LOCUS AK056450 2669 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31888 fis, clone NT2RP7003055.
ACCESSION AK056450
VERSION AK056450.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2669)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Tashiro,H., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Sugiyama,T., Irie,R.,
Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y.,
Kawai-Hio,Y., Saito,K., Nishikawa,T., Kimura,K., Yamashita,H.,
Matsuo,K., Nakamura,Y., Sekine,M., Kikuchi,H., Kanda,K.,
Wagatsuma,M., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2669
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP7003055"
/clone_lib="NT2RP7"
/db_xref="H-InvDB:HIT000013064"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
5-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 148..1854
/codon_start=1
/protein_id="BAB71186.1"
/translation="MDTEDFPGPELPPPAAEGASSVQQTQNVKASTSSPMEEAISLAT
DALKRVPGAGSSGCRLSSVEANNPLVTQLLQGNLPLEKVLPQPRLGAKLEINRLPLPL
QTTSVGKTAPERNVEIPPSSPNPDGKGYLAGTLAPLQMRKRENHPKKRVARTVGEHTQ
VKCEPGKLLVEPDVKGVPCVISSGISQLGHSQPFKQEWLNKHSMQNRIVHSPEVKQQK
RLLPSCSFQQNLFHVDKNGGFHTDAGTSHRQQFYQMPVAARGPIPTAALLQASSKTPV
GCNAFAFNRHLEQKGLGEVSLSSAPHQLRLANMLSPNMPMKEGDEVGGTAHTMPNKAL
VHPPPPPPPPPPPPLALPPPPPPPPPLPPPLPNAEVPSDQKQPPVTMETTKRLSWPQS
TGICSNIKSEPLSFEEGLSSSCELGMKQVSYDQNEMKEQLKAFALKSADFSSYLLSEP
QKPFTQLAAQKMQVQQQQQLCGNYPTIHFGSTSFKRAASAIEKSIGILGSGSNPATGL
SGQNAQMPVQNFADSSNADELELKCSCRLKAMIVCKGCGAFCHDDCIGPSKLCVACLV
VR"
BASE COUNT 755 a 645 c 589 g 680 t
ORIGIN
1 aaatatctag agcaaagctg tccaaaggct atcaaaactg aacatgccaa ctacttgaac
61 gtgtcagaac ttcatcccag gaatcttgta acaaatgttg ctcttcctgt gaaatctgaa
121 cttcacgaag cagacaaggg ctttagaatg gacactgaag acttccctgg ccctgagctg
181 cctcctccgg ctgcagaggg agcctctagt gtacaacaaa cacagaacgt gaaagcttcc
241 acctcaagtc ccatggaaga ggctatttcc ttggctaccg atgccctgaa gagagtccct
301 ggtgcaggga gctcaggctg tcgtctgtcc tctgtggagg ctaacaatcc gctggtgacg
361 cagttactac agggcaacct gcctttggaa aaagtgttgc cacagcccag attgggagcc
421 aagcttgaaa tcaacaggct tccattgcct cttcaaacta cctcagtggg taaaacagca
481 ccagagagaa acgttgaaat tccgcccagc tctccaaatc cagatggtaa gggctacttg
541 gcagggactc tggcaccact ccaaatgaga aagcgagaaa accaccccaa aaagagagta
601 gctaggactg taggagaaca cactcaagtt aaatgtgaac caggaaaatt gttggtggag
661 ccagatgtta aaggggtgcc ttgtgtcatc agttccggca tcagtcagct aggacacagc
721 cagccattta agcaagaatg gctaaacaag cactccatgc agaacagaat tgttcacagc
781 cctgaggtca aacagcaaaa gcggctgctc ccctcgtgta gcttccagca gaacctattt
841 catgttgaca agaatggcgg cttccacact gacgctggta cctcacacag acagcagttt
901 taccaaatgc ctgtggctgc caggggcccc attcctactg cagctctgtt acaggcctct
961 tccaagaccc cagtggggtg taatgcattt gccttcaaca ggcatcttga acagaaggga
1021 ttgggagagg ttagtctttc ctcagcacct caccagctaa ggttagccaa catgttatcc
1081 cccaatatgc ccatgaaaga aggtgatgag gtgggaggca ctgcacacac aatgccaaac
1141 aaagcactag tacatccgcc gccgccaccg cctccccctc cccctccacc cttggctttg
1201 cccccgcctc cccccccacc acctccgcta cctccacctc tccctaatgc agaagtccca
1261 tctgatcaaa aacaacctcc agttaccatg gaaaccacta agagacttag ttggccacag
1321 tccacgggca tatgtagcaa tataaaatcg gaacctcttt cttttgagga aggtttaagc
1381 agcagctgtg aactgggcat gaaacaagtt tcctatgacc agaatgaaat gaaagaacag
1441 ttaaaagcat tcgcgctaaa aagtgcagat ttctcttcct atttgctttc tgagccacaa
1501 aagcctttta cccaattagc tgctcagaaa atgcaggtgc agcaacaaca gcagctctgt
1561 ggaaattatc caacaataca ctttggtagc acgagtttca aaagggcagc atctgcaatt
1621 gaaaagtcca ttgggatttt gggaagtggc tccaatcctg ccacaggctt gtctggtcag
1681 aacgctcaga tgcccgttca gaactttgcc gacagcagca atgcagatga attggaactg
1741 aaatgctctt gccggctgaa agccatgatt gtgtgcaaag gctgtggggc cttctgccat
1801 gacgactgca taggtccttc aaaactttgt gtagcatgcc tggttgtacg ataagagctg
1861 agtgaaagat gcagtatccc ttttccacac ggaaaagcca aatagcatca gcaacaacaa
1921 atagaataat gcagtggttt ctatcatgct aatttatttt gctttggagc aggtaccttt
1981 cctctatggc attattttct tgcatttctc ataaagggga ggatgcatcc caactgaatg
2041 gctcactggc atgtctttta tgtgttcagt tgccattcct agctttggaa agtttctatc
2101 tgtccatgtg tatacaagtc aatgccccat ttttgttttt cttttaaccg aggtgtagat
2161 aggaaaagga catttttaat tacttaaccg gaaatgcaga tgtgtaagga gaatgagagg
2221 aatgagttaa agtggttatg catttttcta tagatgagcc attacagcaa ggaattttac
2281 agttgacttc tctgaaccta gctttaccac agtgattaaa tcctatttag aaaggggaat
2341 ctgatttaaa tgtgtgattc cttgtatttg ctcctatcac aaaagatata ttaaaggagg
2401 tatgccatta atgaaatcca ctgtcctgag tattatcttt cctcctgttg tactttctca
2461 gagactatgg cagaatatct ggatcttcct tggattttgt acacatattg tagtgaaatg
2521 tgtcctacat ctgaaattgc atgggactca tgcccagcaa tctggttcta ggcctttgac
2581 acctgataat atgagagcaa ttggccagcc aatagccata agcccagagg attttagagc
2641 ttcatgtgtg gcttttaaga gcaggtttg
//