LOCUS AK056100 2197 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31538 fis, clone NT2RI2000727, highly similar
to TRYPTOPHANYL-TRNA SYNTHETASE (EC 6.1.1.2).
ACCESSION AK056100
VERSION AK056100.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2197)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2197
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2000727"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012714"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 620..2035
/codon_start=1
/protein_id="BAG51626.1"
/translation="MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLV
SLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDK
LIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYT
GRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVEN
AKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIG
KISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPAL
LHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGG
NCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRK
EVTDEIVKEFMTPRKLSFDFQ"
BASE COUNT 570 a 597 c 573 g 457 t
ORIGIN
1 tgtcaagccc tatgctgggt ctggtgtggg tggaaatcaa acacaaaaca cagatccccc
61 cggaggcccc agagcgatga aagcaacaaa caagatacga ccagagatgg gttttgaagg
121 atgaatagga gttagctgag ctgtcaatag taagaagtaa ggttggtcca aggcacagcg
181 ctttctgaag gaacaggaag tgaaaggaag gcgagcggct atgagagacc gcgttaagcg
241 gcatggggcg ctttaggcgc tgcccagtcc cggcccctcc agcccggggc ccgccgctca
301 ccacacgcac cggaagcgct cggttctccc ggaggcagac cggccagcac actaccatcc
361 agtcacgttc cgacgcgacg ccgcggccgc cagccaatca gtggcggcct ctgccccgcc
421 cccgcgcgaa cagccaatga cggcgccgtc gggccggaag ttgttccgtc cggacggcgt
481 ttccacggac gcccaggaga cagccggttg ctgagccggg agcgctgact ggcccggctg
541 ggcaggtctt gactcgtctg ctgaacaaat cctctgacct caggccggct gtgaacgtag
601 ttcctgagag atagcaaaca tgcccaacag tgagcccgca tctctgctgg agctgttcaa
661 cagcatcgcc acacaagggg agctcgtaag gtccctcaaa gcgggaaatg cgtcaaagga
721 tgaaattgat tctgcagtaa agatgttggt gtcattaaaa atgagctaca aagctgccgc
781 gggggaggat tacaaggctg actgtcctcc agggaaccca gcacctacca gtaatcatgg
841 cccagatgcc acagaagctg aagaggattt tgtggaccca tggacagtac agacaagcag
901 tgcaaaaggc atagactacg ataagctcat tgttcggttt ggaagtagta aaattgacaa
961 agagctaata aaccgaatag agagagccac cggccaaaga ccacaccact tcctgcgcag
1021 aggcatcttc ttctcacaca gagatatgaa tcaggttctt gatgcctatg aaaataagaa
1081 gccattttat ctgtacacgg gccggggccc ctcttctgaa gcaatgcatg taggtcacct
1141 cattccattt attttcacaa agtggctcca ggatgtattt aacgtgccct tggtcatcca
1201 gatgacggat gacgagaagt atctgtggaa ggacctgacc ctggaccagg cctatagcta
1261 tgctgtggag aatgccaagg acatcatcgc ctgtggcttt gacatcaaca agactttcat
1321 attctctgac ctggactaca tggggatgag ctcaggtttc tacaaaaatg tggtgaagat
1381 tcaaaagcat gttaccttca accaagtgaa aggcattttc ggcttcactg acagcgactg
1441 cattgggaag atcagttttc ctgccatcca ggctgctccc tccttcagca actcattccc
1501 acagatcttc cgagacagga cggatatcca gtgccttatc ccatgtgcca ttgaccagga
1561 tccttacttt agaatgacaa gggacgtcgc ccccaggatc ggctatccta aaccagccct
1621 gttgcactcc accttcttcc cagccctgca gggcgcccag accaaaatga gtgccagcga
1681 ccccaactcc tccatcttcc tcaccgacac ggccaagcag atcaaaacca aggtcaataa
1741 gcatgcgttt tctggaggga gagacaccat cgaggagcac aggcagtttg ggggcaactg
1801 tgatgtggac gtgtctttca tgtacctgac cttcttcctc gaggacgacg acaagctcga
1861 gcagatcagg aaggattaca ccagcggagc catgctcacc ggtgagctca agaaggcact
1921 catagaggtt ctgcagccct tgatcgcaga gcaccaggcc cggcgcaagg aggtcacgga
1981 tgagatagtg aaagagttca tgactccccg gaagctgtcc ttcgactttc agtagcactc
2041 gttttacata tgcttataaa agaagtgatg tatcagtaat gtatcaataa tcccagccca
2101 gtcaaagcac cgccacctgt aggcttctgt ctcatggtaa ttactgggcc tggcctctgt
2161 aagcctgtgt atgttatcaa tactgtttct tcctgtg
//