LOCUS AK056281 2142 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31719 fis, clone NT2RI2006659.
ACCESSION AK056281
VERSION AK056281.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2142)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2142
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006659"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012895"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 469 a 548 c 580 g 545 t
ORIGIN
1 cagagcaggg gatagaagga cagatgtctt caccctcatg gagttcacct tccagtagga
61 ggaggcgata ggccggggtc tgcacatgtg cgtgctacag cctgttccac ggtgcgtggc
121 gtgcggggca gtagagacag gatttcacca tgttggctag gatggtctcg atctcctgac
181 cttgtgatcc gcccctccct cagcctccca aagtgctggg attacaggcg tgagccactg
241 caccctgcca gaaaactcat tcttctactc catcctacag ttttccctaa gagagaaaca
301 ataaaacgcc accacgacca atggcaaaaa gctggcaccc actccacgac ttttcatatc
361 tacacgtttg tacagcttta tttttaagca ttctgaaatt ctatgcagga gagaccccag
421 ctaggtttag ggagtcctag ggtttgtgga gtaaatgaag tttctcccta gaattaggga
481 gggtagagac aggcagagaa ctgacaatcc taacagctgc tgtcctcaga gccactgttt
541 ctgagagctg ctcgctgagt gcttctagcg agttaaatgg tgttcgccca aaagacctgt
601 tcacgtcctg atcctgggaa cctgtggctg tgatcttatt tggaaaaagc tctacattac
661 gtctctgcag aagtaatcat gttaaggatc ttgagaggac gtgaccctga attatccggc
721 tgtgcttgac atccaatgac tggtgatgtt gtaagagaaa gacggaggga gatgtaagat
781 atgagagaag gccacgccga gactggagtg atgtggccgt gagccgagga atgcctggag
841 ccaccagaag ctggcaaagg cagaaggagc ctcctctgga ccctgtggtg ggtacgcagc
901 ctggcagata tattcatttt ggacttctgg cctccagggc tgtgagagaa tacatttctg
961 cagttttaag ccacgcaatc tgtgtccctg ggaagcccaa atagggcgag accttttgcc
1021 aagtggtctc caagtgtcac gtcatcgaat ccttctcccg ggcttgtgcc atagtctttc
1081 cactttagag aggaggaaac ggaggctctg gggcacaaag ccagtcagtg gcggggcctg
1141 actttcaacc cagcctgcat ggggtcagag aacccacttt ccccatgggg cctgcggcct
1201 atgctaagga tgcttgttca tctctcctgg gcccgggagt ggttcttctg gcctagaagg
1261 caagagaagc cagtctttcg gtttcaaggt ttcccattag tggagtcaag caaaaatggt
1321 gtgttgcgct tcttcctgag ctcagcctgg gagcacggcc ttaacatgct cagtggatcc
1381 caagacggca gcatggcggt gccagcctgg cagccttagc tccttgcagc tgtgcttgtg
1441 aagggagcag tgagtggctt ccctctgtga ccaccttggg tcctaagttt cactggggct
1501 gggatccatg cgtcttgcaa ttggctagga atttcccggg ctttccctcc cttccctgtt
1561 cagggcactg ggtgtgaggc attgcatccg ttcttctgct cacctgcttc cccctaagag
1621 tgtgagctgt ataaaggcag gaaccaaaca ggagcctcca cgtgttccca gttcaagggc
1681 agtgtccctt gtgtgtttct tcaataattc agtatgactt attctgcacg gacactgcac
1741 acactcggcc ctgccgtctc cggagctggg aggtgtggag ctggctcctg acctatttac
1801 acaccgagga gggatgtgga aaacaggagg agtcccaggg ctccaatgca aagaggagcc
1861 tcttcattcc ctctgccgtg gccgtgcaag ggacagcgcc ttgtgggatt gtgtcctcca
1921 cccaattatc cttagcatta gtttgctaag gataatggcc tccagctcca cccatgtccc
1981 tgcaaaggac atgatctcat tcctttctgt gtctgcatag tattccatgg tgtatatgta
2041 ccgcgttttc tttatcgagt ctatcattga tgggcgcttc agttgattcc atgtctttgc
2101 tactgtgact cgtgctgcaa tgagcattcg cgtgcatgcc tt
//