LOCUS AK056140 2679 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31578 fis, clone NT2RI2001952.
ACCESSION AK056140
VERSION AK056140.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2679)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2679
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2001952"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012754"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 745 a 571 c 519 g 844 t
ORIGIN
1 cttcagtttg tttgttttta actttcaaga tgcatgtttt gttttgtttt gctttgtttt
61 tgtctcttaa gccaattcat ttttctaaca gttgaaattc ttttagtact aaagaaatag
121 atactgctct tttatggctt tagtgtttat atacctgttt atatgcatac attaacaaaa
181 ttagtaacag cagtgcttag aatttcaatg ctgtgtcata ctgatgatta actccatact
241 cctagtggtg atgtggtcca gatactggaa tggaagtggc aggatgtgga gtccagacat
301 agtctaccac tcatgctact tactagtaat attaaggtgt taaacttagg aatttgtcac
361 atgtaggttc ctgaggagat ctaaaagtta atacaaaacc aagctgttta ctgattttca
421 tgagaacatt taagttagtt taggtctacc tgatttattt taaaagaagg cagagtgctg
481 ggattacagg cgtgagccac gtcccccagc cagtttctta atctatacat ctagcccagt
541 ccatgcccct gtttcagact attatatcca accacctgct atacatctca taaaggtatc
601 tcaaacacag catatctaaa gctgaagtca tcatcttgcc ctggtttttc ctgtctcttt
661 gctgatggca tcccatttac ccagtcattc aaactggaaa tctaaaagcc tcacttagca
721 agagtttgga ggcagaatat gataaagaac aaggactctg ggaggccaag gcaggtggat
781 tgcttgaggt caagagttcg agaccagtct ggccaacatg gcgaaacccc gtctttgcta
841 aaaaaacaca aagaaattag ctggacgtgg tggcacatgc ctgtagtccc agctgtgtgg
901 gaggctgagg caggggaatt gcttgaacct gggaggtgga ggttgcagtg acccaagatt
961 gcatgcacca ctgcactcca gcctgggtga caaagcgaga ccctgtctca aaaaaaacaa
1021 ggactctaca gatagataga catcatatat catattagtt tgaatcctgg ctgttagctg
1081 ttagacttgg gcaaataatt taacctcaac tttccctatc tataaaatgg gaattaaaat
1141 atttgcacct tatagggtta ttgtgaggat taaataggaa cattgtataa agcacttaaa
1201 acagtacctg ggacatacta agtaatataa ttgttaactc ttactagcat tctgtccctt
1261 ttatttccta aatatctctc atgcccatct cctaaatatc tctttcctaa atatctctca
1321 tgcttttggc ttaagacctc ttttcctcct tatctattga ctggactgcg gcaaatgcct
1381 gctaatcgtt atttttgtgt tcattcattt agcaagccaa tttattacgc gcttagggtg
1441 ctgccagggc tacaaaagct gttgagactg tacttgatat gaagaagctt gctgattatc
1501 atgggaagac tgacataagg aagcaccata aaattctgct gtatgagagg tatacacggg
1561 atactggggg ttaataattg agggtatggt ccattcaaca tgagtgagac agaaacaatt
1621 cataaaggag atgaaatgtc ttgaggaatg agctcttaga agaatagttt tcaaatgagt
1681 gtgcatcaca gtcacctgta agacttatta aaacagatcg ctgggcccta cacccagagg
1741 ctgtggttca gtaggctgta gtaaaccagt aatttgtatt tctatgacgt tcccaggttc
1801 taatgctgtt ccccaaggcc acaccttgga aaccaccaca ttaaaatacc cagaaggcat
1861 taattcccag tccttcctct acacagctgc aaaacaatgg tcctgaccat ttcatctttg
1921 cactacatcc ttcactgtct ctcttttgcc cataggataa gtacaaacta gatctggtta
1981 ctgcctgccc caccagcctc agcatctctc acaactagga ctaacttttt cttctgacaa
2041 ctataaaata tttcccttgc cttctcaagt ttgctcaagg tcaagttatg ccttttgcct
2101 ggaatgactt gacttctctt ttgttttact tagctggctg cttttcatct tgtaggttag
2161 gtcaaggact ccaggaagtc ttccctggac aagtaatgaa gagggcataa tccaagggcc
2221 aactcccatg tttggaacct gactccattt tcaggcacgt aatattgtca aattcctttt
2281 aaaagcacct gtctgtctgt taacgttggt gcagatactg ctattcccct cctccatacc
2341 attgctgatg gttactgagg gtatgggaag ggccgactag tccagctgtt cacaaacagc
2401 ccttaatgtc aaactgaata ctgccaacgt agttccagtt tctgtatcta aagactcagc
2461 ttggagtcac ttgtctggac taaaggtaac ccctccttgt ctggtttgtg actttctgta
2521 ctctgatgcc cccagctttc tgccttctag aaatttgtca gaatttccaa aattcttggg
2581 ccttccttct tgctctatat atggttttgg attcattcct tttaaaaaat atttactgtc
2641 atttcagtag aattttgaca caataaatat aagcacatc
//