LOCUS AK056357 2082 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31795 fis, clone NT2RI2008812.
ACCESSION AK056357
VERSION AK056357.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2082)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2082
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008812"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012971"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 10..684
/codon_start=1
/protein_id="BAB71162.1"
/translation="MAAPSEVAAIAPGEGDGGGGGFGSWLDGRLEALGVDRAVYGAYI
LGILQEEEEEEKLDALQGILSAFLEEDSLLNICKEIVERWSETQNVVTKVKKEDEVQA
IATLIEKQAQIVVKPRMVSEEEKQRKAALLAQYADVTDEEDEADEKDDSGATTMNIGS
DKLLFRNTNVEDVFNARKLERDSLRDESQRKKEQDKLQRERDKLAKQERKEKEKKRTQ
RGERKR"
BASE COUNT 673 a 370 c 491 g 548 t
ORIGIN
1 gaccccaaga tggcggcgcc cagcgaagtg gccgcgatag cccctggcga aggcgatggc
61 ggaggcggcg gctttggctc ctggctggac ggacggttgg aggcactggg agtggaccga
121 gccgtttatg gagcctacat cttgggtatc ctgcaggagg aggaggaaga agagaagctg
181 gacgctctgc aggggatcct ctctgctttc ctggaagaag attccctcct taatatctgc
241 aaggagattg tggaacgatg gtcagaaact cagaatgttg tcaccaaagt gaaaaaagaa
301 gatgaagtac aggccattgc caccctaatt gagaagcagg cacaaatcgt agtaaagcca
361 aggatggtgt cagaagagga gaagcagaga aaagctgccc tcctggccca gtatgctgat
421 gtgacagatg aagaggatga agcagatgag aaggatgatt caggtgctac cacaatgaac
481 attggttctg acaaacttct gttccgaaac accaatgtgg aagatgtctt taatgcccga
541 aaactggagc gagactcact tcgggatgaa tcccaaagga agaaggaaca ggacaagctg
601 cagagggaga gagacaaact agccaagcag gagcgcaagg aaaaggaaaa aaaaaggaca
661 cagagagggg agcgaaagcg ataaccttgg cctactctat tctttccccc catgaactta
721 tcaaagggag aactggttgt gtatttaaga gaaatgagag tgcattgcaa agtagtttcc
781 caaggcattt cccctattgt ttacatggtc aaaaggaaaa tcaaaatcac ttctaattac
841 aaaatgtgct gttttggtgg ggtgggcaat cagattatta tagttgatga ctgtaccaaa
901 gatctggaat gggggccacc tttatatttt aatactcaag tcctgtgctc cttttggcca
961 ctttaaaaag tcttccctca ccttggataa ataggattca gaaagctaac agttttcatt
1021 ctgaaggagc agaaacataa tcaatccctc cctgagataa aatgtgggca tgcacattgc
1081 tttggagtca gtattcagct gtaagtgtag tattgtttta tggctgaaat agaaagctaa
1141 tgaagaggcc tacctggatc cagattttat gttaacatgg cagcaaaact aggaaaatgg
1201 gaagaaatta cttgcggggt gttctagatc tgattttttt tttttttttg aaagtttcag
1261 cctgtgggag gaaaagaggt atcttttaaa atagagatag ttacatattt tcctcatcta
1321 ggctgtgtca ctattgggtg caaggatatc attgttggct agattcattt tgtataatca
1381 tgtatcctct tgtgtgctgg tagagatttt aatcctgatt tttccataaa acatgagtat
1441 taagaaataa ttcctggttt ggagaaactg gagaaatcac ccttttaagg aagaaacact
1501 ggaaatttct gctaacacca agatatttaa gagtgtacat agtaggtgct caacaaattt
1561 attgaatgaa tgagtgaatg gaaaaactgg gagagtcaaa agtgagcaga agctctccat
1621 ttctacttct gtcacaaacc acattaaatt gtaaataagg cccttctcca cttgacttca
1681 ggcagcagat tgtctagaag cctaaggaca gcaatttctc tgacaagaca aagtagatat
1741 tttataccag gggttggcaa actactgccc acgggccgaa tttggcccag tctgtttttg
1801 tatggtgcaa actaaaaatg atttttacat ttttaaagag ttataaaaga aaaaaatatg
1861 tggtctgtga aatctaaaat atttactacc tggcctgttg gaggaaaggt ttgccaatct
1921 ctgttttata ccattaacta tgagattaac aaaaactttt acctttgtgc agaaggttaa
1981 aaaaaaaaaa acatggttaa ggaaaaggag acatgttacc tcttcataca ctcctataac
2041 tgtggcattg caaaaaataa aaataaccac ctttaaaaaa at
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