LOCUS AK056206 2661 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31644 fis, clone NT2RI2003751.
ACCESSION AK056206
VERSION AK056206.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2661)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2661
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003751"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012820"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 591..1589
/codon_start=1
/protein_id="BAB71120.1"
/translation="MPEGPLVRKFHHLVSPFVGQQVVKTGGSSKKLQPASLQSLWLQD
TQVHGKKLFLRFDLDEEMGPPGSSPTPEPPQKEVQKEGAADPKQVGEPSGQKTLDGSS
RSAELVPQGEDDSEYLERDAPAGDAGRWLRVSFGLFGSVWVNDFSRAKKANKRGDWRD
PSPRLVLHFGGGGFLAFYNCQLSWSSSPVVTPTCDILSEKFHRGQALEALGQAQPVCY
TLLDQRYFSGLGNIIKNEALYRAGIHPLSLGSVLSASRREVLVDHVVEFSTAWLQGKF
QGRPQHTQVYQKEQCPAGHQVMKEAFGPEDGLQRLTWWCPQCQPQLSEEPEQCQFS"
BASE COUNT 559 a 667 c 801 g 634 t
ORIGIN
1 gtagcggtgc tgggtcgggc cgacgtgcca cccacccgga gccggtgagt gcagccgccc
61 gccctccggt agatctgcgg cctggcggag aagtcgggag gggacaggaa gggagggcgg
121 gccccgggcc ctcctccgtc tcagccgcct gcggaggtgc tgcccacgcc tggaggcccc
181 cactgaccct cagacccgcg tctgcgcccc tctccccgca ccccgaggca gagttgggaa
241 agcagtggtc ttagaccccc cacctcgggc actcggaaga gaacggcgga gacaacccct
301 cctcttccct ggctggcgca gcgccagcct cgagctcctc ggtagccccc gggcagggag
361 ggcccgaggg tgggcgcggc atcttcagcg actcttcgaa gtcccttccg cgtctcatct
421 ttcaaggctg ttgcagaggc ggcttgcttc ccacctgtcc atctccataa aaatccctaa
481 acgaaacatg cccacgtgtc cggagatttt caggacttgg tgcatttcag atgaaggctt
541 ttccagaagc ttccccgtag aagaggatca ggcatccaac tggttaaggg atgccagaag
601 ggccgttggt gaggaaattt caccatttgg tctccccctt tgtgggtcag caggtggtca
661 agacaggggg cagcagtaag aagctacagc ccgccagcct gcagtctctg tggctccagg
721 acacccaggt ccatggaaag aaattattcc ttagatttga tctagatgaa gaaatggggc
781 cccctggcag cagcccaaca ccagagcctc cacaaaaaga agtgcagaag gaaggggctg
841 cggacccaaa gcaggtcggg gagcccagcg ggcagaagac ccttgatgga tcctcacggt
901 ctgcagagct cgtcccccag ggcgaggatg attctgagta tttggagaga gacgcccctg
961 caggagatgc tgggaggtgg ctgcgtgtca gctttggttt gtttggcagc gtttgggtga
1021 acgatttctc cagagccaag aaagccaaca agagggggga ctggagggac ccttccccga
1081 ggttggtcct gcactttggt ggtggtggct tcctggcatt ttataattgt cagttgtctt
1141 ggagctcttc cccggtggtc acacccacct gtgacatcct gtctgagaag ttccatcgag
1201 gacaagcctt agaagctcta ggccaggctc agcctgtctg ctatacactg ctggaccaga
1261 gatacttctc agggctaggg aacatcatta agaatgaagc cttgtacaga gctgggatcc
1321 atcccctttc tctcggttca gtcctgagtg cctcgcgtcg ggaggtcctg gtggatcacg
1381 tggtggagtt cagtacagcc tggctgcagg gcaagttcca aggcagaccg cagcacacac
1441 aggtctacca gaaagaacag tgccctgctg gccaccaggt catgaaggag gcgtttgggc
1501 ccgaagatgg gttacagagg ctcacctggt ggtgcccgca gtgccagccc cagttgtcag
1561 aggagccaga gcagtgccag ttctcctaag gagctggtgg tgctcctcat ggaaccttgc
1621 cgttggggaa cctgacgtct aagtgtccag aaaggaggat gtgggcaggg acggggtaca
1681 gaggatagtg tgggtcagag gtgccagtag tataatattc atctccctgg agttatgttg
1741 aaggcagagt tttcataggg ttagattttt tttatccttt tctagttcag ttaattcatc
1801 ctgttgaatt gcaccatcgt gaaagatggg aaaaatcgtg atgatgggta aggggaaaac
1861 ttcccggaag gcaatggggc aaggaaaaag aaagcctatg ggaaatggct gtgctcccaa
1921 catagctttg cagatgatgt gggttttttt tttttttttt ggttgtttgt tttgagagag
1981 agtcttgctc tgtctccctg gctagggtgt ggtggtgtga tcttggctca cggcagcctt
2041 gccctccctg gctcaagcag tcttcatctc agcctccaga gtagctggga ctacaggcat
2101 gtgatatgat gctcggctga tttttgttta ctttttagag agatggggtc ttgccatatt
2161 tgccaggctg gtttgaactc ctacaactca agcattcctc ccaccttggt ctcccaaaat
2221 gttgggacca cgggtgtgag ccaccgcgcc cagctagctc ctgtgttttg tttttgtttt
2281 gtaactttgg ttgatgttaa ggccctccat tttggaaagc aggaaaacag gatttttttt
2341 tttttatctt gttccctgga ggatccaggg atgaggatag agtggcctga gagcagtgct
2401 tggattcagc ctcctgctgg gtccttctgc tggatacagg caccaagagg cgggggtgga
2461 gcagggagct gcgccttcct ggggtgcccg gtggtgtgta gagaaaagct gcttgtttac
2521 tccttaagtc aatgtattgg tgactgttga tttgttgaac aattcaggaa tcaagggctg
2581 tggagaaact ccctcatgtt gttggcaaca ggtgaatgaa cctagagcgg tgacatgaaa
2641 ataaagctca ctgttactcg c
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