LOCUS AK056348 2182 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31786 fis, clone NT2RI2008526.
ACCESSION AK056348
VERSION AK056348.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2182)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2182
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008526"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012962"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 347..709
/codon_start=1
/protein_id="BAB71159.1"
/translation="MAILVASLIRNRRGAKEASCVGPQSESGNDFKLKRRRRAEFPFL
NGLISELGWSLTGSAGEGRVGTHCPFALPPPRAEADSAGARVAELGERKCPSQSEVAG
PAPRLRERRSSWVRLCLL"
BASE COUNT 601 a 423 c 624 g 534 t
ORIGIN
1 agaaccggaa aacaaggagt aacagtcgcg tttcaaatct cgcagacctg ctggccgatt
61 tcgagtcttc tgaaagagga aggcgagcaa agccaaaatt ctcccagagc gcgaacccaa
121 acacagatca ccgctggggt ccaagcgccc ttcaactccg cccgctagtg cccaagatta
181 ccgggaagga attcaacgaa tcagtggtga ggtgccgccg ccatgcctac tacgggcgga
241 aatttgagag aaagcttcgc accggtagga aaactgagga gcaaccgagc acgattggaa
301 aggttgacga tagtggttaa gtggctcggc cttgccctca gttaaaatgg caattttagt
361 tgcttcactg atacggaatc ggcgaggcgc caaggaggct tcctgcgtgg ggccgcagag
421 cgagtcggga aacgatttta aactgaagag gcggcggagg gccgaattcc cttttctcaa
481 cggcttgatt tcagagctgg gctggtctct gacaggctca gctggagagg gacgggttgg
541 gacgcactgt ccttttgccc ttccccctcc gcgagcagaa gctgactccg caggagcgag
601 ggtcgcagag ctgggtgagc ggaaatgtcc ctcccagagt gaagtcgcag ggcccgcccc
661 gcgtctgagg gagcggaggt cttcctgggt gcgtttgtgt ctcttgtgaa cccacttgtg
721 gagcgagagg gaatttggga gggccagggg ttagtctggc ctctgtctta gctgtcattt
781 tggcggtttt tttttttttt tcttcagggg atttcagtct ccacatagtt ttggagccgg
841 acttttgaag aatgattcgt gagtccggaa tgggtgacag cgtcatcacg gcattttatt
901 ggtaaagatg ctttgtattg gtcccggacc tgctttaact ttgtatttgc cctgttgtgt
961 ggtcattatc ttacaatact tgcgtgttga ggtgtgttta aacactacgt gcttaacact
1021 ggctaagtat tggcattggg gcggttggat ttagctattg gtggcaatgt agaaaagtga
1081 tcaagtcaga gccccttcac ggactaatca caagagaaac aacaaagctt tacttgtgtg
1141 ctgtgaacca caactgcaag tggaattgtc aagagaacta gccctgccaa gctcaggaaa
1201 acttctgagg agatggcaca tggaaaatgg ataagattta ctgagacaaa cgggagtaga
1261 gaagtgtttt taattggcga taaggtgaat aaaaaggcag ggagaacagt agagtgatca
1321 tctgtctaga gtagggggtg catgtttggg tatagggctg tcatttaacc tcttaaagaa
1381 ttgtctactg gaactttctt cactcccatc cccccacccc ccaatgttgt atacaaaacc
1441 ttagaggaag tgtatgcaga atgttggatt ttggaaaggc taatgtcgat ggatcttggg
1501 gaagaactga agtagtagaa aatgaacctg gataggatca gataataaga tcaaatacag
1561 tagtgccttg aaagccagac aggaattctg atttgacttc atatagatgt gtgggaactg
1621 ttatgggatc tcaagcaagg taatgataaa agtggtgaca atgaaagact gatgatggat
1681 tagggaaggg ggctaagatt gggaacaaga taagtagcta taattgagaa tgaaagtgaa
1741 gcccacatct aagaggacaa gtgtatatct ttttaagaaa tatgtcagag gccaggtgtg
1801 gtggcttatg cctgtaatcc agcactttgg gaggccaagg caggcggatc acctgaggtc
1861 aggagttcaa gaccagcctg gccaacatgg agaaacccca tctctaccaa aaaatacaaa
1921 aattagccag gcgtgttggt gcatacctgt agtcccagct actggggagg ctgaggcagg
1981 agaatcactt gaacctggga ggtggaggtt gcagtgagcc aagattgcac cactgcactc
2041 cagcctggga gacagagtga gactctgtct tcaaaaaaga aatatgtgag aatttgatga
2101 taaagttaac aaagttaact ttttagatgt aaaggtgatg ttgaaggaag agtaaaagat
2161 gatgaaagtt tttttagtgg gg
//