LOCUS AK056126 2547 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ31564 fis, clone NT2RI2001450, weakly similar
to TRICHOHYALIN.
ACCESSION AK056126
VERSION AK056126.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2547)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2547
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2001450"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012740"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 451..2331
/codon_start=1
/protein_id="BAB71098.1"
/translation="MSKKGRSKGEKPEMETDAVQMANEELRAKLTSIQIEFQQEKSKV
GKLRERLQEAKLEREQEQRRHTAYISELKAKLHEEKTKELQALREGLIRQHEQEAART
AKIKEGELQRLQATLNVLRDGAADKVKTALLTEAREEARRAFDGERLRLQQEILELKA
ARKQAEEALSNCMQADKTKAADLRAAYQAHQDEVHRIKRECERDIRRLMDEIKGKDRV
ILALEKELGVQAGQTQKLLLQKEALDEQLVQVKEAERHHSSPKRELPPGIGDMVELMG
VQDQHMDERDVRRFQLKIAELNSVIRKLEDRNTLLADERNELLKRSRETEVQLKPLVE
KNKRMNKKNEDLLQSIQRMEEKIKNLTRENVEMKEKLSAQASLKRHTSLNDLSLTRDE
QEIEFLRLQVLEQQHVIDDLSLERERLLRSKRHRGKSLKPPKKHVVETFFGFDEESVD
SETLSETSYNTDRTDRTPATPEEDLDDATAREEADLRFCQLTREYQALQRAYALLQEQ
VGGTLDAEREARTREQLQADLLRCQAKIEDLEKLLVEKGQDSKWVEEKQLLIRTNQDL
LEKIYRLEMEENQLKNEMQDAKDQNELLEFRVLELEVRDSICCKLSNGADILFEPKLK
FM"
BASE COUNT 641 a 629 c 819 g 458 t
ORIGIN
1 agctgcagcc acccgcgtgc ggcacgcgcc ggccgcctgc ccggatcgcg gccaccagtg
61 ccgcggcctc tcgactcccg ccccgccgag ccgcgcgcgc gactgccatc ctcctcaatt
121 gctttttacg attttttttg tgcatgcatt cattttctgt tccgttttgt cctttttaag
181 gcggtggcgg cggcgacagc ggcgtaagct cgcagcgggg agggggcggc cggaggatgc
241 ggcgcggggc tgcgctcgct acgtccgctg ctgctgcccg gctcgggcct gagcgccgag
301 caggatccca agtgatggtg gtttcctcgg agggcgagct gagtcctgcg cgactggtta
361 gcacggtgga gctggtagcc acgcctgctg gctggcgtgc gtgaacaggt gtggaccgca
421 ggatctcagc actctgaccc aaggggaagc atgtcgaaga aaggccggag caagggcgag
481 aagcccgaga tggagacgga cgcggtgcag atggccaacg aggagctgcg ggccaagctg
541 accagcattc agatcgagtt ccagcaggaa aaaagcaagg tgggcaaact gcgcgagcgg
601 ctgcaggagg cgaagctgga gcgcgagcag gagcagcgac ggcacacggc ctacatttcg
661 gagctcaagg ccaagctgca tgaggagaag accaaggagc tgcaggcgct gcgcgagggg
721 ctcatccggc agcacgagca ggaggcggcg cgcaccgcca agatcaagga gggcgagctg
781 cagcggctgc aggccacgct gaacgtgctg cgcgacggcg cggccgacaa ggtcaagacg
841 gcgctgctga ccgaggcgcg cgaggaggcg cgcagggcct tcgatggaga gcgcctgcgg
901 ctgcagcagg agatcctgga gctcaaggca gcgcgcaagc aggcagagga ggcgctcagt
961 aactgcatgc aggctgacaa gaccaaggca gccgacctgc gtgccgccta ccaggcgcac
1021 caagacgagg tgcaccgcat caagcgcgag tgcgagcgcg acatccgcag gctgatggat
1081 gagatcaaag ggaaagaccg tgtgattctg gccttggaga aggaacttgg cgtgcaggct
1141 gggcagaccc agaagctgct tctgcagaaa gaggctttgg atgagcagct ggttcaggtc
1201 aaggaggccg agcggcacca cagtagtcca aagagagagc tcccgcccgg gatcggggac
1261 atggtggagc tcatgggcgt ccaggatcaa catatggacg agcgagatgt gaggcgattt
1321 caactaaaaa ttgctgaact gaattcagtg atacggaagc tggaagacag aaatacgctg
1381 ttggcagatg agaggaatga actgctgaaa cgctcacgag agaccgaggt tcagctgaag
1441 cccctggtgg agaagaacaa gcggatgaac aagaagaatg aggatctgtt gcagagtatc
1501 cagaggatgg aggagaaaat caagaacctc acgcgggaaa acgtggaaat gaaagaaaag
1561 ctgtcagcgc aggcgtctct gaagcggcat acctccttga atgacctcag cctgacgagg
1621 gatgagcagg agatcgagtt cctgaggctg caggtgctgg agcagcagca cgtcattgac
1681 gacctctcac tggagagaga acggctgttg cgctccaaaa ggcatcgagg gaaaagtctg
1741 aaaccgccca agaagcatgt tgtggagaca ttttttggat ttgatgagga gtctgtggac
1801 tcagaaacgt tgtccgaaac atcctacaac acagacagga cagacaggac cccagccacg
1861 cccgaagaag acttggacga tgccacagcc cgagaggagg ctgacctgcg cttctgccag
1921 ctgacccggg agtaccaggc cctgcaacgc gcctacgccc tgctccagga gcaggtggga
1981 ggcacgctgg acgctgagag ggaggcccgg actcgggagc agctacaagc tgatctgctg
2041 aggtgtcagg ccaaaatcga agatttggag aagttactgg ttgagaaggg acaggattcc
2101 aagtgggttg aagagaagca gctgctcatc agaacaaacc aagacttgct ggaaaagatt
2161 tacagactgg aaatggaaga gaaccagctg aagaatgaaa tgcaagacgc caaggatcag
2221 aacgagctgt tagaattcag agtgctagaa ctcgaagtaa gagactctat ctgttgtaaa
2281 ctctcaaacg gagcagacat tctctttgaa cccaaactga aattcatgta aagctctcag
2341 atgttttcaa gcatgtgtaa aggggacatg ttatagtttc tttctttctt tctttctttt
2401 tttttttaaa tctgtatgtt cagaataatt tcactgcctt aatgtgttct ggagagcgtg
2461 ctcacccaag tctatggaca tgtaccagag ctaatatatt tattgcctat ggcttgtttt
2521 gcacttaata aaataatttg tttttgc
//