LOCUS AK056284 2374 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31722 fis, clone NT2RI2006679, highly similar
to Homo sapiens follistatin-like 4 (FSTL4), mRNA.
ACCESSION AK056284
VERSION AK056284.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2374)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2374
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006679"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012898"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 272..1747
/codon_start=1
/note="highly similar to Homo sapiens follistatin-like 4
(FSTL4), mRNA"
/protein_id="BAG51665.1"
/translation="MGANSTSAVFGMKTQGHTPALPKMKWVWMKISPRSSLKTQLERP
LQTSCGERKELHLVPAPDASTWPVSCVFNAACDPAQGPTAWRACPFHLLLPGLSVGNM
FYVFSDDGIIVIHPVDCEIQRHLKPTEKIFMSYEEICPQREKNATQPCQWVSAVNVRN
RYIYVAQPALSRVLVVDIQAQKVLQSIGVDPLPAKLSYDKSHDQVWVLSWGDVHKSRP
SLQVITEASTGQSQHLIRTPFAGVDDFFIPPTNLIINHIRFGFIFNKSDPAVHKVDLE
TMMPLKTIGLHHHGCVPQAMAHTHLGGYFFIQYRQDSPASAARQLLVDSVTDSVLGPN
GDVTGTPHTSPDGRFIVSAAADSPWLHVQEITVRGEIQTLYDLQINSGISDLAFQRSF
TESNQYNIYAALHTEPDLLFLELSTGKVGMLKNLKEPPAGPAQPWGGTHRIMRDSGLF
GQYLLTPARESLFLINGRQNTLRCEVSGIKGGTTVVWVGEV"
BASE COUNT 564 a 689 c 607 g 514 t
ORIGIN
1 acctacttgg gttcaccccc tctggagcag ggtctcaggt tgggccacag gcccaagtcc
61 aggtcagggc cgtctctttc tcaccagcac tgactctcac ccacagtgcc gccagtcatc
121 cgtgtctatc cagagagcca ggcacaggag cctggagtgg cagccagcct aagatgccat
181 gctgagggca ttcccatgcc cagaatcact tggctgaaaa acggcgtgga tgtctcaact
241 cagatgtcca aacagctctc ccttttagcc aatgggagcg aactccacat cagcagtgtt
301 cggtatgaag acacaggggc atacacctgc attgccaaaa atgaagtggg tgtggatgaa
361 gatatctcct cgctcttcat tgaagactca gctagaaaga cccttgcaaa catcctgtgg
421 cgagaggaag gaattgcatc tcgtgcctgc accagatgca tccacgtggc cggtttcttg
481 tgtgttcaat gctgcgtgtg accctgccca ggggccgact gcttggaggg catgcccatt
541 ccatttgctc ctcccaggcc tcagcgtggg aaacatgttc tatgtcttct ccgacgacgg
601 tatcatcgtc atccatcctg tggactgtga gatccagagg cacctcaaac ccacggaaaa
661 gattttcatg agctatgaag aaatctgtcc tcaaagagaa aaaaatgcaa cccagccctg
721 ccagtgggta tctgcagtca atgtccggaa ccggtacatc tatgtggccc agccagcact
781 gagcagagtc cttgtggtcg acatccaagc ccagaaagtc ctacagtcca taggtgtgga
841 ccctctgccg gctaagctgt cctatgacaa gtcacatgac caagtgtggg tcctgagctg
901 gggggacgtg cacaagtccc gaccaagtct ccaggtgatc acagaagcca gcaccggcca
961 gagccagcac ctcatccgca caccctttgc aggagtggat gatttcttca ttcccccaac
1021 aaacctcatc atcaaccaca tcaggtttgg cttcatcttc aacaagtctg atcctgcagt
1081 ccacaaggtg gacctggaaa caatgatgcc cctcaagacc atcggcctgc accaccatgg
1141 ctgcgtgccc caggccatgg cacacaccca cctgggcggc tacttcttca tccagtaccg
1201 acaggacagc cccgcctctg ctgcccgaca gctgctcgtt gacagtgtca cagactctgt
1261 gcttggcccc aatggtgatg taacaggcac cccacacaca tcccccgacg ggcgcttcat
1321 agtcagtgct gcagctgaca gcccctggct gcacgtgcag gagatcacag tgcggggcga
1381 gatccagacc ctgtatgacc tgcaaataaa ctcgggcatc tcagacttgg ccttccagcg
1441 ctccttcact gaaagcaatc aatacaacat ctacgcggct ctgcacacgg agccggacct
1501 gctgttcctg gagctgtcca cggggaaggt gggcatgctg aagaacttaa aggagccacc
1561 cgcagggcca gctcagccct gggggggtac ccacagaatc atgagggaca gtgggctgtt
1621 tggacagtac ctcctcacac cagcccgaga gtcactgttc ctcatcaatg ggagacaaaa
1681 cacgctgcgg tgtgaggtgt caggtataaa gggggggacc acagtggtgt gggtgggtga
1741 ggtatgaagg gcccagagca gagccctggg ccaaggaaca ccccctagtc ctgacactgc
1801 agcctcaagc aggtacgctg tacattttta cagacaaaag caaaaacctg tactcgcttt
1861 gtggttcaac actggtctcc ttgcaagttt cctagtataa ggtatgcgct gctaccaagg
1921 ttggggtttt ttcgttagga agtatgattt atgccttgag ctacgatgag aacatatgct
1981 gctgtgtaaa gggatcattt ctgtgccaag ctgcacaccg agtgacctgg ggacatcatg
2041 gaaccaaggg atcctgctct ccaagcagac acctctgtca gttgccttca catagtcatt
2101 gtcccttact gccagaccca gccagacttt gccctgacgg agtggcccgg aagcagaggc
2161 cgaccaggag caggggcctc cctcccgaac tgaaagccca tccgtcctcg cgtgggaccg
2221 catcttctcc ctcgcagctg cttcttgctt ttctttccat ttgacttgct gtaagcctga
2281 gggagagcca acaagactta ctgcatcttg ggggatgggg aaatcactca ctttattttg
2341 gaaatttttg attaaaaaaa aattttataa tctc
//