LOCUS AK056337 2673 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31775 fis, clone NT2RI2008115.
ACCESSION AK056337
VERSION AK056337.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2673)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2673
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2008115"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012951"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 696 a 639 c 644 g 694 t
ORIGIN
1 ctgaaagtag agtgtctgag aggacagaag gtaatgggaa ctgtagctgg aggcctcagg
61 ccatgggtca aacctgggag ggaaagagac cctacacatg gcctagaaat gagagaagag
121 agaggtattt acccagagga ttttcctatg gttggggatg caaatattag aaaacagatt
181 gtattttgct gaggggagtg gctgtcatga gcatgtcagt tctaaaaggg gttttcatta
241 tcctggaaat gtataaacta aagtaagctg attggctttg caaacatgtt catttgtttt
301 tcagacagta tgggttaagt tctctgccct ccccaggggt ctgaggaggc tctgggtttc
361 tcagatctgt ctcttgctgc gttttcacat cagctgtgct gcttggtgcc tctctgatac
421 gaatacactg acacgtcaaa gtaacctaat gtggacacca tccagaaaac tccagttcat
481 gctggatctt aaccaaaaat gattcaatac tgttatcact aaaacagcac caagacctga
541 agccatcttc ccttggagtc aactgactac cacctctata agcctagtca atgagcagac
601 cccttccagt atttgtaaaa gtagtactag gttgcctttt tggcaatttt tattgacctg
661 ttgaatcttg actataaaat gatctgagaa gtaaggaagg ctgggctgat gtgtggctct
721 catatacctt ctgcaagggg gcagtctccc cagctccctg atgatgctca cccccgcccc
781 cccacctcag gtgctgctgg tgtgagccaa agactggagt ttttccagct ggggtgggag
841 tggagagaca acaggaacaa cgctgcacca aagaaaaggt cagaataaaa ggcagcacag
901 ctggtgacct tattttctag atgttacaaa tcaggtcact atgcaaacta gaatatcctc
961 agcaggtggc ctggccactc tggagaaaga aacccaagga aagtgagcac ccaactggat
1021 gccaagacac ccgggttctg aaaatgtgct gtgttcctac ctcggcaaga tcaccagcac
1081 tgaggggccc agctggagaa tgattctgct acaaaaggag acagttgaga cttttgcttg
1141 ttggaaatca aacttcttat ttgtctaaat tgcccctttt tctgttccta aaaggaagga
1201 taagagagaa cattccaggt gaggcacttc aaagtttcct tagaccctat agtgttaaga
1261 ggtattttaa acactaaaag gacaaagctc ttcccaatcc ttatgcttcc ctaagtggta
1321 tctgcagcag tttgttgtgt gcagtttgat ggcagctgca aactgtaggt gaggcggagg
1381 aaaggcaggt aggaaggagt aaggatggag atgctcagaa tcaagagcat ggcggagtag
1441 gagaagaagc cctgcacaca gggcagtgtc cacagccaga aaactcctgc tgggcaccaa
1501 ccactacgag cataccccat gcccaccgtg gagctgcaac tcctcgacag cactgagttt
1561 gatagtctca ctggaagcag atcagctgat gtagaacaga gacctcggcc ataaaggtga
1621 gaagacatag ggatttcaac cacacagttg ggacagaagg gacagtgcat ctgttcatcc
1681 atcctgcact tggcccacgt tgaactccat ggtgcctgag agagactagt taagggttgg
1741 tcttctgtat cctctgctgt tgagcctcta gtaagctttc atctcccatg aactcatttc
1801 cccataaatg aaatgggtaa ataatgcccc atttgtagaa gtgggccctc atgactgagg
1861 tagcttccag ataggccaga gtagagtgta gagtgtgccc cgtgacatcc ctccatcttc
1921 tcctccatta tcatctagca gggtcagact gggaaacctg gttggccacg ccacaccatg
1981 accgaggagc caactgggac ttctggctgt ttgacatcct catgttcccg ttggtcttcc
2041 ggagaatagt gctaccctca catcccctgg agcacagcct tcctgaaatg ccctcacccc
2101 atgcctttgc cattgtgtgc tctcagattt cttccactgt ttgacaccct ctttagaggg
2161 ctgctctttt ttttccagag ataatcctag ccatcctctc cactcccacg gctggggaca
2221 atggccactt actacctgtg cactttgcca ctcgggacac ctggatggtt tctcttagga
2281 ctttgcccac ctccttctca tggcacttgc tgtggaaaat gcctggctgg cctcgtgggg
2341 cctgtctcac ttttccagga gacatgaccc actaacgtgg caactttaac ccaaaggccc
2401 ctcagacatg ttacagcaaa tctggagcca cagacaggtt ccctccattg gcagcccatt
2461 gtgtttgaaa ttccatgtcg ggtttacttg gaatgaaaga tacttgaatt attgtgcgcc
2521 tgtgagcgcc cagcttctgt ttcatagtct taacaggtgg ccattgtcgt gaaacgagtg
2581 atgcctgaag atctcagtga tgtttgaacc ttctgtgtaa ctttttatta agtctttgta
2641 tctctcgact gattaataaa gaagagaaac acg
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