LOCUS AK056349 2005 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31787 fis, clone NT2RI2008566.
ACCESSION AK056349
VERSION AK056349.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2005)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2005
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008566"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012963"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 554 a 456 c 571 g 424 t
ORIGIN
1 attacaccag ctctctcagt ccggggagga ggaggagcag gaggaacgcg agggggaagg
61 agaggaggag cggccagcag tagccacgac cgccaccacc aggcagagga agagttcgtg
121 gggaggaaaa gacctctcct cccccttgga gcgccttctc tctgcttttg gagaaaggga
181 atacgcgata aagaaggaga cggaaagaag agaaaaagtg aggcgggaga cagagggaaa
241 agggcgtgaa cagaaagggc cggagcgtgc aggggagcac agggcgcggg ggcggcactg
301 ccgagccggg agcgctgccg cttgggcagg tgccgcggcc gctgcccgga ggcggcatgt
361 gacgcgcggc cgcagctgcc cgcgggcgga gcgctctcag accccggagc gcacaccgcg
421 gggccatcgg tgccatcgcg gatctccagg ctcctcatca gtccgccggg gccgcagcag
481 cgcttctggg aagacgggcg atgaactgaa gggcggctcc gggcaggggg gcacgatctt
541 aaggacagtc gctccctgaa cgcggagccg gaggagacga agggaaggtg gagcggacgc
601 cacccgcgca ccgggcaggc gcggagaccg gcgtgggaca gccacctgga gcgcagctgc
661 cagaaagaag gactttgctg ctttgggcca ggatctgaac ttaggtgtaa accattgccc
721 tggcagaggg aacctaccca gtccattgct gcctgctaca agatatgaac agtaatggca
781 catattttgg ttatgagtca ctcagtggac tgtggattga atgaactgta tccatcccca
841 tcatgatgta cagaaccaag tctcttcact acacttgtgt tctgattagt ggccaaataa
901 atgacagtca gaacttcagc taaggtacaa taaggttttg ctgtggcgtg gcacctggtc
961 tctttctaga aggaaagttc aacatacagc acaattctgg acacattgta ttggtttgat
1021 gctcctacct ggaacagatc ctcacgagct agtaagtttg aaggggagtc ataaagagga
1081 acaggcatta tacgcaaggc tgaagaagca gttggtaact aacagtacat ctttttctaa
1141 ggccaacact aaacataagt tgcagagctg gcctgtggtg gaactcacaa gtcttattgg
1201 caatatatgt gaggagacaa aaagcaacat atggagaaat acaaaaatat actccctaga
1261 gatactgcct cttcagatgt caagaaaaac ctggatgaaa tgaagcaaga gtggcacgct
1321 gcaaagttac aaattcaagg ccacccacag aaccaaacat taacacttag gtggagctgg
1381 tctcttgggc agcctgacat ctggtccact cctctgtcct atgccgggat catcatgacc
1441 gtagagatta tgtcactgtt ttgcatcctt gttacataac tcagttcctg gtagattcca
1501 gtgaaaggca catggtggtg atatttttga gaaagtagag aagaaaagaa tgaatccgga
1561 tggttgttga gacagaaact gagcgtcgaa cctcattttc tctcgtaccc aaaccttgat
1621 gaaacaacag aggaaagtat tgggctgcaa ttgtaggcgc acaaaagcaa accttgagtc
1681 atggatgacc gaccaaggaa gagtcccctt gggaggagga ggtgccaaag accagtgata
1741 ctgctttggg ttatgtgcac tgagggtttc caaccctgac ggatatggat tctaatttaa
1801 aatatcacag ggaagaaaaa aatagacgtt cccatggcaa cattttctca gggccagctc
1861 ttctgtctta ttatcatatc ttatttaaat atttcctgga atcagtttaa atttaatccc
1921 tctgtgtgtc ttctattttc agtggccatg gagagcgtaa catctggtca gaggttttcc
1981 ttaaataaat catataattg taggc
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