LOCUS AK056254 2617 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31692 fis, clone NT2RI2005647, highly similar
to KERATIN, TYPE II CYTOSKELETAL 4.
ACCESSION AK056254
VERSION AK056254.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2617)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2617
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2005647"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012868"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 581 a 692 c 772 g 572 t
ORIGIN
1 gacttgctcc ggtttgcaga gctaggaggt ggcaggctgt gcgctcaaac tcaggctgtc
61 taactccaca ttctgtgggg tgagaggatg ggtgatgggg tgtcttttct ggaggaggga
121 ggtgctgtga gcctagcgag atggaggtac agtgggtgtg ggcctggagc gctgggccca
181 ggcaggggct tctgattagg aagccctggg gcaccagttc aggttctccc agagagtagt
241 gtgatgggat ccagtaacct gtgccctcca gatgacttct gtaggtgtgt ttagtgacat
301 gctcaacggg tgcgggaagg atgggcttgt gccaagggcc aagcccagag atgtttcaga
361 tttttccctt tatgcccctg caaccaagcc ctgctgctcc aggacatata agagacgaag
421 gctgagggct ccagcactca ccggcctggg ccctgtcact tctctgatag ctcccagctc
481 gctctctgca gccatgattg ccagacagca gtgtgtccga ggcgggcccc ggggcttcag
541 ctgtggctcg gccattgtag gcggtggcaa gagaggtgcc ttcagctcag tctccatgtc
601 tggaggtgct ggccgatgct cttctggggg atttggcagc agaagcctct acaacctcag
661 ggggaacaaa agcatctcca tgagtgtggc tgggtcgcga caaggtgcct gctttggggg
721 tgctggaggc tttggcactg gtggctttgg tgccggcggc ttcggagctg gtttcggcac
781 tggtggcttt ggtggtggat ttgggggctc cttcagtggt aagggtggcc ctggcttccc
841 cgtctgcccc gctgggggaa ttcaggaggt caccatcaac cagagcttgc tcacccccct
901 ccacgtggag attgaccctg agatccagaa agtccggacg gaagagcgcg aacagatcaa
961 gctcctcaac aacaagtttg cctccttcat cgacaaggtg cagttcttag agcaacagaa
1021 taaggtcctg gagaccaaat ggaacctgct ccagcagcag acgaccacca cctccagcaa
1081 aaaccttgag cccctctttg agacctacct cagtgtcctg aggaagcagc tagatacctt
1141 gggcaatgac aaagggcgcc tgcagtctga gctgaagacc atgcaggaca gcgtggagga
1201 cttcaagact aagtatgaag aggagatcaa caaacgcaca gcagccgaga atgactttgt
1261 ggtcctaaag aaggacgtgg atgctgccta cctgaacaag gtggagttgg aggccaaggt
1321 ggacagtctt aatgacgaga tcaacttcct gaaggtcctc tatgatgcgg agctgtccca
1381 gatgcagacc catgtcagcg acacgtccgt ggtcctttcc atggacaaca accgcaacct
1441 ggacctggac agcattattg ccgaggtccg tgcccagtac gaggagattg cccagaggag
1501 caaggctgag gctgaagccc tgtaccagac caaggtccag cagctccaga tctcggttga
1561 ccaacatggt gacaacctga agaacaccaa gagtgaaatt gcagagctca acaggatgat
1621 ccagaggctg cgggcagaga tcgagaacat caagaagcag tgccagactc ttcaggtatc
1681 cgtggctgat gcagagcagc gaggtgagaa tgcccttaaa gatgcccaca gcaagcgcgt
1741 agagctggag gctgccctgc agcaggccag ggaggagctg gcacgaatgc tgcgtgagta
1801 ccaggagctc atgagtgtga agctggcctt ggacatcgag atcgccacct accgcaaact
1861 gctggagggc gaggagtaca gaatgtctgg agaatgccag agtgccgtga gcatctctgt
1921 ggtcagcggt agcaccagca ctggaggcat cagcggagga ttaggaagtg gctccgggtt
1981 tggcctgagt agtggctttg gctccggctc tggaagtggc tttgggtttg gtggcagtgt
2041 ctctggcagt tccagcagca agatcatctc taccaccacc ctgaacaaga gacgatagag
2101 gagacgaggt ccctgcagct cactgtgtcc agctgggccc agcactggtg tctctgtgct
2161 tccttcactt cacctccatc ctctgtctct ggggctcatc ttactagtat cccctccact
2221 atcccatggg ctctctctgc cccaggatga tcttctgtgc cgggacaggg actctgcctc
2281 ttggagtttg gtagctactt cttgatttgg gcctggtgac ccacctggaa tgggaaggat
2341 gtcagctgac ctctcacctc ccatgggcag agaagaaaat gaccaggagt gtcatctcca
2401 gaattattgg ggtcacatat gtcccttccc agtccaatgc catctcccac tagatcctgt
2461 attatccatc tacatcagaa ccaaactact tctccaacac ccggcagcac ttggccctgc
2521 aagcttagga tgagaaccac ttagtgtccc attctactcc tctcattccc tcttatccat
2581 ctgcaggtga atcttcaata aaatgctttt gtcattc
//