LOCUS AK056142 2453 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31580 fis, clone NT2RI2002041.
ACCESSION AK056142
VERSION AK056142.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2453)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2453
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2002041"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012756"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 398..850
/codon_start=1
/protein_id="BAG51632.1"
/translation="MRYACSSSEDWPPPLDISSDGDVDATVLRELYPDSPPGYEECVG
PGATQLYVPTDAPPPYSLTDSCPTLDGTSDSGSGHSPGRHQQEQRTPAQGGLHTVSMD
TLPPYEAVCGAGPPSGLLPLPGPDPGPRGSQGSPTPTRAPASGPERIV"
BASE COUNT 564 a 764 c 642 g 483 t
ORIGIN
1 ggactgggag ccggagccgg ggcgggaacc ggagctggag ccgaagctgg agccggcact
61 gcggcgcacg gagcggagcg ctgcagccaa gccgatagca cgatacaacc gcaccagcta
121 cttctacccc acattctcag agagctcgga gcacagccat ctgctcgtgt cccccgtgct
181 ggtggcgagt gccgtcatag gtgtggtcat catcctctcc tgcatcacca tcattgtggg
241 cagcatccgc agggacaggc aggcccggct tcagcggcac cgccaccgcc accaccgcca
301 ccaccaccac catcatcacc accgccggcg tcgacaccga gagtacgagc acggctacgt
361 gtcggacgag cacacataca gccgctcaag ccgcaggatg cgctatgcct gcagctcctc
421 agaggactgg cccccaccct tggacatcag ctctgacggg gacgtggatg ccacggtgct
481 cagggagctg tacccagatt ctccaccagg ctacgaggag tgtgtggggc caggggccac
541 tcagctgtat gtccccacgg acgcaccacc accctactcg ctgactgatt cctgccccac
601 gctggatggc acctccgact caggcagcgg ccacagccct ggccgacacc agcaggagca
661 gaggaccccg gcccaaggtg gccttcacac ggtctccatg gacacccttc ccccctacga
721 ggctgtgtgc ggggctggcc ccccatcagg cctgctgcca ctgccgggcc cagacccagg
781 gccaaggggc tcccagggct cacccacccc aacccgggcc ccagcctctg gcccagagag
841 gattgtgtga gggacccagc cagccgggtc ctgctggtcc ctacaggctg aaccacattc
901 tttaggcaca caaaggcgtg cacacacaca cagagatgca cacgtgactc ataacacaca
961 catagaccaa acttgtatac acacagacat ctacactgac ataccccatg tacacacaca
1021 gatctagacg tgctccacat atgtgtgaat atgcgcacat acaggcccca gtgaggttcc
1081 gttgtgcgct gtgcctatct ctcgattcca gggcagatga gccacaacat caccaccctg
1141 ccacttacaa ggtgggggac ctgggtctgg ggtctcaggc gcaaactgga ggccctcaca
1201 gcccactagg ccccctccca accccagtac cctcagtccc tcagtcaggt ggtgctagta
1261 gagctatctc tgacgctgca ggccccaggt agatgggcag ggccctccgt gggtgtgcag
1321 cggcggttcc ctcatcagtc cagccactgc ccaggactaa gggtcttcaa ggaagatgag
1381 gccattgccc ttctccaggt ggcttcacca cagtgctgcc tgggcacctc tctgaggcag
1441 agaagtggcc cctccctcag tccctgcctg gccgaccact gtggcctgca cgcgaatggg
1501 tcagagccag accccggagc tggtgctgtc cttctaggtg gctgctcata gctggggatg
1561 gtgagtggct gtgttctggc cttgcccacg tgggtgtttg cagagacaaa atgaagggga
1621 tatgctcccc ataaatacca ccttgccacc acaagaggac cagaaggcta ctcagccttg
1681 ctgccacaga aggggatttg tagggtcagc ctggctgcag aaaggctagc tcagatcttg
1741 accaagaaac cccagcgatt tccatcaaag accacgcagg taacagaggt gattcctgaa
1801 gacccagccc acagagggtg gcagccaagc atctctgagc agtgtccctg ctagcatcgc
1861 cagcggccat cactctgcct ccctcccacc tcttgtccct cgtcgttaaa aaacagcacc
1921 ctatcctgct tccccacatt tctgttcctc caatgaaggg ctaagactat ttagtaatct
1981 ctttcttaag cagaggagtg gcaaggatgg caatcttgaa ttttattttc tgtagagata
2041 gcatttcttc tggtgcggag ctgaaaggaa tccacccaga agttctgtag catcctgcgt
2101 gcagcctcct ggagccccag actccatctg ggggagggac ttgtttacaa gcagttctga
2161 ccaccttagt ggtgtactgt tttctaggca aaaaatatct gtctgttgta ctgtatagcc
2221 tttaaaatgc agtccaggaa tgagactctt ttaagaaaca catcctgctt ctgcaattcc
2281 agagagtgct gggggaaaaa aagggataaa aattcctacc tactcatcag tgtttgaaag
2341 atggagctga atagcttttc ttgttcctgg actaggcaat gaataagcaa caatgtatct
2401 tttctgtgtt caaaataaat acataatatc aataaaatgt agcaagaagt aac
//