LOCUS AK056221 2174 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31659 fis, clone NT2RI2004398.
ACCESSION AK056221
VERSION AK056221.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2174)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2174
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2004398"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012835"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 76..549
/codon_start=1
/protein_id="BAB71123.1"
/translation="MEGCAVRRGSCPLLPGPSAWRASPAGWAGRAKLRSWCRASGLPN
RPYTLTGGRHGSVSLLRHPGTTTFVQQRSLHQSWEKRIVFSACPVSRSWCPERNFSGS
IPAVTPPKLPGHSKSEGPPGKVRKRTTIRSQPLFVTRTRGFGSAVGWLPLGSPVL"
BASE COUNT 689 a 424 c 441 g 620 t
ORIGIN
1 gcttcgactt ccgggtgaga ccgtgaaggt gtggcgcgac gttggagacc ggggcagcgc
61 catgtaccac agtggatgga aggatgcgcg gtgcggcggg gcagctgtcc tcttctcccc
121 ggacccagcg cctggagagc cagccctgca gggtgggctg ggcgagccaa actgcgttcc
181 tggtgcaggg cttcgggtct ccctaacaga ccttatacgc tgaccggcgg ccgccatggc
241 agtgtctctt tgctcagaca tccagggacg accacattcg tccaacagcg gtcgctccac
301 caatcctggg agaagcgaat cgttttctcc gcgtgccctg tcagccgctc atggtgccca
361 gagaggaatt ttagtggcag cattccggct gtcacgccac cgaaattgcc aggccactcc
421 aagtcagaag gaccaccagg aaaagtcagg aagagaacca ccatcaggtc ccagcctctt
481 tttgtgacaa ggactagagg gtttgggtct gcagtgggct ggctcccgct gggctcacct
541 gtcctataga gcaggctgcc tcaaggaaga agctcacagg ggacatctga catctgaaac
601 agcaagtgta aaatgcttta aataagccaa aactaaacca atcgttatgt taactaaaaa
661 tacatgaatg ctaccgaaga tagtcgaata ttagaggaag agattaaact ctgattttga
721 gcactttgtg ctttattcgg aaaatgaata agatgcagtt ctgaaaacct gaaaatataa
781 agaataggat atgatacgca cactagatga aaacaaaact ttcagaccat attttcttat
841 ttaaagctct aataagtgag atagcagagt aaactattca ctcaaataat tagtaactga
901 tataagggta tgtatcaata acaggtatta agacaaaaac tttatgagct tttccatttt
961 aacaagggat gatgattcta gacttctgcc acaacattat ggtcgacaaa acattcttaa
1021 ttcattttac atagatctga ctctagattc tgaaacagca agtcataatc ttgtatctct
1081 aaagatagaa tacagtgcga tttggaaaga caccacaaaa actcatgatc caaaaaagat
1141 taaggctgtt attaccagga ggaagagaaa gaggacaaaa tcaaactgga tagatataat
1201 agagttgttt gttagaattg tcttcccagg agatggaaaa agacgccatc acaataggat
1261 tcatgcttta gttttcagct atagaaatgt aataactttg ccaaagtaat tacactacat
1321 agagtggcaa tcgttctatt aaaagtaaga cccagtaatg ttaggatttg ttctggacaa
1381 tgaaggggat gaggtttaat cttatgactt gaatgccagg tctgaaatct tttcttcaag
1441 tgatattttt atgaaactac ttaggctgtg tttcacactg ggtatgattt ctaacctctt
1501 ataacctgta cagtaataga ttatgaagga tgaattattt gaaagctatt ttgtattatt
1561 taggagagga gctcttcaat tctgaattgt ctgccactta caagcagaag aaagtttttt
1621 tctttcatta ttttagcaac tatcaataaa acagtattag aaatgcctac tcttcagaga
1681 tgtaatggga ataaattgta aagtactttt gatattctaa gatgaaagat ggtattacac
1741 agtcaataat tcaatgcttc ccacattatg aattttatca aaattatgta gagagctatt
1801 agttcattcc agcaacaaaa caggcttata taaaaacaaa aacctcctgg gtcatgccat
1861 cctacaccaa attcctgtta aacagaggca tgatttattc cagtgacctt gctatttctt
1921 cataactgta atatgtctaa tgttagacat tttaaattaa cattctatta gataaactga
1981 gaatttaggt ctctcgcacc acccaggcac atactctatc tttccccaca aatatccaaa
2041 tatggtaata taattggatt aatacgttca tatgactata taattgttca cagctgagcc
2101 acttaggtgc tataattata atttctttga tacacaaatt ttgttttatt gtgattaaaa
2161 ttgcctgatt tctt
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