LOCUS AK056127 1859 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31565 fis, clone NT2RI2001474.
ACCESSION AK056127
VERSION AK056127.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1859)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..1859
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2001474"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012741"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
BASE COUNT 509 a 412 c 316 g 622 t
ORIGIN
1 gtatgatgaa atggctcacc tagtagtttt cccagaacta aggaatagtc cttacccaaa
61 tatatttagg tgtatacttc ttgcaagaca taaaattgct tgcatctttg aaaaggcagg
121 ttttcagaag catcttcctc gtttgtatgc cttgaagaag aaattcagcc aggatacctt
181 ggtatggtaa gaaatatttt tgaacaaaag acatgttttt gaatctttta ctctagctac
241 catttcccct cacatttaga ctgtccccag aaagtgaatt ctctttctgc ttggaagcac
301 tgtgcttata gaagcaaccc tgctctgtaa ataatgctgt aatggtgcca cctgtcgggg
361 aatagggaca ttacaagggg agcctcaggg aatttatggc tttcacttca acaagcacta
421 tcagatgcct gctgaagtcc tggctctctg tcaaatggtg ggtggcaagg agagaagaga
481 aagtaaataa atagaatgta tatgaagaat gtaaaaaaca actgcaatat actcctgact
541 tgtctcccct ggccttgctc acataccttt catccgaagg ttaatcttcc taaaccagag
601 ctctgaacat tcattcttct cgaaatctgt gtgtccctag tgcttcttaa actaagttta
661 aaatccagtg ggcgtgggga gtcaaggtcc tccctaatta ggcccggaat attttttaac
721 ctatatattt catttctcaa ctacatcttc tagacaccat cccaccaggt aagaagccca
781 tatcatccca gtcaaagctg gaaatgattc ccaggggtat cccagtttct ttaatccctg
841 agtgagccat ctaaatgagc gccctctggc ctcaattccg tatctctcag tcatgcttcc
901 caagtgcagt cagatgcaca tctcctccag aaaaaggact tctgcagcct gggccacctg
961 caccagcctc tcagaggtgt tccccacctc cccttgcccc ttttccctct ctcacgctgt
1021 gaatgggaaa tccccagaaa tagcatgcaa aactttttga ctatgaattt gtgcttttat
1081 ttcaaggaat agggttcata attttcatca aattattaat gaagcctgta aagtcagaaa
1141 gggtaactaa ctctagttaa aatgtgcatg ctactcacca tttcccagaa atgcccctca
1201 ttttcctgca tctggttttc tggtcattct ctttactttc ctgctccatc atgtctctgt
1261 tagttagaac tctaccggtt ctctaaagcc tttctctaat gctacctcca tcatggagcc
1321 tttcctgatg tgatctcttc ctccttaaaa tgtccaaatc tttttctttg tacctatttg
1381 gagcatggta actgtatttt gccttacttc tattattcct catgtgcttt tatattctcc
1441 cttctggatt atcagttatc ttgagagctg gaactgcttt ctcattttta tagtctctga
1501 agtatctatt ttattgactt taaggcacaa ttgactgtat ggcatcctat tttaggtacc
1561 attaagaaag gaaaaaacac tgtcaaatta tcgctatgct ttcttatatt tatattttaa
1621 agtttcattc ttaaagaact cttttagagt tgcttagaaa tagaacttaa aaaaatcact
1681 actatgcact gaaataactg agtcattatg gtattattta atttttttca cactgagtct
1741 gacacttgaa tcactttttg actcagaata tttgatgtat tttcccatgc atttctattc
1801 tctgggccat tttgagtatt agtgaagtgg catttcttat aaagaaaaaa aagtcctct
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