LOCUS AK056190 1718 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31628 fis, clone NT2RI2003344.
ACCESSION AK056190
VERSION AK056190.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1718)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1718
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003344"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012804"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 269 a 611 c 551 g 287 t
ORIGIN
1 agttcgcaaa gtccgggctt gcggccgggc ggcgcagagg cgcggaggct ccccgggcgg
61 ctgcatcccg gggctgcccc ggacatgccc cgcacccctt gtcggacgcc cgcgccgctt
121 caccggctgc cactccccgg agtgctctgg gactagagcg ccctccgccc caacttctga
181 ctttggggtc ccccagccct agaggaacgg gcgcccggct gggggagccc cgggcatggt
241 gtgacggggc agtcaggtca gctttgcggc gcccccaaac tccagccacc cgtcgtctcc
301 gtgccctcca gaatgtagct gctgaccccg cgccccccgg actccagcag cagtctccgc
361 cacctccggg ctccagcagc caactcttgt gtctctcagg aacccgccga cgtctccgcg
421 tcccccggat tccaggttcg cgacccccgc cgtcttcgcg cctccgggac tctagcagcg
481 gtctccgcga ccccctagga tccagccact gtacccgtgc tccccaaacc caggctccag
541 ccgtcgtctc cgcgctcccg gggatccagc tgtcgcgcca gtacccgcga ggacagcggc
601 accgcccaca ccccgcgcgc tcagagcccg gccgggcctc ggcgtggaga tgaacgcgcc
661 gctggacggc ctgtcggtga gctcgtcctc caccggctcg ctgggctcgg cggccggggc
721 gggcggcggc gggggcgcgg ggctgcggtt actgtctgcc aacgtgcgcc agctgcacca
781 agcgctgacc gcgctgctga gcgaggcgga gcgggagcag ttcacccact gcctgaacgc
841 ttaccacgcg cgccgcaacg tcttcgacct ggtgcgcacc ctgcgcgtgc tgctggacag
901 tccggtcaag cggcgcctgc tgcccatgct tcgtctggtc atcccgcgct ccgaccagct
961 gctcttcgac caatacacgg ccgagggcct ctacctgccc gccaccaccc cctacaggca
1021 gcccgcctgg ggcggccccg acagcgcggg gccaggggag gtgcgcctgg tgagtttgcg
1081 gcgtgccaag gcccacgagg gcttgggctt cagcatccgt gggggctcgg agcacggcgt
1141 gggcatctac gtgtctctgg tggaaccagg ctctctagct gagaaggaag gactgcgggt
1201 cggggaccag attctgcgcg tcaacgacaa atccctggcc cgggtgaccc acgcggaggc
1261 cgtcaaggct ctgaagggct ccaagaagct ggtgctgtct gtgtactcag cagggcgcat
1321 ctctgggggc tacgtcacca accacatcta cacctgggtg gacccgcagg gccgcagcat
1381 ctccccaccc tcgggcctgc cccagcccca cggtggtgcc ctgaggcagc aggagggtga
1441 ccggaggagc accctgcacc tcctgcaagg aggggatgag aaaaaggtga gtggggtggg
1501 gaaaggaggc cagcctctca gacaccgtat tctccctccg aacccagaac agcagagctg
1561 cttggaggcc gcaagaagag gctggttctg tccaggctct gtcttccctc aagtctgtac
1621 tgaagggtgg tgttttttct ttgcttttct ttttgacctg tgtagtgtat gttataatac
1681 aggctaagct gccacaataa aagagcttaa atactgtg
//