LOCUS AK056549 2724 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31987 fis, clone NT2RP7008855, highly similar
to Connector enhancer of kinase suppressor of ras 2.
ACCESSION AK056549
VERSION AK056549.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2724)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2724
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7008855"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000013163"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 57..1529
/codon_start=1
/note="highly similar to Connector enhancer of kinase
suppressor of ras 2"
/protein_id="BAG51744.1"
/translation="MVGKPVHKGSESPNSFLDQEYRKRFNIVEEDTVLYCYEYEKGRS
SSQGRRESTPTYENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYS
LLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGKSKRRISCKDL
GRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAEGFISLPEFKID
RASECRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAERERIKQEQDYW
SESDKEEADTPSTPKQDSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQSSH
EEFRQEVTGSSAVSPIRKTASQRRSWQDLIETPLTSSGLHYLQTLPLEDSVFSDSAAI
SPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGGKPRSFTLPRDSGFNHCCL
NAPVSACDPQDDVQPPEVEEEEEEEEEEGEAAGENIGEKS"
BASE COUNT 872 a 523 c 611 g 718 t
ORIGIN
1 catatattcc cagggatgaa aaaggaaacc ttccttgtga agacctcaga ggacatatgg
61 tgggcaagcc agtgcataag ggatctgaat caccaaattc atttctggat caggaatatc
121 gaaagagatt taatattgtc gaagaagata ctgtcttata ttgctatgaa tatgaaaaag
181 gaagatcaag tagtcaagga agacgagaaa gcaccccaac ttatgaaaac tctctacttc
241 ggtatatgag caatgaaaag attgctcaag aagaatacat gtttcagaga aacagcaaaa
301 aggacacagg gaagaagtca aaaaagaagg gtgataagag taatagccca actcactatt
361 cattgctacc tagtttacaa atggatgcac tgagacaaga catcatgggc actcctgtgc
421 cagagaccac actataccat acatttcagc agtcctcact gcagcacaaa tcaaagaaga
481 aaaacaaagg tcctatagca ggcaagagca aaagacgaat ttcttgcaaa gatcttggcc
541 gtggtgactg tgagggctgg ctttggaaaa agaaagatgc gaagagttac ttttcacaga
601 aatggaaaaa atattggttt gtcctaaagg atgcatccct ttattggtat attaatgagg
661 aggatgaaaa agcagaagga ttcattagcc tgcctgaatt taaaattgat agagccagtg
721 aatgccgcaa aaaatatgca ttcaaagcct gtcatcctaa aatcaaaagc ttttattttg
781 ctgctgaaca tcttgatgat atgaacaggt ggcttaacag aattaatatg ctgactgcag
841 gatatgcaga aagagagagg attaagcagg aacaagatta ctggagtgag agtgacaagg
901 aagaagcaga tactccatca acaccaaaac aagatagccc tccaccccca tatgatacat
961 acccacgacc tccctcgatg agttgcgcca gtccttatgt ggaagcaaaa catagccgac
1021 tttcctccac ggagacttct cagtctcagt cttctcatga ggagtttcgc caggaagtaa
1081 ctgggagcag tgcagtgtct cccattcgca agacagccag tcagcgccgc tcctggcagg
1141 atttaattga gacgccactg acaagttcag gcttacacta tcttcagact ctgcccctgg
1201 aggattctgt cttctctgac tccgcggcca tctccccaga gcacaggcgg cagtctaccc
1261 tgccaactca gaaatgccac ctgcaggatc actatgggcc atacccctta gctgagagtg
1321 agaggatgca agtgctaaat ggaaatgggg gcaagcctcg aagttttact ctgcctcgag
1381 atagcgggtt caaccattgc tgtctgaatg ctccagttag tgcctgtgac ccacaggatg
1441 acgtgcaacc cccagaggtg gaggaagagg aggaggagga ggaggaggaa ggggaggcag
1501 caggggaaaa cataggagaa aaaagctaat acactgcgag agttggtaga acctctccat
1561 gccaaatcgg atccacttct gttggcactc aacccattgg actcacagat tgataagcta
1621 atgtttagag aatttagatc ggagagagtc ggtacggcgc agactcaaca tcaacctctt
1681 gcaagcaact aaaatggcct cgtccttgct gtttataaca gaaaacagac ttgtaaaaag
1741 cttagatcat caagtgtttt ggattggggg cctcccaaag ggatataaga ggggcaggcc
1801 actcttaaga agaatgcgag ctttctacat tgggactagc ataagatcaa agccaatcaa
1861 gatggagcac agtaacagaa aactgcggtt tctgtgggag aacagaaggg gaaagggtct
1921 taactgggaa agggctctgt gtggtaacac ctcagttgcg ttctcctgac accaggaaaa
1981 gagagggatc agcttcaata actagaaaat tctggctgtt taatggactc tttggtggcc
2041 tctttaaggc aaagcagaga aagcaaatta tgtattaagt gtattttgca tttttaaaac
2101 ttgacgtgct gtattgtact aaattaagtg taatctatta aggcaaggta tacacaattt
2161 gctttgaaac ttactatgtt tattctatta taaagtgtat tcaggtgcaa cacagagact
2221 gctttcggtg acattaatga agaaaatttc tcatgccagg ctttattata gaatcttcag
2281 ctaaaatcct aactttctcc ttatttcttg gcacttgtat acaagtggtg ttgcctctta
2341 gggcaggcat gagctattct tttctgtaaa atattttgaa tctataggct gtgggtttca
2401 tttttgaaaa gtattttgtc tggatgtctt tcaaactagc ttcagatatt atttaatact
2461 atgtaactgg gtcccctatg gctcaatcaa tattgcttat ttttcttctg tagtggatgt
2521 gaaatttcct ttagttggat aagatacact gtaataattt taatgctaat taatgatatt
2581 tcatactgtg caatgaacag ataatttaac actgtatttt gaaatgtttt tttcttcctg
2641 tcaccgcggt gtgtggtatt gcataatgtg aatacctgta aaaatataaa ttacttaaaa
2701 ataaaaatat gaccaattgg tatc
//