LOCUS AK056097 2343 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ31535 fis, clone NT2RI2000685.
ACCESSION AK056097
VERSION AK056097.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2343)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Ninomiya,K., Wagatsuma,M., Kanda,K., Kondo,H., Yokoi,T.,
Kodaira,H., Furuya,T., Takahashi,M., Kikkawa,E., Omura,Y., Abe,K.,
Kamihara,K., Katsuta,N., Sato,K., Tanikawa,M., Yamazaki,M.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Wakamatsu,A., Ishii,S.,
Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K., Nishikawa,T.,
Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y., Sekine,M.,
Kikuchi,H., Murakawa,K., Kanehori,K., Takahashi-Fujii,A.,
Oshima,A., Sugiyama,A., Kawakami,B., Suzuki,Y., Sugano,S.,
Nagahari,K., Masuho,Y., Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2343
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI2000685"
/clone_lib="NT2RI2"
/db_xref="H-InvDB:HIT000012711"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 550..2235
/codon_start=1
/protein_id="BAG51624.1"
/translation="MADHNPDSDSTPRTLLRRVLDTADPRTPRRPRSARAGARRALLE
TASPRKLSGQTRTIARGRSHGARSVGRSAHIQASGHLEEQTPRTLLKNILLTAPESSI
LMPESVVKPVPAPQAVQPSRQESSCGSLELQLPELEPPTTLAPGLLAPGRRKQRLRLS
VFQQGVDQGLSLSQEPQGNADASSLTRSLNLTFATPLQPQSVQRPGLARRPPARRAVD
VGAFLRDLRDTSLAPPNIVLEDTQPFSQPMVGSPNVYHSLPCTPHTGAEDAEQAAGRK
TQSSGPGLQKNSPGKPAQFLAGEAEEVNAFALGFLSTSSGVSGEDEVEPLHDGVEEAE
KKMEEEGVSVSEMEATGAQGPSRVEEAEGHTEVTEAEGSQGTAEADGPGASSGDEDAS
GRAASPESASSTPESLQARRHHQFLEPAPAPGAAVLSSEPAEPLLVRHPPRPRTTGPR
PRQDPHKAGLSHYVKLFSFYAKMPMERKALEMVEKCLDKYFQHLCDGLEVFAAHAGRK
TVKPEDLELLMRRQGLVTDQVSLHVLVERHLPLEYRQLLIPCAYSGNSVFPAQ"
BASE COUNT 525 a 687 c 686 g 445 t
ORIGIN
1 acccatgctg actctggagc gggccgggtg gcgctgccca tccccggatc cttcgtagat
61 ggagtcttgc ttagtcgccc aagctggagt agtgtggctc gatctcagct ctgcaacctc
121 tgcctcccag ggttcaagca attctcctgc ctcagtttcc agagtagctg gaattatagt
181 gcagattctg ggaaagtgaa ctccacggaa gactcagaaa ccaagttcta attcagttgc
241 catgaaaact taaggtgaga ggattgtcaa ccagtgaaaa atacggagga aaacactaac
301 agaatatgat gaagaagcca agacttgggc tgcttgcatt acattaaccg aggtttaccc
361 gcagcgaggc tgcctccaaa gcatccggag tttgcaacca tcactgtcca ccccgcgagc
421 tgcgatagtg cgagaactac aattcccgtg gggctgtgcg ggagcatcgc gagagttaca
481 ttaactgtgg cggcgggaag gcggagcctg cagctggctg ggcggttagg agggcccggg
541 gccgagacga tggctgacca caaccctgac agcgactcca cgccgcgcac gctgctgcga
601 cgcgtgctgg atacagcgga cccgcgcacc ccgcggcgac cccggagtgc tcgggctgga
661 gcccggagag ccctgcttga aacggcttcc cccaggaagt tgagtggcca aacaaggacg
721 atagccagag ggcgttccca tggagccagg tctgttggca gatcggccca tattcaggcc
781 agtgggcact tggaggaaca gacacctcgg acgctgctga agaacatcct actaactgcc
841 ccagaatctt ccatcctgat gcctgagtcg gtagtgaagc cagtgccagc accgcaggcg
901 gtccaaccct ccagacaaga gagcagttgc ggcagcctgg agctgcaact tcctgagctc
961 gagcccccca caaccctggc tccaggtctg ctggcccctg gcaggaggaa acagaggctg
1021 agactgtcag tgtttcagca gggagtggac caggggctgt ctctctccca agagcctcaa
1081 gggaatgctg atgcctcttc cctcaccaga tccctcaacc tgacctttgc cacacctctt
1141 cagccacagt cagtgcagag gcctggcttg gcccgcagac ctccagcccg ccgagctgta
1201 gacgtgggtg cctttttgcg ggatctgcga gatacttccc tggctcctcc aaacattgtg
1261 ttggaggaca cccagccgtt ctctcagccc atggttggct cccccaacgt gtatcactcc
1321 ctgccctgca cgcctcacac tggggctgaa gacgctgagc aggctgccgg tcgcaagaca
1381 cagagcagtg ggcctgggct gcagaagaat agccctggga aaccagccca gtttctggca
1441 ggagaggcag aggaggtcaa tgcctttgct ctgggcttcc tgagcaccag cagtggtgtc
1501 tctggagaag atgaagtaga gcccttacac gatggagttg aagaggcaga gaaaaagatg
1561 gaagaagaag gtgtgagtgt gagtgaaatg gaggcaacag gagcacaagg acccagcagg
1621 gtagaagagg ctgagggaca cacagaggtg acagaagcag agggatccca ggggactgct
1681 gaggctgacg ggccaggagc atcttcaggg gatgaggatg cctctggcag ggcagcaagt
1741 ccagagtcgg cctccagcac ccctgagtct ctccaggcca ggcgacatca tcagtttctt
1801 gagccagccc cagcgcctgg tgctgcagtc ttatcttcag agcctgcaga gcctctgttg
1861 gtcaggcatc cccctaggcc ccggaccacc ggccccaggc cccggcaaga tccccacaag
1921 gctggactga gccactatgt gaaactcttt agcttctatg ccaagatgcc catggagagg
1981 aaggctcttg agatggtgga gaagtgccta gataaatatt tccagcatct ttgtgatggt
2041 ctggaggtat ttgctgctca tgctggccgc aagactgtga agccagagga cctggagctg
2101 ctgatgcggc ggcagggcct ggtcactgac caagtctcac tgcacgtgct agtggagcgg
2161 cacctgcccc tggagtaccg gcagctgctc atcccctgtg catacagtgg caactctgtc
2221 ttccctgccc agtagtggcc aggcttcaac actttccctg tccccacctg gggactcttg
2281 cccccacata tttctccagg tctcctcccc acccccccag catcaataaa gtgtcataaa
2341 cag
//