacctaaaaa tgtatgtaca
2281 acgttttcac cataatatta tgcataataa ggggccgttt ggtggatgcc gtagctgccg
2341 tgagtgtggg ctgcacttga ccacagctgc ctcctcctcc agagaatgcc ccagactgaa
2401 aggagccata gccctgaaga ttggccccta cctctccctg agggtacaaa aggccacccc
2461 aggggcaata ccatgagtac acatttgtaa attgtccttc cattcaccct tctcataaag
2521 tagtatctat gttcaacagt caaaatgtgg aagcaaccaa gcatccatcg acagacgaat
2581 gcataagcaa aagatggtat atctatacaa tggaacaata ccctgcctaa aaaggaaggg
2641 aattctgcaa tgtgctacca catggatgaa ccttgaggat gttatgctaa attaaataag
2701 gccaaccaca aaaagataag tacagtgtga ttccactttt aggagatact tagagcagtc
2761 agaatcacaa agacagagtg gtggttggca ggggctgcag gaagggggaa tgaggaatga
2821 ttgtttcata ggtatagagt tttggtttta caagacaaaa ggattatggg ggtagttggt
2881 ggcaatggct gcacaacatt acaaatgtat ttaataacat gaactgtaca cttgaaaatg
2941 gttaagatag caaattttac agaatatgta ttttacgaca attttaaaaa tgaaataaaa
3001 aagaattatc ttgc
//