LOCUS AK056182 1912 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31620 fis, clone NT2RI2003184.
ACCESSION AK056182
VERSION AK056182.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1912)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1912
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003184"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012796"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 538 a 405 c 393 g 576 t
ORIGIN
1 atccttctct ggtgttcctt gttttagctg acgacactcg agagcgccaa ttttcctaga
61 ttcgattttg tggtttggaa ctgtggtttt ggaatccaga gtagtcgcct cgttctagta
121 tccaattgct ccagcaccaa ctggtgaaaa gaccatcatt cttctactca gttgcatttg
181 aagctttgtc aaaaaccaga cacaattgga gacacaggtt cttctatcaa ggccttgact
241 ggaatggcat attttcagat atgaccacat tgctttgagg aaatgaggct gatgttgcag
301 agctgataaa acccccacag aaaggccagc ctacctctgg gtctcactct gttccccagg
361 ctggagtgca atggtgcaat catagctcac tgccaccttg aactcctgga cttaagcaat
421 cttcctgcct caacctcctg agtagctggg accacaggtg tgtgccacca cgctcagctg
481 gatttagcta atattgaacc attgttccta acactgattt aacaaatact gaaccattac
541 tgccaagtgg ttcagaatct actaaattag tgtttgctgc aactttacag aacataacta
601 ctgggaataa tgaaaatcaa cttcattcgt gcatgtgtgt gtttattagt gattgaaagg
661 tggctcccaa aaagatatgt tcatattcta attcatggaa ccttattcgg aaaaaggttc
721 tttgcagatg taattaagat gaaattatcc tggattatca ggataggccc taaatccaat
781 gacaagtgtc cttataacaa acaactaaag acagtaaagg agaagaccat gtgaatttga
841 agagtgttat cgtttcaagc cgaggaacac ctggagcaac cagaagctgg aagatgcaag
901 ggaggattct ccccagagcc ttcagagagg cacaactctg ctgacacctt gattttggac
961 ttctggctgc tagaactgtg aaataataag ttactgctat ttgaagccac taagtttgtg
1021 gtaatttgtt acagcagcct ttggaaactt atacacagac acagacacac acacacacac
1081 agaaacacac acacacaaat agttaacgaa tatgttttta ttttgtttgt tttttgtttt
1141 tttttttgag acagagtctt gctttgttgc ccaggctgga atgcagtggc gtgatctcag
1201 aattcactgc aacctctgac tcccaggttc aagagtttgg aaacttatac acagacacag
1261 acagacagac acacacacac agacacacgc acacacacac acagttaaca aatatttttg
1321 ttttgttttg tttttgtttt ttttggtttt tttttggaga caagagtctt gctttattgc
1381 tcaggctgga gtgcagtggc gtgacctcag aattcactgc aacctctgac tcccaggttc
1441 aagagattct cctgcctcag actcccgggt agctgggatt ataggtgtgt gccatcacta
1501 ccagctactt tttgtatttt tagtagagat ggagtttcac cgtgttggcc aggctggtct
1561 cgaactcctg atcttgtgat ccatccacct tggcctccca aagtgctgga ttacaggcat
1621 gagccactgt gcccggccta caacaaatat gctatttttt aggggagagc taacattttg
1681 ttccacattc atcatttatt agtgtgaatg acagaaagca aatgtgactt ttctctttga
1741 aaagtaacta gtaactgggt caaggctgga atctaataca ctgtttagct gattgttctt
1801 ttgtatgttt cctctcaaat gactgcaaca cagtgaagaa aatgattata cagcttttgt
1861 taatatatgt tagagttggc tgccttaaga catcaataaa tactgaagag at
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