LOCUS AK056312 1882 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31750 fis, clone NT2RI2007406.
ACCESSION AK056312
VERSION AK056312.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1882)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1882
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2007406"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012926"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 461 a 487 c 452 g 482 t
ORIGIN
1 cggtgtcaag accctcagag tctttgagga aagagccaag cagagaagac cgctgcgtgg
61 agtgtgacac cacatggcat ttctcaagcc cagcggaccc tgtgtcagca aataccagta
121 atttaacatc tgctcctggc ctcctcttca cctgcctggg ttcggaactg aggaggaagg
181 atgagaagag actgcaatgc agcaggcacc ctctgcagag tgaggccgcc cgtccatcac
241 tgccagccct gggctgctca ggaggggacg tgaaccactc aaaggaatgt cttaaagagg
301 atctggagtt tcccccatgc aaattaggga agaattaggc aagaaaagac atacattaca
361 gggaaatcct tctgaacaaa atggctgtct tcacctactc ttcttgcaag aaaagacata
421 cattacaggg aaatccttct gaacaaaatg gctatcttca cctactcttc agcaaaaacc
481 gtctccagac tttttagtgt caaaaatgta accaactgtc tcctgatttt ttccaatgtg
541 tttggcaagt gctgtggccc tgttgtaggt acttctcctg actcttgggg gagaagtggt
601 tttagggatt gatttggggg agcgggtttt cgtcccatcc cacctcctct cttctttctc
661 tgcttgtcga tattgtctgt gtgattcaga gaattggggc agttacattg tgtctgttgt
721 tgagattatt aaaagattaa aacgtagttg tagaaatgaa aaattaaaca gctgtgtttt
781 aaaaatgcaa accaatatct tgttaataaa ggaattcaag ctatggggca gccacactcc
841 cctcctccca ggctggcata ggtggccctg ggctggcggc ttaggaagca tggagcacac
901 ttagggtagt gcctgcctgg gcaagagaca cctgccgagc aaaaggaacg agaatctggg
961 gctcagagcc tcgcagttgt gccaggattt tcctatacat gttcaggacc tctgggtaga
1021 ggtttcacag tttgtgaccc cataaccacc ctcacccgac gtgggttcta gctcagtgtg
1081 cctaatactg catggtaacc tgggcttgac cctgaagccc ctgcctggca ggccacagga
1141 cctctcctgc ccacttggtg ctatctcttc cagtgaccac ccagcacggg tgagcactgg
1201 ccagcctgga gcctgcaatc caggtggaac agactgtcct ccatgtcagt tccttctggc
1261 ttcaggccct caattctttc cctttgagct tttttagacc ccagatctcc taggcccagg
1321 ctctctcttg accccagaga agccactgtc aggaaaggaa gtgaacccta ctgaagccag
1381 agaattcacc ccggccaaag caggccctct gggtccagcc cctcattcca caccacacca
1441 gtattgcatc catctactgc agctacacat cctgagggca gcaccaccca ctctggcctg
1501 ctggcccatc gcaggactag cccaggcacc tgccgggcat tgcaggatat ccagtggggc
1561 ctgtgactgc tccctgatgc gtcagaagag aagtgttgca ctttagtgga ggagctgagg
1621 agcacctgcc cccttgtagc ttgagttcct tttggtaaca gtagcagcct ccatggtggt
1681 gtctgggatg cacgtgcacc cgctgccttc agctgtatgt gtgtgttcct gctggagttg
1741 tgttactgac aggataatga cattacaaag aaattgtgtt atattcaact ttgccttgat
1801 aattattgta aacactttgt tcattttttc ttttttattc acaaactaaa tccaccagga
1861 aattataaag ttatttaaaa ac
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