LOCUS AK056260 1984 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31698 fis, clone NT2RI2005966.
ACCESSION AK056260
VERSION AK056260.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1984)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1984
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2005966"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012874"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 399 a 537 c 539 g 509 t
ORIGIN
1 ggcagtatgt agccccgaag actgttgccc aagtggtaga atgttagcac actaccagcc
61 taggtaaaaa atacaaaaag taactgggca tggcggcgcc catctatagt cccggctgca
121 tgggaggctg aggtgggaag ataagtcact tgagcccgcc aggaggcgga ggttgtagtg
181 agctgggatc gcgccactgc actccagcct gggtgaccga gtgatactct gtctcaaaga
241 aaaaaaatta taattttagc acagtaacca gccatgatgg gagataccct gggtaaggca
301 tgtagaaagg gttgagggac cttcccagtc ccctagcccc gcctcccatc ctcccatctt
361 tttctttttt ctttttttta gagaatcacc cagcctggag cgaagtggtg caatcataac
421 tcactgtatc cttaaactcc cgggcttaag cgatcctcct gcctcagcct tctgagtaac
481 tgggacttca ggtacctgtc accatgcctg gctaattaaa tttttttttc tttttttttt
541 tgagatggag tcttgctctg tcgcccaggc tggagtgcag tggcgcgatc tcggctcact
601 gcgacctccg cctcctgggt tcaggccatt ctcccgcctc agcctccaga gtagctggga
661 ctgcaggcgc ctgccaccgc gcctggctaa tttttttgca cttttagtag agacggggtt
721 tcactgtgtt ggccaggatg gtctcgatct cctgaccttg tgatccgccc gcctcggcct
781 cccggggtgc tgggattgca ggcgtgagcc accgcgccca gccaaattaa attttttgtg
841 gagatgaggt catgctgtta tgttgcccag gttggcctca tgagagcttg ccttggcctc
901 ccgaagtgct gggattgcag atgtgagaca ctgcacccaa accccaccac tttttttttc
961 ctttttcttt ttttgagaca gtcttgctcc gttgcccagg ctggagtgta gtggcatgat
1021 ctcggctcac tgcagcctcc gcctcccggg ttcgggcaat tctcctgcct cggcctcccg
1081 agtagctggg attgcagagg cctgccacca cacccgactg attttcgtat ttttagtaga
1141 gacggggttt ctccatgttg gccaggctgt tcttgaactc ctgacctcaa gtgctccacc
1201 tgcgttggct tcccagggtg ctgggataca ggagtgagcc actgcgcctg gctgatccca
1261 gcacttttca aatgatgccg ctcaaagccg tgacttggcc tactttgaac agcaaacttg
1321 ttgctgctgt tgtcaacctg aaggcctctc aaatgccagc ttcaagcagg gtgtgaattg
1381 gccagtgtca gatctcagga gtcctgtgtt gagagtgtgg ctttcagctg cggggagctg
1441 cacttggtgg gggaagccag gcaggtcacc ctcacagcca gataatgtgg aggtcagaac
1501 ccaaggaagg gagtgagacc tccactccca gtgggggacc tggccaccca tccttgggga
1561 cctgagaaag cgtacttcac cttggggtga aggctgggtg gggccagagg gaccagtgcc
1621 ctcctcagtg cttaggggca gagccacctg cagcaatggt atctgcatat tagcccctct
1681 ccaccttctt tctcccgctg aatcatttcc ctcaaagccc aagagctgtc actgcttctt
1741 tctccctggg aagaatgcgt ggactctgcc tggtgataga ctgaagccag aacagtgcca
1801 caccctcgcc ttaattcctt gctaggtgtt ctcagattta tgagacttct tagtcaaata
1861 tgagggaggt tggatgtggt ggcttgtgcc tgtaatccca gcattttggg aggccgaggt
1921 gggaggatcc cttgaggcca ggagtttgag acaagcctgg gcaacaaagc aagaccctat
1981 ctct
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