LOCUS AK056255 2043 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31693 fis, clone NT2RI2005710, highly similar
to BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE, CYTOSOLIC (EC
2.6.1.42).
ACCESSION AK056255
VERSION AK056255.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2043)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2043
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2005710"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012869"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 528..1688
/codon_start=1
/protein_id="BAB71129.1"
/translation="MKDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDP
NNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFR
GVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASL
YIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGT
GDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEEL
ATPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLSTALEGNRVREMFGSGTA
CVVCPVSDILYKGETIHIPTMENGPKLASRILSKLTDIQYGREESDWTIVLS"
BASE COUNT 527 a 461 c 524 g 531 t
ORIGIN
1 agtagggagg tgggcaggag ccagtgatga cggaatggca atcacatttg acctctgatc
61 tgtttatttc ctcctccttg acgtctccat ataaatgtta cacgggcatc cccacactcg
121 gatacgcacc cacagtggct gattcggggg taaccgtgtc atttgcttgc aacactggca
181 cctctgccct gcaccccggg agtgagcagt gagtgaggct cgggtctggg cgctggctcc
241 gaatcttcgg gctgggagag actccaccat ctgggggcgg cctgggggag cagccttagt
301 gtcttcctgc tgatgcaatc cgctaggtcg cgagtctccg ccgcgagagg gccggtctgc
361 aatccagccc gccacgtgta ctcgccgccg cctcgggcac tgccccaggt cttgctgcag
421 ccgggaccgc gctctgcagc cgcagacccg gtccacacgg ccaggggcta cgacccttgg
481 gatctgccct ccgctcagct cgagcttccc tcgtggccga cggaacaatg aaggattgca
541 gtaacggatg ctccgcagag tgtaccggag aaggaggatc aaaagaggtg gtggggactt
601 ttaaggctaa agacctaata gtcacaccag ctaccatttt aaaggaaaaa ccagacccca
661 ataatctggt ttttggaact gtgttcacgg atcatatgct gacggtggag tggtcctcag
721 agtttggatg ggagaaacct catatcaagc ctcttcagaa cctgtcattg caccctggct
781 catcagcttt gcactatgca gtggaattat ttgaaggatt gaaggcattt cgaggagtag
841 ataataaaat tcgactgttt cagccaaacc tcaacatgga tagaatgtat cgctctgctg
901 tgagggcaac tctgccggta tttgacaaag aagagctctt agagtgtatt caacagcttg
961 tgaaattgga tcaagaatgg gtcccatatt caacatctgc tagtctgtat attcgtccta
1021 cattcattgg aactgagcct tctcttggag tcaagaagcc taccaaagcc ctgctctttg
1081 tactcttgag cccagtggga ccttattttt caagtggaac ctttaatcca gtgtccctgt
1141 gggccaatcc caagtatgta agagcctgga aaggtggaac tggggactgc aagatgggag
1201 ggaattacgg ctcatctctt tttgcccaat gtgaagcagt agataatggg tgtcagcagg
1261 tcctgtggct ctatggagag gaccatcaga tcactgaagt gggaactatg aatctttttc
1321 tttactggat aaatgaagat ggagaagaag aactggcaac tcctccacta gatggcatca
1381 ttcttccagg agtgacaagg cggtgcattc tggacctggc acatcagtgg ggtgaattta
1441 aggtgtcaga gagatacctc accatggatg acttgtcaac agccctggag gggaacagag
1501 tgagagagat gtttggctct ggtacagcct gtgttgtttg cccagtttct gatatactgt
1561 acaaaggcga gacaatacac attccaacta tggagaatgg tcctaagctg gcaagccgca
1621 tcttgagcaa attaactgat atccagtatg gaagagaaga gagcgactgg acaattgtgc
1681 tatcctgaat ggaaaataga ggatacaatg gaaaatagag gataccaact gtatgctact
1741 gggacagact gttgcatttg aattgtgata gatttctttg gctacctgtg cataatgtag
1801 tttgtagtat caatgtgtta caagagtgat tgtttcttca tgccagagaa aatgaattgc
1861 aatcatcaaa tggtgtttca taacttggta gtagtaactt accttacctt acctagaaaa
1921 atattaatgt aagccatata acatgggatt ttcctcaatg attttagtgc ctccttttgt
1981 acttcactca gatactaaat agtagtttat tctttaatat aagttacatt ctgctcctca
2041 acc
//