LOCUS AK056271 1549 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31709 fis, clone NT2RI2006345.
ACCESSION AK056271
VERSION AK056271.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1549)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1549
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2006345"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012885"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 278..745
/codon_start=1
/protein_id="BAB71132.1"
/translation="MPVSYSSVTTRYYHRAGAEEGKDRIIFVTKEDHETPSSAELVAD
DPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVD
QFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS"
BASE COUNT 406 a 345 c 400 g 398 t
ORIGIN
1 aggcgcgcgg cgggcaggcc tgggaggggg cgggtccgcg cgggcttcgg aggagcccgc
61 cctgccagag gagagggagg tcacggcgta aaggtgcagc tgccgccacc gccgcttctg
121 caaggtctca gggacgggct gcagccatgt cctattgccg gcaggaaggc tttgcccagg
181 atggttcctt ctggaatgtc tattcccctc cttccctccc tgaagctctt tgtcagggac
241 aaaggtagga tccctcccct tccgtcttct ccactggatg cctgtgtcgt attcatctgt
301 gaccaccagg tactaccaca gagctggagc tgaggaaggg aaggatcgaa tcatatttgt
361 aaccaaagaa gatcatgaaa ctccaagcag tgcagaattg gtggctgatg accccaacga
421 tccatacgag gagcatggat tgatactgcc aaatggaaac attaactgga actgcccatg
481 ccttggggga atggccagcg gtccctgtgg agaacagttt aagtcagcct tttcctgctt
541 ccactatagc acggaggaga tcaaggggtc agactgtgta gaccagttcc gggccatgca
601 ggaatgcatg cagaaatacc cagacctcta tccccaagag gatgaggatg aggaagagga
661 aagagagaag aagccagcag aacaagcaga agaaacagct cccattgagg ccactgcaac
721 caaagaagag gagggatcaa gttaatgaag gccacaaggc actgggcacc agtccttttg
781 gagtggacct tttgcaaaag gccttgtcat caccttccaa gaaagtttcc ttctgttgtc
841 ctgtgcatta taatatacaa aataacttat tttgatgatc agaggtcttg aggtcttgac
901 atcttgacat atacactgaa aaaaatgggg gttgtatgta tgtgtgtcct acccaaacct
961 gtggccgcca cttttgaatt ctcagattgc cctgaatttt gccactttta aataatgtgc
1021 tgaataagct cagcaactaa aaaccattac ccaagaacgt ttcttgtgag tgagctgatt
1081 tattctgatt cattatattc cttttggtag attttatacc ccttggggaa ataatacaac
1141 aaaaacatct cttaaaaatg ctgggatggg gccatatcta ctagcagagg ccagatggtc
1201 agatatgatt tctgcaaacc catcttgacc ttgagtatgt gaaggggtac tgtactttat
1261 tcctgataca ttttggtttc catgtaggtg ttgagctcct ggttttctgt gtttggatga
1321 tgaagatttg gacccttcca ttcataatcc ctttctaagt gaagggagag gctggcttgg
1381 ctgttccttg ttattccgaa agccctggtt tggggcccat gttcacactg gctctcagtc
1441 tagtcaggtg caatgttctt gagaggtggg gacctaatta ttaccagagt agcagcaaga
1501 gaggaaacgt tgtgaattaa gtattcaatt aaaaggaaac atgatttct
//