LOCUS AK056181 1854 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31619 fis, clone NT2RI2003154.
ACCESSION AK056181
VERSION AK056181.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1854)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1854
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003154"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012795"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 84..1538
/codon_start=1
/protein_id="BAB71114.1"
/translation="MAKPKPQTHAGPSCVGSAKLIPHVTSAISTELDPHGMSASPSVI
SRPIVQKTARVSLASPNRGPPGTHGTNQQVAMQMPVSTSHPNKQISIPLSALQLPGQD
EQVASEEFLSHLPSQVSSCEVALSPSVNTEPEVSSSQQQPPVAPAITTEATAQCIPAY
STKLNKFPVFNINDDLNDLCTSAVSPNTTKATRYALNVWRYWCMTNGLKDHTDITKIP
AVKLNELLENFYVTVKKSDGSDFLATSLHAIRRGLDRILKNAGVGFSITSSTFSSSTK
KLKEKLWVLSKAGMSGARSRNIVYFSLSDEEEVWQAGCLGDDSPITLLSTVVKYNSQY
LNMRTLQEHADLMYGDIELLKDPQNQPYFARTDSVKRESRSGSTRVCHGKIYHEHSRG
HKQCPYCLLYKYMYIHRPPTQMEAKSPFYLTARKEATDMGSVWYEEQRMGLRSLRGIV
PNLAKKVKLENCENFTFVSFTQVSRRLGSHSCCQ"
BASE COUNT 443 a 541 c 462 g 408 t
ORIGIN
1 aagaaatccc cgaagattat tacattgtgg caaatgcaga actgacagga ggagtagatg
61 gaccagccct gtccttgaca cagatggcaa aacccaagcc tcagactcac gctggtccct
121 cctgtgtagg gtctgctaaa ctgattcccc atgtcacatc tgccatcagc acggagctag
181 acccacacgg tatgtctgca tccccctctg tgatctccag accaattgtc cagaagactg
241 ctagggtatc tctggcttca ccaaacagag gaccccctgg tacacatggc accaaccaac
301 aggtggccat gcaaatgcct gtgagcacat cccatcctaa caaacagatc agtatcccct
361 tgtctgccct gcagctgcct ggacaggatg agcaagttgc ctctgaagag ttcctgtccc
421 atctgcccag ccaggtctcc tcctgtgagg tagccctttc tccctcagtt aacacagagc
481 cagaagtgag ctccagtcag cagcagcccc cagtcgctcc agccataacc actgaggcca
541 cagcacagtg cataccagct tattccacta agctcaacaa atttcctgta tttaatatta
601 atgatgactt gaatgatctg tgtaccagtg cagtaagccc aaatactacc aaagccacgc
661 ggtacgcctt gaatgtgtgg cgttattggt gcatgaccaa cgggctcaaa gaccacacag
721 acatcaccaa gatccctgca gtgaagttga acgagctgct cgagaacttt tatgtcaccg
781 tcaagaagag cgacggctcg gacttcctgg ccacctcgct ccatgctatt cgccgaggcc
841 tggaccgcat cctgaagaat gcaggtgtcg gcttttccat caccagcagc accttcagct
901 cctccaccaa gaaactcaag gagaagctgt gggtgctgag taaggcaggc atgtcgggcg
961 cgcgttctcg caacatcgtc tacttctccc tttctgacga ggaggaggtg tggcaggcag
1021 ggtgtctggg ggatgacagc cctatcactc tcctgtccac tgtggtcaag tacaacagcc
1081 agtacctgaa catgcggacg ctgcaggagc atgcggatct gatgtatggt gacatcgagc
1141 tgctcaaaga cccccaaaac cagccctact ttgcccggac ggacagcgtc aagcgggaga
1201 gtcggagcgg ctccaccaga gtgtgtcacg ggaagatcta ccatgagcat tcccggggac
1261 acaaacagtg cccttactgc ctcctctaca agtacatgta catccaccgg ccgcccaccc
1321 aaatggaggc caagtccccc ttctacctga ctgccaggaa ggaggccaca gacatgggca
1381 gcgtgtggta tgaggagcag aggatggggc tgcgctctct tcggggaatt gtcccaaact
1441 tagccaagaa ggtcaagctg gaaaactgtg agaacttcac ctttgtctcg ttcactcagg
1501 tctcccggag gcttggctcc cacagctgct gccagtgagc ccccactggg gcccggccac
1561 tgccctgtca cctgctcggg ccagccaggg ttggagcagc tggagctcct tggaggcagg
1621 ggctgaccag gtgtgacctc ccggtctggc ggctctcccc tggtgtggcc tgccctccct
1681 ttgacttggg gtttgctttt taaaatgaaa ctagatgagt ctaaatcatt cggatggttt
1741 atccaaatgt gcgtcaactt cgttagcttt tagaatctaa cacaatgtat aattgttaca
1801 tacaagagtg aggaaattca ttttctctag tgcctttcta gcacaggttt tctc
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