LOCUS AK056249 1893 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31687 fis, clone NT2RI2005473.
ACCESSION AK056249
VERSION AK056249.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1893)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ishibashi,T., Kanehori,K., Yosida,M., Watanabe,S., Ishida,S.,
Ono,Y., Hotuta,T., Hiraoka,S., Murakawa,K., Takiguchi,S.,
Kusano,J., Watanabe,M., Fujimori,K., Tanai,H., Ishida,M.,
Yamashita,H., Chiba,Y., Sugiyama,T., Irie,R., Otsuki,T., Sato,H.,
Wakamatsu,A., Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y.,
Saito,K., Nishikawa,T., Kimura,K., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1893
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2005473"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012863"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 573 a 398 c 411 g 511 t
ORIGIN
1 aaagggacca gcgcgtgccc tactggcggt ggagaggctg ggcgggcccc ataggatgcc
61 cgctttggag ccacaggacc tcaaaatacg ctccttacaa cgtgttttat agagcgaagt
121 ctcgctatgc cacccaggct ggagtgcagt ggcgccatcg tagctcaccg cagcctcgaa
181 cacctgggct caagcgatcc tcccgcctca acctcccgag tagctgggac cacaggtgcg
241 cgccaccaag ccgggctcta acagttttta accagacctg ttgatggttt ctggggcgag
301 cactagctgg ggatttttat atcagctgga ttagtttttt ccccctaaac gagtctacaa
361 aatcccggac atttccaggg tcctgagacg gttccctacc ttccttctgc gcagagattg
421 cctgttgggg atgaatcgct tagcgagacc cagccatagc tggctacgct gagttccggc
481 ccgggaccgc gcggagaggc aagatctatt cccgaccctc tcatctggca gacagaaccc
541 aggcttcagg aagagaagcg gctttcgcag tttcacgcta ggacgggctg agctgggaat
601 cgatcccaaa ttccacagtc ccagtcctct cctgggtcct gctctcctga ctgcgtctca
661 ggcccagagg tgatggggca cttagggtta gggaatggca gacctggaga aaagggttcc
721 gctactaccc tcggctttgc caaacttaac attttgaaat tccattccac aaatgtgtac
781 tgcgtctctg ggaacaagac agcgtaatga gcatcgtgag aaatctaaga cgaatcccta
841 aaccgtcgct gtctttaaag agattacaac catgctataa ttacatacag agctgtatct
901 ctaataagga cccaaccacg gttttatctg gctagttttt ttgtttgttt tgagctctgg
961 ttatttccca tagccaggaa aatcaaaata ttaaaaacaa gggatagggg aaggccagaa
1021 gaaaaaaaca acgctgcaac tacctaaagc actgtgttaa ttaaccgcat ttgctgagtg
1081 ccttctgtag gtaaaagcac catactatgg agggaaacag aaaataagta ttaaaagata
1141 tggtttccac ttccagtagt tttcaatgct ctttgctgtt tcaggggtat aaactaaata
1201 agtaaagcaa gatggaaaaa aattagcaaa tgacaacttt aaataataag cttttaacta
1261 gaaaattgat aaaccaagag taaatattta aggattataa atatgcatac taaacaccct
1321 ttcatgagat cagatggtgg gtttcaattt tacataaaaa ttatggtaac tggaaaccgg
1381 gcggattaag tagtgtagat gaacagacaa gatattgcat atgtgtagag gaaagataaa
1441 tatttgtatt aggtgccatt caaatatggt agtcttttca ctacatagaa ctatgccttg
1501 aaagcaatag atactacact gtataactta attattaact taagctctga ttaagcaaac
1561 tcagtaaaac tttcaattgt ccctcagact tgaaatgtat tactgcatca gggataaagg
1621 aaaagtcaaa attatctctt attttggtag atgaaattga aaaagttgca gtgtgtgtgt
1681 acatggtgca tccccccaag atctatccat aagaatggct tgctttttgc ttatttatat
1741 aaaacccact atgttaaaac aatgattttg tttaataatt tacaaattta tcaatgattt
1801 atacatctta attttcagta atatgcagta tttgttgcca aatggtgtaa tacaattatg
1861 aatgtctgaa gataataaaa ccaagatgag tag
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