LOCUS AK056277 2383 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31715 fis, clone NT2RI2006553.
ACCESSION AK056277
VERSION AK056277.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2383)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2383
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006553"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012891"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 644..1135
/codon_start=1
/protein_id="BAB71135.1"
/translation="MAQRTYGPECQRNLKGESPGSPGRDGYGDFFTPGTAGPGLAVSS
LRLWASPDTRPEGVGVAAAASLSSGCHWLASAPHPAPGAAAATTTAAGELCAKGACAA
YVELTEANYGAPDGTSTMSGPAHARSAGRFRTRCTGGRKVRSCLISLRAETAVAAQNR
SVG"
BASE COUNT 550 a 652 c 625 g 556 t
ORIGIN
1 aggtcccacc gctcctacca gcagcctgac ccaggatggc tctactcccc agccttcaat
61 ccctggacct gggtttggaa ttaggagact tccgtcgagg gtcaattgtg cgggatcctg
121 gccattccat acaaaatctc acgactggca aaagagaggg ggacacctcg agcagaggtc
181 agatatcaca agggacccgg gtttttccac cccgccctca ttcccagcgt ggggtctcag
241 atgagtggga aaacctcaaa tatggatcag aaatcatgca agttcggact ctggccttag
301 gagaaaactc ttaacggaga tggaatgtca cgagggatcc agccaccccc atccactcat
361 tgactggatg aagttttgcg atgggaaaat ccaggtcagg agttacggac gacctgcacc
421 gcccggagct ccttctgggt ccggagtttg ggaaaagtgc gggttagggg ttaaaggtca
481 cggaagaact tccccactcc ccccgcctct ttcgtccgcg ggccggttca agaggtattc
541 ggaaccaggg gaggagagcg gcggccatcg tacctgagca ggagccagcg cctgctggag
601 ctgctgctgc tcctcgctga tcttttgctt cagtttcttg aacatggcgc agcgtacgta
661 cggtcctgag tgtcaacgaa acctgaaggg cgagagcccg gggagtcccg gccgcgacgg
721 ctacggcgac ttctttacac cagggacagc ggggccgggc ctcgccgtct cctccttgag
781 gctgtgggca agtccggaca ctcgtcctga gggtgtcggc gtcgccgccg ccgcgtctct
841 ttcttccggg tgccactggc tggcctcggc cccccatcca gccccgggag ccgcggcggc
901 gacgacaacg gcagcaggtg aactgtgcgc caagggcgct tgcgcggcct acgtggagct
961 cacagaggct aactacggag caccggatgg gacaagcaca atgtcgggtc ccgcacatgc
1021 gcgctcggcc ggtcgcttcc gaactcgctg cactggagga cgcaaggttc ggtcctgcct
1081 gatttcgttg cgtgcggaga ccgccgtcgc ggctcagaac cggagcgtgg gctagtctga
1141 acactaccct gactctcacc tgggctttgg ccctgcgaaa tcagacgggg agagtgctgc
1201 ttctatcgat ggcagcaggg ccgacgggcc caccgcaaag aacacaggga tgagcgtcaa
1261 ggatgggaga aggggagatc cagagaacct gacgcgggcc ctaattgata gtgttctttt
1321 ctttagtagc tgtagctagg attctgtgtc ttcggtgcca gaggggcccg cttcccttcc
1381 ctgaactctt ccagcgtcgg gaagtccctg ataccaggcg ccaggggtcc aaatgtttta
1441 tgccaatact gtaaaacctc aagtgtttgg acacctgctt tattcttttc ttcaccgaaa
1501 tcgtgtattt cacgaattca ttcaaaaacc tttatcaggc cttggagatg ttaaaagaat
1561 ttacagccta gtggaagaca cagattccta attagtccaa atttactcct aacacctgca
1621 atcatcctac aagatcttaa tgtttatgtg cctcatttat ctaaaaccct gaactttggc
1681 tgcctgtgaa tcctcaggtc catttcagca acccactttt gcatgctgta cctttgatat
1741 catctgaaac tgcttacatt gtctccggta tcagaatcta acagtatatg ctgatctcca
1801 cgttctagca aattttgttt accagcaata ataggagcaa cctgctctcg agagtccata
1861 ttctgatccc taaatgtatt agagccacag tggggcctgg aacactgcct taacacctgg
1921 gctaagtcct cttatatctc gtagtgaaca ccttatgtat gtaaggtgtc cccagccccc
1981 gggccacaaa cgggtaccag cccatggcct gttaggaacc gggctacaca gcaagaagtg
2041 aatctctggc aagccaggga agcttaatct gtatttacag ctgctcccca tggttcacat
2101 tatcgtctga gctccacctc ctgtcagatc agctgtggca tgagattctc ataggagctg
2161 gaactactgt gaactaggca tgagagggat ctaggttatg ctccttatga gaatctaatg
2221 cctgatgatc tgtcactgtc tcccatcacc cccagatggg accatctagt tgcaggaaaa
2281 taagctcagg gctcccaccg attctacatt atggtgagtt gtgtaattgt ttcattatat
2341 attacaatgc aataataata gaaataaagg gcacaataaa tat
//