LOCUS AK056178 2729 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31616 fis, clone NT2RI2003019.
ACCESSION AK056178
VERSION AK056178.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2729)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2729
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2003019"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012792"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 717 a 563 c 627 g 822 t
ORIGIN
1 tacactgcat tcctggccag tcgcatttgt tttaatgcag gcatggccac agctctccta
61 gagaattatc tcaaagacca gaagggacct ggagaggcct atttcttagg tttttccagt
121 tggacaagga aggagtggtt tcactcagct tctagaagga gttggagcct aagtttatct
181 gcctccggga gctgcttgct ttgttttggc tccgaagagg tatcagatag ttttgacacc
241 tcaggaaact tgaaccaagc tgtgaaacca agacctccct acgtgaaaat caaggtggtc
301 tccttgtggc ttcaccagga tgtttgtgtc aggctgtctc agcagggtgg ggaatgacca
361 gccagggagc acagtgagcc ttactcagca ctggggagcg cactggtgag gcaaacccat
421 gaacctcaag aactgggagt atgttccttc agggagaagt tctggcccat tgcacaaaca
481 cttggaaatt aacttttccc taaattcaag atagtgtggt gtcggaagga aatgggacag
541 taaattagga gacgcgggct ccacctttac cttactacgt ggcctttgat gagctggctt
601 aacatccctg agtgattcca tgatagagat ctatactcca aactttattc ctggtatcta
661 gttggcttca ctgccacaga cactgtactc tctcttttta gaagtttttt tccttttttt
721 tccccctcaa ttggcttatt tagtaaattt aggtctgaat gaattggtac ctaaaagctt
781 ccaaatctat acatttcaga atatgggaat tttcttcctc ttcttccccc atatcccaac
841 atggagttct ggaaaactgt gcctcttccc ctgttctgcc ttcatggggg agagactgga
901 tgaaatctac aaaaacagcc aaaagtgcca ccctggcttc atgtcttgaa tttctaactt
961 gctcttggca aaggtcgctt tattttttaa tttttttcct tgcattttct tttttattct
1021 attgctgctg caaaaattaa ggcaaaagta gctttcgatc tttcatattt catcctggtt
1081 tcacaaggag tcacttatct taggaggtct gtaagtcagg ttacaaggcc gggagcggtg
1141 gctcacacct gtaatcccaa cactttgagc ggctgaggtg ggtgtatcac ctgaagtccg
1201 gagtttgaga ccagcctggc caacatggtg aaaccccgtc tctactaaaa atacaaaaaa
1261 ttggctgggc atggtggtgg gtgcctgtaa tcccagctac ttgggaagct gaagcaggag
1321 aatcacttaa acccaggtgg cggaggttgc agtgagccga catcgcgcca ttgcactcca
1381 gcctgggtga cgagtgaaac tccatctcaa aaataaaaat tgaaaaatca ggttacaaaa
1441 caccattttt cccgaaataa gacaataaga ggcttttctg tgaattcctt tatattgagc
1501 ctttcagaat tctccctggg tgggcaattt ctttaaatag tatttgaccc tcagatcaat
1561 cctgggaatt ttttccattt gggtagcaaa agctagagta ttgctgtggc gattataata
1621 cttttaaaaa gttttaccat tttaaagttg ccaacattta attaaggttt tcctttgaag
1681 cctcctttaa tttagggagt aaaatgttag ctaaaccaat tatatactat atactataca
1741 ctgtatctcc tgtggccatg agaggtgtgg ctataccgaa cagaaacatg cctactgttc
1801 aggaaagatg tcagttctgg taacacctct ctgtattggg atctgttaat tttgtaaatc
1861 taaattcttc tgctcttggc caggtgcagt agttcatgcc ctgtaatcct agaactttgg
1921 gaggctgaag ggggggcgga tcatttgagg tcaggagttt gagaccagcc tggccaacat
1981 ggtgaaaccc catctctact aaaaagtaca aaaattagct gggtgtggtg gtgcgtgcct
2041 gtaatcccaa ctacttggga ggctgaggca ggagaatcgc ttgaacctgg gaggcagcgg
2101 ttgcagtaag ccaagactat gccactgcac tcccgcctgg gtgacaaagc aatattctgt
2161 gtcaaataaa taaattcatt cttctgctct cctgacttag agaaatggtt tgcttaaaat
2221 gctagtaaca aacatcacag tcaacaggag cttgcttcat gcgaaggatc aatgtgattt
2281 gtggatggag atgatggtga tgaaattcct gtttcatggg gctgtttttc ttttcatctc
2341 actgggcagc aggtttagtg aggcagtgag atgctgctgc tgtggattct tgtagctatg
2401 cctcggcttc ttggcatatc aggtaggaac ctgttacaag tgaaatactt gaaacctctc
2461 tgaccaagag cctctgatgg agtgggaggt gagctaattc tctgaccagc ttggggcact
2521 gtttcagcca ctggtcacat tccttgcttc aaactgaaat tcagtttggc tttgagtata
2581 gggatacatg gtggattcat gtacttcagt gtttgttttg accaaagttt atttttctag
2641 tgcattttct aagtcaaagt ggtgaaaata tgtaataatt ttagtatgca tgactcagtc
2701 tgaaacaata aaaatctctg aaaaatgtg
//