LOCUS AK056203 3045 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31641 fis, clone NT2RI2003695, weakly similar
to TRICHOHYALIN.
ACCESSION AK056203
VERSION AK056203.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3045)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..3045
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003695"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012817"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 355..2445
/codon_start=1
/protein_id="BAB71119.1"
/translation="MPLFFRKRKPSEEARKRLEYQMCLAKEAGADDILDISKCELSEI
PFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQ
LTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELPDTVGELRSLRTLNISGN
EIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQY
LLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLEL
GQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQN
ISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDFASAMQQM
LTESCKNRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQES
AMQKAAFEALQVKKDLMHRQIRSQEMISEQRWALSSLLQQLLKEKQQREEELREILTE
LEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHH
RLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAARIQPELKPPMGEVVTPTA
PQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLC
RQDIAQRLRIYHSS"
BASE COUNT 681 a 913 c 900 g 551 t
ORIGIN
1 ttgtttgttg tgggcaggcg cctgaggctg acggctggca agcagggcac cgcggtgcgc
61 tgaagctaga gattccgaaa gggggttcgg gtagttcgct ccggagaagt ctgagaaggg
121 tggctccggt gatcccagcc ctagctgttt cccatattcc tttatccacc tgcctttgaa
181 ggagaccaga ctccggctcc gcccggcccg tgccgggtgg tctgcggccc cgagtccgtg
241 accaagccct ccgagtgatc ggtctgccct cgggtgcacc cgcgggtccc aacgtggggg
301 atccctccat ccgcaaagcc agggtcctaa agatcgctct gggaaaaggg aaggatgccg
361 ctcttcttcc ggaagcggaa acccagtgag gaggctcgga aacgcctgga gtaccagatg
421 tgtttggcaa aagaagctgg ggcagatgac attctcgaca tctctaaatg tgagctctca
481 gagattccat ttggagcttt tgcaacatgc aaagttctgc agaagaaggt gctgatcgtc
541 cacacgaatc acctcacttc cctgcttccc aaatcctgca gcctcctgag tctggcaacc
601 atcaaggttc tagatctcca cgataatcag ctgacagccc ttcctgacga tctggggcag
661 ctgactgccc tccaggtctt aaacgtggaa aggaatcaac tgatgcagct cccacgttcc
721 attgggaacc tgacccagct ccagactctc aatgttaaag acaacaagct gaaggagctt
781 ccagacaccg tgggggagct tcgaagcctg cgtaccctca acatcagtgg aaacgagatc
841 cagagattgc cgcagatgct ggctcacgtt cgaaccctgg agatgctgag ccttgacgcc
901 tcggccatgg tctacccgcc gcgggaggtg tgtggtgccg gcactgcggc catcttgcag
961 ttcctctgca aagagtcagg gctggaatac tacccccctt ctcagtactt gctgccaatt
1021 ctggagcaag atggaatcga gaactctcgg gacagccctg atgggcccac ggacagattc
1081 tcaagggagg agttagagtg gcagaacagg ttctcagact atgagaagag gaaggaacag
1141 aagatgctgg agaaactcga gtttgaacgg cgcctggaac tggggcagcg ggagcacacc
1201 cagctccttc agcagagcag cagccagaag gatgagatcc ttcagacggt caaggaggag
1261 cagtcccggc tggagcaggg cctgagtgag caccagcgcc acctcaacgc agagcggcag
1321 cggctgcagg agcagctgaa gcagacggaa cagaacattt ccagccggat ccagaagctg
1381 ctgcaggaca atcagagaca aaagaaaagc tccgagattt tgaaatcgct ggaaaatgaa
1441 agaataagaa tggaacagtt gatgtccata acccaggagg agactgagag cctgcggcga
1501 cgtgactttg cctccgccat gcagcagatg ctgactgaga gctgtaagaa ccggctcatc
1561 cagatggcct acgaatctca gaggcagaac ttggtccagc aggcctgttc cagcatggcc
1621 gaaatggatg aacgattcca gcagattctg tcgtggcagc aaatggatca gaacaaagcc
1681 atcagccaga tcctgcagga gagcgcgatg cagaaggctg cgttcgaggc actccaggtg
1741 aagaaagacc tgatgcatcg gcagatcagg agccaggaga tgatctcgga gcagcgctgg
1801 gccctcagct ccctgctcca gcagctgctc aaagagaagc agcagcgaga ggaagagctc
1861 cgggaaatcc tgacggagtt agaagccaaa agtgaaacca ggcaggaaaa ttactggctg
1921 attcagtatc aacggctttt gaaccagaag cccttgtcct tgaagctgca agaagagggg
1981 atggagcgcc agctggtggc cctcctggag gagctgtcgg ctgagcacta cctgcccatc
2041 tttgcgcacc accgcctctc actggacctg ctgagccaaa tgagcccagg ggacctggcc
2101 aaggtgggcg tctcagaagc tggcctgcag cacgagatcc tccggagagt ccaggaactg
2161 ctggatgcag ccaggatcca gccagagctg aaaccaccaa tgggtgaggt cgtcacccct
2221 acggcccccc aggagcctcc tgagtctgtg aggccatccg ctccccctgc agagctggag
2281 gtgcaggcct cagagtgtgt cgtgtgcctg gaacgggagg cccagatgat cttcctcaac
2341 tgtggccacg tctgctgctg ccagcagtgc tgccagccac tgcgcacctg cccgctgtgc
2401 cgccaggaca tcgcccagcg cctccgcatc taccacagca gctgagtgct gcccgcccac
2461 ctgggcctgg tcctagccct gcctcggcca ctgtgagccc cgggctcctg ctcagccttg
2521 tgccagccag actcgtatga ggctcccccc tgccctgggc cccttcccca ctgcccagga
2581 gcccccatcc taagctccaa gcatgtctgg gccaggcaga ggtgctcctc atccatgaca
2641 ccaccagtct gaatggtcct gggggctggg gctggagagg ccgctgcacc accacccgag
2701 cctgggagcc agcgtcccag cctaatcacg gatctgctgc ctcccagctg tcttgactga
2761 aggccaccgc ccctgcagga gcttgggtcc tcatctgggg gccatgcaca ggcccgtccc
2821 accctgcatg tgggaaggga gcaggagggc ctggctgggt gaggggaggc cttcctggga
2881 aggcgtgtgg tgcaggcctg tgctcacagt ggcaccagca accctgggtc tccctctctg
2941 ctgctcccca gaaccccggg gccctcctgc tctccacaac tgtccctcct taccccatgt
3001 agctcgatcc gaagcaggag tgtcaataaa cctgtcttca gtgcg
//