LOCUS AK056171 2222 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31609 fis, clone NT2RI2002852.
ACCESSION AK056171
VERSION AK056171.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2222)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2222
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2002852"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012785"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 120..692
/codon_start=1
/protein_id="BAB71110.1"
/translation="MIISSNADRLLDPQKLEDRSAPWLTWACQSCRAEPSPPEDCTGL
NCAYKESLQINRNFSSRAGIGRAGMEMRLWNERSLSTWGKWHFTRNIKYLGGRTCEGQ
GTPRGGTYLPLRKCGCSEGLASLVQKRTEAHPQQTFLLPDPQRPLPGGGGRSKENRLR
VTTYQASPGLVRIRSPGGARTLSPLCNQAV"
BASE COUNT 596 a 581 c 585 g 460 t
ORIGIN
1 gcatagaagt aagaaatcag gagcaaaggt tgggggtggg gaggaagcaa aattgtcctc
61 gcagcagaga atgcctaagc caggaggaac ttgtaaatga aaaccgctcg cattagcaaa
121 tgattatttc ctccaacgct gaccgtctct tagacccaca gaaactcgag gaccggtctg
181 caccctggct gacttgggcc tgccagagct gccgggctga gccctctcct ccagaggact
241 gcactggcct gaactgtgct tataaagaat ctctgcaaat caacaggaac ttctcatcca
301 gagccgggat agggagggct ggaatggaaa tgaggctgtg gaatgaaagg tcactctcaa
361 cttggggaaa atggcatttc accaggaaca tcaagtacct cggaggcaga acatgcgagg
421 ggcagggaac gccaagagga ggcacttacc tgccattgag aaagtgcggc tgctcagagg
481 gcctagcctc tcttgttcaa aagagaacag aggctcaccc acagcagaca ttcctgcttc
541 cggatcctca gcggcctctc ccagggggag gaggaagaag caaagagaac aggctaagag
601 tcaccactta ccaggccagc cccggtcttg tacgcattag gtcgcccggc ggggcaagga
661 cactgtcacc tctttgcaat caggcagtct gaaacccagc gacttgcctt catgtccccg
721 ggctttcgcc tataatttat caccctcaac agcagctcgg ctgagctgaa agtctccggt
781 gcagtgcacc gaagagccat tgatttcgtg ggattgtgaa taaatacgag gaactgcagt
841 tgttctgctg gccgaggtga caatatcaga agggagcctg gggggaggga aacagctgtg
901 gtttgcattt tgaagttgtt tttaacattt ttagtaattg tttcagattc tccagttctg
961 atgcccctct cctagaaatg agaaaaccca tcttttatac ttgaggtata atcctttccc
1021 actagacaca gcctcctcca cccttctctc aataatgcag aagtggtgat taaagggcac
1081 attcatatgt aaaaggcgaa cttccaaact cgcctggcag gacagaaatt gcttcaatct
1141 acagaaaaga ggctgattta caattgaatt cagtgtttag ccctcaacaa ctagaggcaa
1201 tagcaaccgc ggccctttct cctccccagc tgggatgagg ccctctggaa aaacgacaac
1261 atcgcaacag gaatttcctg gatcaagaat gccaagttct ttccaatttt actgaggcag
1321 gatccgactc cctgtgagaa cgcactgccc accacccacc caccccctca gcaggcccag
1381 gccccaaagg ctccctccaa catgtgtaca tgaggccctt caaactcgac cggaacacca
1441 caaagaatgt ggagaaaccg gttgcctctg gcctttacat tgtggctggc tgaggagctg
1501 gttctggtgg ctgacacccg ttggcactat ttatccagcc cctgcctggc ttgggttctg
1561 gaaatctccg gtggtagtgg cttccaaact gcaatgggga atcgcggcgt cagagggcaa
1621 ttcagaaaca aaaagacagt tctgtgctgg cagcatttga agcacagaag tgggttggga
1681 gaagcaaagg gaaattagct tcagaaaggg ggcagatgaa ggaagaggcg ccactgctaa
1741 ccaaccaagg tccccatctg ccctggatgg tatctctaaa agccacctaa agaaggcaaa
1801 attctctact gaaacgccag gtacgaaggg acaggccctc cacagtcacc ttgtcacatt
1861 cctttgggga gcgaagagtt cagacccaac atgtcaccca aattaccctg gccagccacc
1921 acgtaaagat gctgcggctg tgcatggtgg ctcacgcctg tagtcccaac acgttgggag
1981 gccaaggcgg gcggatcacc tgaggtcagg agttcgagac cagcctggcc aacatggcga
2041 aatcctgtct ctattaaaaa tacaaaaatt agccgggtgt ggtggcacac acctgtggta
2101 ccagctactc ctactgggga agctgaggca ggagaatcgc ttgaccccag gaggctggag
2161 tgcagtgagt cgagatcacg ccattgcact ccagccgggg tgacagagca agatgccatc
2221 tc
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