LOCUS AK056149 1913 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31587 fis, clone NT2RI2002243.
ACCESSION AK056149
VERSION AK056149.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1913)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1913
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2002243"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012763"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 1..1350
/codon_start=1
/protein_id="BAB71104.1"
/translation="MSDIRHSLLRRDALSAAKEVLYHLDIYFSSQLQSAPLPIVDKGP
VELLEEFVFQVPKERSAQPKRLNSLQELQLLEIMCNYFQEQTKDSVRQIIFSSLFSPQ
GNKADDSRMSLLGKLVSMAVAVCRIPVLECAASWLQRTPVVYCVRLAKALVDDYCCLV
PGSIQTLKQIFSASPRFCCQFITSVTALYDLSSDDLIPPMDLLEMIVTWIFEDPRLIL
ITFLNTPIAANLPIGFLELTPLVGLIRWCVKAPLAYKRKKKPPLSNGHVSNKVTKDPG
VGMDRDSHLLYSKLHLSVLQVLMTLQLHLTEKNLYGRLGLILFDHMVPLVEEINRLAD
ELNPLNASQEIELSLDRLAQALQVAMASGALLCTRDDLRTLCSRLPHNNLLQLVISGP
VQQSPHAALPPGFYPHIHTPPLGYGAVPAHPAAHPALPTHPGHTFISGVTFPFRPIR"
BASE COUNT 378 a 582 c 526 g 427 t
ORIGIN
1 atgagcgaca tccgccactc gctgctgcgc cgcgatgcgc tgagcgccgc caaggaggtg
61 ttgtaccacc tggacatcta cttcagcagc cagctgcaga gcgcgccgct gcccatcgtg
121 gacaagggcc ccgtggagct gctggaggag ttcgtgttcc aggtgcccaa ggagcgcagc
181 gcgcagccca agagactgaa ttcccttcag gagcttcaac ttcttgaaat catgtgcaat
241 tatttccagg agcaaaccaa ggactctgtt cggcagatta ttttttcatc ccttttcagc
301 cctcaaggga acaaagccga tgacagccgg atgagcttgt tgggaaaact ggtctccatg
361 gcggtggctg tgtgtcgaat cccggtgttg gagtgtgctg cctcctggct tcagcggacg
421 cccgtggttt actgtgtgag gttagccaag gcccttgtag atgactactg ctgtttggtg
481 ccgggatcca ttcagacgct gaagcagata ttcagtgcca gcccgagatt ctgctgccag
541 ttcatcacct ccgttaccgc gctctatgac ctgtcatcag atgacctcat tccacctatg
601 gacttgcttg aaatgattgt cacctggatt tttgaggacc caaggttgat tctcatcact
661 tttttaaata ctccgattgc ggccaatctg ccaataggat tcttagagct caccccgctc
721 gttggattga tccgctggtg cgtgaaggca cccctggctt ataaaaggaa aaagaagccc
781 cccttatcca atggccatgt cagcaacaag gtcacaaagg acccgggcgt ggggatggac
841 agagactccc acctcttgta ctcaaaactc cacctcagcg tcctgcaagt gctcatgacg
901 ctgcagctgc acctgaccga gaagaatctg tatgggcgcc tggggctgat cctcttcgac
961 cacatggtcc cgctggtaga ggagatcaac aggttggcgg atgaactgaa ccccctcaac
1021 gcctcccagg agattgagct ctcgctggac cggctggcgc aggctctgca ggtggccatg
1081 gcctcaggag ctctgctgtg cacgagagat gacctgagaa ccttgtgctc caggctgccc
1141 cataataacc tcctccagct ggtgatctcg ggtcccgtgc agcagtcgcc tcacgccgcg
1201 ctccccccgg ggttctaccc ccacatccac acgcccccgc tgggctacgg ggctgtcccg
1261 gcccaccccg ccgcccaccc cgccctgccc acgcaccccg gccacacctt catctccggc
1321 gtgacctttc ccttcaggcc catccgctag gctggcccgt gtgtgccttc tgcgctctcg
1381 ctggacgaag cctttcgaga tggaaggggt ggccggactc ccagaagaga acctcgggga
1441 aggggtcggg cagcccctcc ccgccggcag aaccgtcttg gtgtcacgga gtccaggtgc
1501 ttcccacccg gtcgcattct ttgacatgca gattggatgg tggagggaag agtccagcct
1561 ctgccgagag cctgctgcgt gcatttttaa aagatgccga tcctgggagc ctctgttctc
1621 tgcgcatttc agacacagcc tgtgtggcga ggagtgtgac ggcaggagcc acgggtgcaa
1681 gcccgtgtgt ctggcctctt tcctcgtgaa gacgatgtgt ccccgccaga aaaagtgggc
1741 accttctgca gccccgtgag ctgagcccag gctgcgtagt gaccacaagc ttatgtgcag
1801 cactgctcag ggaggctgtc aggaattccc ctcacctcgg aaaggaactt ctcagtttta
1861 ttgggggtgt ctaaatttcc tttcatatgt tcaaataaat ttttctaaac agt
//