LOCUS AK056200 2035 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31638 fis, clone NT2RI2003556.
ACCESSION AK056200
VERSION AK056200.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2035)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2035
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003556"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012814"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 27..1883
/codon_start=1
/protein_id="BAB71118.1"
/translation="MAAVAAGGLVGKGRDISLAALQRHDPYINRIVDVASQVALYTFG
HRANEWEKTDVEGTLFVYTRSASPKHGFTIMNRLSMENRTEPITKDLDFQLQDPFLLY
RNARLSIYGIWFYDKEECQRIAELMKNLTQYEQLKAHQGTGAGISPVILNSGEGKEVD
ILRMLIKAKDEYTKCKTCSEPKKITSSSAIYDNPNLIKPIPVKPSENQQQRIPQPNQT
LDPEPQHLSLTALFGKQDKATCQETVEPPQTLHQQQQQQQQQQQEKLPIRQGVVRSLS
YEEPRRHSPPIEKQLCPAIQKLMVRSADLHPLSELPENRPCENGSTHSAGEFFTGPVQ
PGSPHNIGTSRGVQNASRTQNLFEKLQSTPGAANKCDPSTPAPASSAALNRSRAPTSV
TPVAPGKGLAQPPQAYFNGSLPPQTVGHQAHGRERSTLPRQTLPISGSQTGSSGVISP
QELLKKLQIVQQEQQLHASNRPALAAKFPVLAQSSGTGKPLESWINKTPNTEQQTPLF
QVISPQRIPATAAPSLLMSPMVFAQPTSVPPKERESGLLPVGGQEPPAAATSLLLPIQ
SPEPSVITSSPLTKLQLQEALLYLIQNDDNFLNIIYEAYLFSMTQAAMKKTM"
BASE COUNT 562 a 576 c 458 g 439 t
ORIGIN
1 gtgcctatgt ctgggaggga gggaagatgg cagccgtggc ggcaggcggc ctggtgggaa
61 aggggcgcga catcagccta gcggccctgc agcgccacga cccctatatc aaccgcatcg
121 tggacgtggc cagccaggtg gctctgtaca ccttcggcca tcgggccaac gagtgggaga
181 aaactgatgt ggaaggaacc ttatttgttt atacaaggtc tgcttctcca aagcatggat
241 tcaccattat gaataggctg agcatggaaa ataggacaga acctattact aaagacttgg
301 atttccaact ccaggaccct ttccttctct acagaaatgc cagattgtcc atctatggaa
361 tttggtttta tgataaggaa gaatgccaaa gaattgcaga gcttatgaaa aacctaactc
421 agtatgaaca gttgaaagcc catcagggaa ctggagcagg aatttcccca gtgatcctca
481 attcaggaga gggcaaagaa gtagacattt tacgaatgct catcaaggcc aaagacgaat
541 acacaaagtg taaaacctgt tctgagccaa aaaagataac cagttcctct gccatctatg
601 acaatccaaa tctcatcaaa ccaattccag tgaaacccag tgaaaaccag caacagcgta
661 tacctcagcc caaccagacc ttagaccctg aaccccaaca cttatccttg acagctctgt
721 ttgggaagca ggacaaagct acatgtcagg aaactgtgga gcctccgcag actctccacc
781 agcagcagca gcagcagcag cagcagcagc aagagaagct tccaattagg cagggggttg
841 tacgctccct gtcctatgag gaacccagaa gacactcacc ccccattgag aagcagctct
901 gtccagccat tcagaaactc atggtcagga gcgcagacct ccacccattg tcagagctgc
961 ctgaaaaccg gccttgtgaa aatggcagta cccattctgc gggagaattt tttacaggac
1021 ctgtccagcc agggtctcct cacaacattg gaacttctcg tggtgtacaa aatgcttcca
1081 gaactcagaa cctgttcgag aaacttcaga gtaccccagg ggcagcaaac aagtgtgacc
1141 ctagtacacc agcacctgcc agctcagctg ccctgaaccg cagcagagct cccacttctg
1201 tcacccctgt ggctccagga aagggtctgg ctcagccacc acaggcctat ttcaatggct
1261 cccttccacc tcagacagta ggacatcagg ctcatggaag agaacggtcc acactcccaa
1321 gacaaacact ccccatctct ggtagtcaga ctggcagctc tggagtgatc tcccctcaag
1381 agttactgaa gaagcttcag attgtacagc aggagcagca gctgcatgcc tctaaccggc
1441 cagccttggc cgctaagttt cctgtgctcg ctcagagctc tggaacaggg aaacccttgg
1501 aatcctggat caacaagaca cccaacacag aacagcagac tcctcttttc caggtcatct
1561 cacctcagcg catccctgct acagcagctc cgtctctgct tatgtccccc atggtgttcg
1621 cacaacccac ctccgtcccg ccaaaggaaa gggagagcgg cctcttgcct gtgggaggcc
1681 aggagccacc tgctgctgcc accagcctcc tcctgcccat acagagcccg gagccctccg
1741 tgatcaccag cagcccactc accaagctcc agctccagga ggcactgctg tacctcattc
1801 agaatgatga caacttctta aatataatct atgaagccta tctcttcagc atgactcaag
1861 cagccatgaa aaagactatg tgacagcaag gccttttaaa actgattttc aaggtccttc
1921 tagaactccg gcacaaggtt ctttcatgtt gagtgttgtt tccttcaatg tttctgcctt
1981 ttttaaaaga aaagtatgtg taatatgaag taaaatgttt cagacttttt tttat
//