LOCUS AK056350 2020 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31788 fis, clone NT2RI2008598, weakly similar
to MILK FAT GLOBULE-EGF FACTOR 8 PRECURSOR.
ACCESSION AK056350
VERSION AK056350.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2020)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2020
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008598"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012964"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 494 a 519 c 530 g 477 t
ORIGIN
1 agctagccgg agccgcgggt gagcgcggcg agcggcgacc ctggtgagga gcgcggcgcg
61 ggaggcacgt tccttagctc cgccgcggcc gtcctccgcg gctcgaggac tccgcttcct
121 tccctcccct cccctgcgct ccggcctggg gtcttggcgc ggggagcgga gggaagggac
181 gaaggaggag taggtgaaag cggggtgagg ggcggaaggg tcccggcgcg gggtgaggcg
241 agggctgcct cttgttctcc cgccgctgcc gccgtctcct ggtcgggtgc cgcggccaga
301 ggcgcgcggg gctgccgagg cacccgcact atgcaggcag actgccggcc gccgcgatgg
361 cgagccgggc ggtggtgaga gccaggcgct gcccgcagtg tccccaagtc cgggccgcgg
421 ccgccgcccc cgcctgggcc gcgctccccc tctcccgctc cctccctccc tgctccaact
481 cctcctcctt ctccatgcct ctgttcctcc tgctcttact tgtcctgctg ctgctgctcg
541 aggacgctgg agcccagcaa ggtgatggat gtggacacac tgtactaggc cctgagagtg
601 gaacccttac atccataaac tacccacaga cctatcccaa cagcactgtt tgtgaatggg
661 agatccgtgt aaagatggga gagagagttc gcatcaaatt tggtgacttt gacattgaag
721 attctgattc ttgtcacttt aattacttga gaatttataa tggaattgga gtcagcagaa
781 ctgaaatagg caaatactgt ggtctggggt tgcaaatgaa ccattcaatt gaatcaaaag
841 gcaatgaaat cacattgctg ttcatgagtg gaatccatgt ttctggacgc ggatttttgg
901 cctcatactc tgttatagat aaacaagatc taattacttg tttggacact gcatccaatt
961 ttttggaacc tgagttcagt aagtactgcc cagctggttg tctgcttcct tttgctgaga
1021 tatctggaac aattcctcat ggatatagag attcctcgcc attgtgcatg gctggtgtgc
1081 atgcaggagt agtgtcaaac acgttgggcg gccaaatcag tgttgtaatt agtaaaggta
1141 tcccctatta tgaaagttct ttggctaaca acgtcacatc tgtggtggga cacttatcta
1201 caagtctttt tacatttaag acaagtggat gttatggaac actggggatg gagtctggtg
1261 tgatcgcgga tcctcaaata acagcatcat ctgtgctgga gtggactgac cacacagggc
1321 aagagaacag ttggaaaccc aaaaaagcca ggctgaaaaa acctggaccg ccttgggctg
1381 cttttgccac tgatgaatac cagtggttac aaatagattt gaataaggaa aagaaaataa
1441 caggcattat aaccactgga tccaccatgg tggagcacaa ttactatgtg tctgcctaca
1501 gaatcctgtg cagtgatgat gggcagaaat ggactgtgta cagagagcct ggtgtggagc
1561 aagataagat atttcaagga aacaaagatt atcaccagga tgtgcgtaat aactttttgc
1621 caccaattat tgcacgtttt attagagtga atcctaccca atggcagcag aaaattgcca
1681 tgaaaatgga gctgctcgga tgtcagttta ttcctaaagg tcgtcctcca aaacttactc
1741 aacctccacc tcctcggaac agcaatgacc tcaaaaacac tacagcccct ccaaaaatag
1801 ccaaaggtcg tgccccaaaa tttacgcaac cactacaacc tcgcagtagc aatgaatttc
1861 ctgcacagac agaacaaaca actgccagtc ctgatatcag aaatactacc gtaactccaa
1921 atgtaaccaa agatgtagcg ctggctgcag ttcttgtccc tgtgctggtc atggtcctca
1981 ctactctcat tctcatatta gtgtgtgctt ggcactggag
//